Effects of aspirin and clopidogrel on neural stem cells

Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ischemic stroke. We investigated the effects of aspirin and clopidogrel on neural stem cells (NSCs). NSCs were dissociated from fetal rat cortex and cultured with basic fibroblast growth factor and N2 medium. To measure the effects of aspirin and clopidogrel on NSCs, NSCs were treated with several concentrations of aspirin, clopidogrel bisulfate, and clopidogrel resinate for 24 h. After the treatment, we measured cell viability by cell counting kit-8, MIT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, trypan blue staining, flow cytometry, and lactate dehydrogenase assay. To evaluate their effects on NSC proliferation, we performed BrdU cell proliferation assay and colonyforming unit assay. We compared the intracellular protein level in the NSCs treated with aspirin and two types of clopidogrel, by proteomics analysis. Various viability tests showed that clopidogrel resinate and clopidogrel bisulfate did not affect the viability and proliferation of NSCs whereas aspirin decreased them even at low concentrations which are clinically relevant. Moreover, through the proteomics, it was confirmed that the toxicity of aspirin to NSCs might be associated with the alteration of several intracellular proteins. Taken together, these results suggest that clopidogrel resinate and clopidogrel bisulfate are safe but aspirin could be toxic to NSCs. Therefore, when these antiplatelet agents are prescribed over the long-term, the finding that aspirin could be toxic to NSCs should be considered.This work was supported by the Basic Science Research Program of the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (2015R1A2A2A04004865), by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C2160), and by Korea Drug Development Fund (KDDF) funded by Ministry of Science and ICT, Ministry of Trade, Industry & Energy and Ministry of Health & Welfare (KDDF-201609-02, Republic of Korea)

Characteristics of the electron beam from SAFE microcolumn system

학위논문(석사)--서울대학교 대학원 :물리학과,1996.Maste

비예혼합 와환에서의 화염 전파에 관한 실험적 연구

학위논문(석사)--서울대학교 대학원 :기계공학과,1998.Maste

An Empirical Study on Training Transfer of the Civil Servants in Korea Local Government

본 연구에서는 교육훈련 전이에 영향올 미치는 제 요인들을 국내 · 외 선행연 구의 문헌검토를 통해 도출하여 지방자치단체의 공무원들을 대상으로 실증적으 로 규명하였다. 선행연구 검토 결과 본 연구에서는 교육훈련 전이에 영향을 미치 는 제 요인들로 크게 두 가지 요인，즉 교육훈련 프로그램의 설계요인으로서 강 사의 자질，교육내용，피훈련자의 개인적 특성(학습동기，성취욕구，자기유능감)과 조직적 환경요인으로 상사 및 동료의 지원，지속적 학습문화로 설정한 뒤，설문 조사를 실시하였고 조사결과를 요인분석과 회귀분석을 통해 분석하였다. 분석결과，교육훈련 프로그햄의 설계요인과 조직적 환경요인이 공무원 교육훈 련 전이에 대해 통계적으로 유의미한 긍정적인 영향을 미친다는 결론을 얻었다