加强新时代教育科学研究工作（笔谈）

2019年11月,教育部印发《教育部关于加强新时代教育科学研究工作的意见》(以下简称《意见》),这是新中国成立以来教育部印发的首个教育科研规范性文件,对做好新时代教育科研工作具有重大开创性意义。《意见》是指导新时代教育科学研究工作的纲领性文件,在进一步明确了新时代教育科研工作的指导思想、目标任务和思路举措的同时,还为做好新时代教育科研工作提供了强大的政策支持和制度保障。为进一步学习好《意见》精神,推动建设具有中国特色、世界水平的教育科学理论体系,不断提升教育科研质量和服务水平,《中国高教研究》编辑部与厦门大学高等教育发展研究中心邀请高等教育研究领域的六位专家学者,从不同角度为新时代加强高等教育科学研究工作贡献智慧

Construction of SMART cDNA library from kidney of large yellow croaker (Pseudosciaena crocea) stimulated with the inactivated trivalent bacteria vaccine

国家自然科学基金资助项目(40476052);; 国家海洋局海洋科学基金项

自然种群唐鱼的耳石、日龄与生长

为了解唐鱼自然种群的年龄结构和生长特征,根据耳石结构于2005年6月—2006年4月每2个月1次对栖息于从化市鹿子田附近自然水体唐鱼的日龄组成进行了调查。2005年6月采集到的唐鱼日龄集中在40～70d,8月和10月唐鱼种群既有较大个体,也有孵出不久的仔鱼,日龄分布在10～130d。2005年12月和2006年2月大部分唐鱼日龄在50d以上,2006年4月大部分唐鱼日龄则在50d以下。与人工养殖的唐鱼相比,自然种群唐鱼耳石生长轮的清晰度和对比度高,但生长轮宽度规则性较低。自然种群唐鱼的耳石长径与体长呈显

区域研究:全球变化研究的重要途径

区域研究是全球变化研究的重要途径。国际上南部非洲、南亚、东亚、东南亚4个地区及若干重大区域研究项目如LBA、AMMA、MAIRS及ProMed等典型案例研究表明:区域研究是全球变化研究的基础,区域的实验、模拟、分析是全球环境变化研究的有效方法。区域研究应关注:陆地表面过程、海岸带的陆—海相互作用过程、区域气候变化过程等关键过程对全球变化的响应及作用;区域边缘现象、阈值与突变问题;区域人类活动对全球变化的有序适应等。有必要制订国际区域研究计划,实施地球系统的区域观测,建立区域研究的集成新方法、区域实验与尺度转换方法,建立基于数理基础的区域地球系统模型和数值模拟等新方法

Method for liver tissue metabolic profiling based on liquid chromatography

Hepatocellular carcinoma (HCC) is one of the most frequent malignancy worldwide. Evaluation of tissue metabolites is with significant value in HCC study which can provide more direct information of metabolic disorder compared with biofluids. A protocol for the metabolic profiling of liver tissue was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS). According to the design of experiment (DOE), methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10–5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15% and the recovery of IS was from 84.8% to 109.1%. After method validation, this method was applied to HCC study. Liver tissue samples were collected from HCC patients and each sample group involved carcinoma tissue, adjacent noncancerous tissue and distal noncancerous tissue, respectively. The data demonstrated that noncancerous tissues from the adjacent and distal were nearly identical, but were greatly different from the carcinoma tissue. After the removal of missing values, totally 880 significantly changed ions between the carcinoma tissue and distal noncancerous tissue group were filtered out. 44 metabolites in the ESI positive mode and 65 in the negative mode were identified by databases and some of them were further confirmed by authentic standard samples. Several important metabolic pathways were clarified. The result proved that the established method was adequate for liver and other tissues metabolic profiling analysis.Hepatocellular carcinoma (HCC) is one of the most frequent malignancy worldwide. Evaluation of tissue metabolites is with significant value in HCC study which can provide more direct information of metabolic disorder compared with biofluids. A protocol for the metabolic profiling of liver tissue was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS). According to the design of experiment (DOE), methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10–5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15% and the recovery of IS was from 84.8% to 109.1%. After method validation, this method was applied to HCC study. Liver tissue samples were collected from HCC patients and each sample group involved carcinoma tissue, adjacent noncancerous tissue and distal noncancerous tissue, respectively. The data demonstrated that noncancerous tissues from the adjacent and distal were nearly identical, but were greatly different from the carcinoma tissue. After the removal of missing values, totally 880 significantly changed ions between the carcinoma tissue and distal noncancerous tissue group were filtered out. 44 metabolites in the ESI positive mode and 65 in the negative mode were identified by databases and some of them were further confirmed by authentic standard samples. Several important metabolic pathways were clarified. The result proved that the established method was adequate for liver and other tissues metabolic profiling analysis

黑龙江省中俄界江旅游发展策略研究

在东北亚区域旅游合作日益密切的背景下,中俄两国边境旅游业得到了持续迅速的发展。依托资源、地缘和区位优势,黑龙江省中俄界江旅游已成为中俄边境旅游合作的重要组成部分。随着东北亚地区经济一体化的不断推进,中俄界江旅游发展水平得到了巨大推进。在分析旅游资源概况的基础上,探讨了中俄界江旅游业发展现状和存在问题,并提出了其旅游业发展的主要策略:打造边境旅游、邮轮旅游、冰雪旅游、文化旅游和生态观光旅游等特色旅游产品;构建一带两级三区的旅游空间结构布局,即一条界江旅游带,黑河和抚远两个旅游增长极,漠河、嘉荫—萝北和兴凯湖三个旅游集合区;完善旅游市场营销策略;健全旅游交通和公共服务体系

一种基于HPLC-MS的代谢组学新分析方法

Novel Analytical Methods of HPLC-MS Based Metabolomic