Serrs and Electrochemical Study of Yeast Iso-1-Cytochrome c Mutant F82H

应用表面增强拉曼光谱和循环伏安法研究了细胞色素c及其突变体F82H的氧化还原性质 .结果表明苯丙氨酸对组氨酸的取代使得蛋白质结构更为稳定 .相对于原体蛋白质 ,突变体的氧化还原电位向负电位方向移动 ,这被归因于由氧化还原过程中伴随有配体转换反应影响所致 .The redox properties of iso?1?yeast cytochrome c and its mutant F82H were studied by surface?enhanced Raman spectroscopy and cyclic voltammetry. The results showed that the replacement of phenylalanine?82 with histidine led to a more stable global structure of the protein. A negative shift in the redox potential of the mutant relative to that of wild type protein is ascribed to a ligand switching reaction during the redox processes.作者联系地址：苏州大学化学系!江苏苏州215006,苏州大学化学系!江苏苏州215006,中国科学院长春应用化学研究所!吉林长春130022,Dept.of Chem.!Iowa State Univ.,Ames,IA50011,USA,Dept.of Chem.!Iowa State Univ.,Ames,IA50011,USAAuthor's Address: 1. Dept. of Chem., Suzhou Univ., Suzhou 215006, China; 2. Inst. of Changchun Applied Chem.,Chinese Academy of Sci.,Changchun 130022, China; 3. Dept.

磷钼酸pma修饰炭载pd催化剂对甲酸氧化的电催化性能

为了提高直接甲酸燃料电池（DFAFC）中炭载Pd（Pd/C）催化剂对甲酸氧化的电催化性能,用回流法制备了磷钼酸（PMA）修饰的炭载Pd（PMA-Pd/C）催化剂.并用谱学技术和电化学技术表征了催化剂的组分和结构,发现PMA通过化学作用而牢固地固定在Pd表面.由于PMA-Pd/C催化剂能抑制甲酸的自分解,因此,PMA-Pd/C催化剂对甲酸氧化的电催化性能优于Pd/C催化剂

Study of Electrochemical Behaviour of Myoglobin Using Cyclic Voltammetry and Synchronous Fluorescence Spectroscopy

用循环伏安法和同步荧光光谱技术研究了肌红蛋白的电化学行为，实验结果表明，高铁肌红蛋白分子中至少存在一个可调节分子构象变化的氧分子，而且长时间通入高纯氮气可以除掉高铁肌红蛋白分子内的这个氧，当高铁肌红蛋白分子内的氧被彻底除去后，用循环伏安法可以观察到肌红蛋白在三氧化二铟电极上的准可逆的电学反应。同步荧光光谱实验表明，高铁肌红蛋白在彻底除氧后，分子构象发生了变化，而且这种构象变化是可逆的。Myoglobin undergoes irreversible heterogeneous electron transfer reactions at bare metal electroeds. Thus, much effort has been devoted to obtain quasi-reversible electrochemical reactions of myoglobin at metal electrodes modified with mediators￣(1～3). However, the electron transfer rates at the above modified electrodes is very small so that the spectroelectrochemical techniques should be used in the most of studies. Recently, Taniguchi et al￣[4]. reported that a quasi-reversible electrochemical reaction of the purified horse heart myoglobin was observed at an indium oxide electrode with cyclic voltammetry technique. It was suggested that high purification of myoglobin may be the main reason for obtaining a quasi-reversible electroChemical reaction of myoglobin in the cyclic voltammetry experiments. In this papor, it was reported for the first time that a pair of well-defined redox peaks were oberved in the CV of the commercially available horse heart myoglobin without further purification at an indium oxide electrode after oxygen was eliminated from the solution and the molecules of the oxldized form of myoglobin, i. e.metmyoglobin.作者联系地址：中国科学院长春应用化学研究所电分析开放实验室Author's Address: Laboratory of Electroanalytical Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, 130022, P. R. Chin

直接甲酸燃料电池炭载Pd阳极催化剂的稳定性

用x射线衍射和电化学方法研究了在甲酸溶液中浸泡一段时间后的Pd／C催化剂的结构和电催化性能，发现在甲酸溶液中浸泡15d后，Pd／C催化剂中Pd粒子的相对结晶度由1．73增加到3．34，平均粒径由4．4nm降低到1．8nm，对甲酸氧化的电催化活性和稳定性降低，甲酸氧化的峰电流密度由9．3mA／cm2降低到6．7mA／cm2。这可能是由Pd／C催化剂中的Pd在甲酸中会有一定的溶解和Pd／C催化剂能催化分解甲酸引起的

直接甲酸燃料电池Pd阳极催化剂及其电催化稳定性

直接甲酸燃料电池（DFAFC）中Pd阳极催化剂对甲酸氧化具有很好的电催化活性,但电催化稳定性较差,因此,对Pd催化剂电催化活性和稳定性的影响原因和机理的研究已经成为DFAFC阳极催化剂的研究重点,本文综述了DFAFC中Pd催化剂和Pd基复合催化剂的研究和发展概况.主要介绍了Pd催化剂的优缺点、稳定性及提高稳定性的方法和机理等,为Pd催化剂和Pd基复合催化剂的实际应用奠定基础

The Influnce of Conjugated π Bond in Promoter Molecule on the Direct Electrochemical Reaction of Cytochrome C

比较了具有单功能基团的共轭有机小分子噻吩和四氢噻吩对细胞色素ｃ直接电化学反应的促进作用。发现四氢噻吩不能加速细胞色素ｃ的直接电化学反应。而噻吩是一种很好的促进剂。表明噻吩分子中的共轭π键在加速细胞色素ｃ的电子传递过程中起着重要作用。The promoter effect of thiophene with only one-functional group on the directelectrochemical reaction of cytochrome c was compared with that of tetrahydrothiophene in this report.It was found that thiophene is a good promoter.But tetrahydrothiophene could not facilitate the directelectrochemistry of cytochrome c although this compound is very similar to thiophene in str uctureexcept for conjugated π bond.This demonstrated that the conjugated π bond in thiophene plays animportant role for the acceleration of the direct electrochemistry of cytochrome c.作者联系地址：中国科学院长春应用化学研究所电分析化学开放实验室Author's Address: Laboratory of Electroanalyticat Chemistry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 13002

Direct Electrochemical Reaction of Cytochrome c at the Poly (Ethylene Oxide) Film modified Gold Electrodes

本文报道了细胞色素Ｃ在聚乙烯氧化物（ＰＥＯ）修饰的金电极上的直接电化学行为，发现ＰＥＯ是细胞色素Ｃ电化学反应的促进剂，ＰＥＯ修饰膜的形态对细胞色素Ｃ电化学反应的可逆性有较大的影响．In this paper, the direct electrochemical reaction of cytochrome c was sudied at the poly (ethylene oxide) film modified gold electrodes. It was found the PEO is a good promoter for the electrochemical reaction of cytochrome c, and the reversibility of reaction of cytochrome c was found to depend on the molecular weight of PEO and the morphology of the PEO films.作者联系地址：中国科学院长春应用化学研究所,南京师范大学化学系Author's Address: Changchun Inst.of Applies Chem.,Chinese Academy of Sci.,Changchun 130022 Yang Hui, Lu Tianhong * Dept. of Chem., Nanjing Normal Univ.,Nanjing 21009

Electrocatalytic Performance of Carbon Supported Au-Ir Catalyst as Cathodic Catalyst in Direct Formic Acid Fuel Cell

用四氢呋喃(THF)络合还原法分别合成并比较了碳载金(Au/C)、碳载铱(Ir/C)、碳载金-铱(Au-Ir/C)催化剂对氧气还原和甲酸氧化的电催化活性.发现3种催化剂对甲酸氧化都没有电催化活性;Au-Ir/C催化剂对氧还原的电催化活性要远好于Au/C和Ir/C催化剂.表明Au-Ir/C催化剂适合作为直接甲酸燃料电池(DFAFC)的阴极催化剂.In this paper,it is reported for the first time that the electrocatalytic activities of the carbon supported Au(Au/C),carbon supported Ir(Ir/C) and the carbon supported Au-Ir(Au-Ir/C) catalysts prepared in the aqueous solution with tetrahydrofuran(THF) for the oxidation of formic acid and for the oxygen reduction were compared.It was found that all three catalysts showed no electrocatalytic activity for the oxidation of formic acid,but the electrocatalytic activity of the Au-Ir/C catalyst for oxygen reduction was much better than those of Au/C and Ir/C.Thus,the Au-Ir/C catalyst is suitable to be used as the cathodic catalyst in Direct formic acid fuel cell(DFAFC).作者联系地址：南京师范大学化学与环境科学学院;Author's Address: 1.College of Chemistry and Environmental Science,Nanjing Normal University,Nanjing 210097,China;2.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,Chin

Effect of Au at Different States on Electrocatalytic Performance of Pd Catalyst for Oxidation of Formic Acid

合成并比较了碳载Au(Au/C)、碳载Pd(Pd/C)、碳载高合金化Pd-Au(Pd-Au/C-T)和碳载非合金化Pd-Au(Pd-Au/C-H)催化剂对甲酸氧化的电催化活性和稳定性.结果表明,Au/C对甲酸氧化基本没有电催化活性,而Pd/C对甲酸氧化有较好的电催化性能,Au的加入能进一步提高Pd催化剂对甲酸氧化的电催化活性和稳定性,特别是Pd-Au/C-T对甲酸氧化的电催化活性和稳定性要好于Pd-Au/C-H,更远好于Pd/C催化剂.相关反应机理有待进一步揭示.Using liquid-reduction or complex-redution method,four catalysts,i.e,the Au/C,Pd/C,Pd-Au/C-T and Pd-Au/C-H were preparated and which electroanalytic activities and stabilities for the oxidation of formic acid were compares.It was found that the Au/C catalyst has no electrocatalytic activity,and the Pd/C catalyst possesses the good electrocatalytic performance for the oxidation of formic acid.The addition of Au into the Pd/C catalyst can increase the electrocatalytic activity and stability.Especially,the electrocatalytic activity and stability of the Pd-Au/C-T catalyst with the alloy of Pd and Au are better than that of the of the Pd-Au/C-H catalyst,in which,Pd and Au do not form the alloy.The investigation of its reason is underway.作者联系地址：南京师范大学化学与环境科学学院,南京师范大学化学与环境科学学院,南京师范大学化学与环境科学学院 江苏南京210097,江苏南京210097,江苏南京210097Author's Address: College of Chemistry and Environmental Science,Nanjing Normal University,Nanjing 210097,Chin