Effects of Indoor and Outdoor Cultivation Conditions on the Cell Growth and Lutein Accumulation of Thermo-tolerant Desmodesmus sp.F51

在室内培养耐温微藻F51,考察了培养温度、自来水及非灭菌培养基对细胞生长及叶黄素积累的影响,并与50 L柱状光生物反应器户外分批培养情况进行对比.结果表明,藻株在35℃下的生物质产率和叶黄素产率最大,分别为767.5和3.05mg/(L·d).低温培养有利于叶黄素积累,但生长速率减缓.用自来水及非灭菌培养基培养时,藻株无明显的生长抑制及染菌现象,在13.0~27.6℃户外培养温度下,藻株最高生物质产率、二氧化碳固定率和叶黄素产率分别为236.3,444.2和1.17 mg/(L·d).The effects of temperature, local tap-water and culture medium without sterilization on cell growth and lutein production of Desmodesmus sp. F51 were investigated under indoor conditions. Outdoor batch cultivation of Desmodesmus sp. F51 was also investigated in 50 L cylindrical photobioreactor. The results showed that the best biomass productivity [767.5 mg/(L·d)] and lutein productivity [3.05 mg/(L·d)] occurred at a temperature of 35 ℃. A low temperature enhanced the lutein content but reduced cell growth rate. There is no significant growth inhibition and microbiological contamination were observed for Desmodesmus sp. F51 using local tap-water and culture medium without sterilization. Under the outdoor temperature of 13.0~27.6 ℃, the highest biomass productivity, CO2 fixation rate and lutein productivity of 236.3, 444.2 and 1.17 mg/(L·d) were obtained, respectively,.福建省青年科技人才创新基金资助项目(编号:2016J05077);; 福建省教育厅资助项目(编号:JA15077

Effect of Nitrate Pulse-Feeding Cultivation on Cell Growth and Cell Composition of Thermo-Tolerant Desmodesmus sp. F51

考察脉冲式添加氮源培养对耐温微藻Desmodesmus sp.; F51细胞生长和细胞组成的影响。结果表明,在脉冲式添加氮源培养过程中,藻株F51的蛋白质含量由(56016)mg/g降低至(45617)mg/g; ,而碳水化合物和油脂含量则分别由(20913)mg/g和(983); mg/g提高至(31012)mg/g和(1204)mg/g。另外,对色素和油脂的具体组成变化进行分析,发现脉冲式添加氮源培养可增强beta-胡萝; 卜素的生物合成,以及促进alpha-胡萝卜素生物转化为叶黄素,同时还可发现叶黄素积累与多不饱和脂肪酸含量变化呈正相关性。而在碳水化合物的具体组成; 分析中,发现碳水化合物为藻株F51主要的储能物质,当脉冲式添加氮源培养时间为3; d时,其葡萄糖占总碳水化合物的质量分数可明显提高至(75.71.4)%。采用脉冲式添加氮源培养策略可明显同时促进藻株F51叶黄素和碳水化合物的生; 产。The aim of the present study was to investigate the effect of nitrate; pulse feeding on the cell growth and cell composition of Desmodesmus sp.; F51. Under nitrate pulse-feeding cultivation, the protein content of; cells of Desmodesmus sp. F51 decreased from(560 16) mg/g to(456 17); mg/g, while the contents of carbohydrate and lipid increased from(209 ; 13) mg/g to(310 12) mg/g and(98 3) mg/g to(120 4) mg/g, respectively.; By investigating the changes in carotenoid, lipid and carbohydrate; compositions, it appeared that nitrate pulse feeding could promote; beta-carotene biosynthesis and enhance the bioconversion of; alpha-carotene to lutein. Lutein accumulation was positively associated; with polyunsaturated fatty acid formation. Desmodesmus sp. F51 tended to; accumulate carbohydrate as an energy storage product after 3 days of; cultivation under nitrate pulse-feeding conditions, and the major; accumulated monosaccharide was glucose(75.7 1.4)%. Therefore, the; nitrate-pulse feeding strategy is a highly promising approach to; simultaneously produce lutein and carbohydrate in Desmodesmus sp. F51.国家自然科学基金应急管理项目; 福建省青年科技人才创新基金资助项目; 福建省海洋高新产业发展专项; 福州市市校(院所)科技合作项