Curcumin-induced Apoptosis Affects the Expression and Localization of hnRNP A2/B1 in Human Osteosarcoma MG-63 Cells

姜黄素(CurCuMIn)诱导处理的人成骨肉瘤Mg-63细胞,在光镜和电镜观察细胞凋亡的基础上,对HnrnP A2/b1在核基质中存在、分布及其与凋亡相关基因产物在Mg-63细胞中的共定位关系进行了研究.经姜黄素处理后,细胞出现染色质凝聚、细胞核固缩、凋亡小体等典型的细胞凋亡形态特征;双向凝胶电泳和质谱鉴定结果显示,HnrnP A2/b1存在于Mg-63细胞核基质蛋白组分中,在姜黄素处理后细胞核基质蛋白中表达下调.蛋白质印迹杂交结果,证实HnrnP A2/b1在姜黄素处理前后的Mg-63细胞核基质蛋白中的存在及其表达下调变化.免疫荧光显微镜观察显示,HnrnP A2/b1定位于Mg-63细胞核基质纤维上,经姜黄素处理后出现分布位置与表达水平变化.激光扫描共聚焦显微镜的观察结果显示,HnrnP A2/b1在Mg-63细胞凋亡过程中与bAX、bCl-2、fAS和P53等基因产物具有共定位关系,且其共定位区域发生了变化.研究结果证实了HnrnP A2/b1定位于核基质纤维上,是一种核基质蛋白,在姜黄素诱导人成骨肉瘤Mg-63凋亡过程中的表达与分布变化及其与凋亡相关基因的关系显然对Mg-63细胞凋亡具有重要影响,这为深入揭示肿瘤细胞凋亡的机制提供了重要科学依据和深入探索的新方向.The morphology of human osteosarcoma MG-63 cells under light microscopy and transmission election microscopy was altered when treated with curcumin.The distribution of heterogeneous nuclear ribonucleoprotein (hnRNP)A2 /B1 in the nuclear matrix and its colocalization with the apoptosis-related gene products were examined.The results demonstrated that curcumin induced typical apoptotic responses,such as chromatin agglutination,nuclei condensation,and apoptotic body formation.Twodimentional PAGE and mass spectrum analyses showed that hnRNP A2 /B1 was complexed with the nuclear matrix.Confirmed by Western blotting,curcumin treatment resulted in a down-regulation of hnRNP A2 /B1 expression.An altered distribution of hnRNP A2 /B1 with the nuclear matrix was observed in immunofluorescence assays.Laser confocal microscopy showed that hnRNP A2 /B1 colocalized with apoptosis-related molecules,including Bax,Bcl-2,Fas and p53,with different patterns before and after curcumin treatments.Our study showed that expression level and the distribution of hnRNP A2 /B1,a nuclear matrix protein,were altered by curcumin treatments and mought be involved in the process of apoptosis in MG-63 cells.国家自然科学基金项目(No.30871241)---

A unique NH-spacer for N-benzamidothiourea based anion sensors. Substituent effect on anion sensing of the ICT dual fluorescent N-(p-dimethylaminobenzamido)-N '-arylthioureas

A series of N-(p-dimethylaminobenzamido)-N'-(substituted-phenyl)thioureas (substituent = p-CH3, H, p-Cl, p-Br, m-Br, m-NO2, and p-NO2) were designed as anion sensors in order to better understand the -NH-spacer via a substituent effect investigation. In these molecules the dual fluorescent intramolecular charge transfer (ICT) fluorophore p-dimethylaminobenzamide as the signal reporter was linked to the anion-binding site, the thiourea moiety, via an N-N single bond. Correlation of the NMR signals of the aromatic and -NH protons with substituents in these molecules indicated that the N-N single bond stopped the ground-state electronic communication between the signal reporter and the anion-binding site. Dual fluorescence was observed in highly polar solvents such as acetonitrile with the former five derivatives. The fact that the CT emission wavelength and the CT to LE emission intensity ratio of the sensors were independent of the substituent existing in the anion-binding moiety suggested that the substituent electronic effect could not be communicated to the CT fluorophore in the excited-state either. Yet in acetonitrile both the CT dual fluorescence and the absorption of the sensors were found to be highly sensitive toward anions. A conformation change around the N-N bond in the sensor molecules was suggested to occur upon anion binding that established the electronic communication between the signal reporter and the anion-binding site. The anion binding constants of the N-(p-dimethylaminobenzamido)thiourea sensors were found higher than those of the corresponding traditional N-phenylthiourea counterparts and the substituent effect on the anion binding constant was much higher than that in the latter. "-NH-" was shown to be a unique spacer that affords N-benzamidothiourea allosteric anion sensors

有穷状态XYZ/E程序的模型检查研究

XYZ/E语言是一个可执行的时序逻辑语言,可以用来描述有穷状态反应系统.为了构造一个以XYZ/E语言作为系统描述语言的模型检查工具,可以在已有的成熟模型检查工具的基础上,实现一个语言转换工具,把对反应系统的XYZ/E语言描述转换成该模型检查工具可接受的描述,然后利用该工具进行模型检查.SPIN是一个得到广泛应用的线性时序逻辑模型检查工具,能够验证由若干个进程异步组合而成的有穷状态系统,它使用进程元语言Promela来描述反应系统.我们实现了一个语言转换工具,可以把满足一定限制的XYZ/E程序转换成Promela程序,从而可以利用SPIN来对用XYZ/E语言描述的有究状态反应系统进行模型检查,达到了能够对用XYZ/E语言描述的有穷状态系统进行模型检查的目的.Finite-state reactive systems arise naturally in several areas of computer science, particularly in the design of digital circuits and communication protocols. These systems are characterized by ongoing, typically nonterminating and highly nondeterministic behavior. Ofen such systems amount to parallel or distributed programs. During the past twenty years, temporal logic model checking has became the most thoroughly researched technology for automatic verification of finite-state reactive systems. In this approach specifications are expressed in a propositional temporal logic, and systems are modeled as a state-transition systems. An efficient search procedure is used to determine automatically if the specifications are satisfied by the transtion systems. Typically, the user provides a high level representation of the model and the specification to be checked. The model checking algorithm will either terminate with the answer true, indicating that the model satisfies the specification, or give a counterexample execution that shows why the formula is not satisfied. The counterexamples are particularly important in finding subtle errors in complex transition systems. Being an executable temporal logic language, XYZ/E can be used to describe finite-state reactive systems. To model-checking a finite-state system described by XYZ/E program, we can reimplement a model checking tool, which using XYZ/E as its system description language, or implement a language-translation tool, which can translation a system described by a XYZ/E program into the same system but described in another language, to utilizing an existing model checker, we choose the latter. Our goal is to model checking systems described by XYZ/E programs. For this end, we have implemented a language translation tool, which can translate a given system described by a XYZ/E program into the same system described by a Promela program, which can be model checked by the famous LTL model checker SPIN

5CrMnMo激光熔凝热疲劳性能实验研究

研究了激光熔凝强化对5CrMnMo基材热疲劳性能的影响。实验结果表明激光熔凝强化显著提高了材料抵抗热疲劳裂纹萌生和扩展的能力

Fe-Cr-C-Wi-Ni激光熔覆涂层力学行为与微观组织关系的研究

利用SEM,TEM研究了Fe-Cr-C-W-Ni激光熔覆涂层的微观组织。利用显微硬度仪及冲击动载磨损实验机研究了涂层的显微硬度、高温回火稳定性及抗冲击磨损性能等。实验结果表明,激光熔覆涂层具有高的显微硬度、良好的高温回火稳定性及优良的耐磨损性能,这些力学行为与涂层的微观组织密切相关

铁基抗高温磨损激光熔覆涂层强韧设计和研究Ⅰ:激光熔覆合金成分、微观结构强韧化设计及涂层制备

本文在对热工模具进行失效分析的基础上，利用激光熔覆技术，在５ＣｒＭｎＭｏ基础上设计并制备强韧兼备的抗高温磨损涂层

铁基抗高温磨损激光熔覆涂层强韧设计和研究 Ⅱ:Fe-Cr-C-W-Ni激光熔覆层的微观组织及性能

利用ＯＭ、ＳＥＭ、ＴＥＭ研究了Ｆｅ－Ｃｒ－Ｃ－Ｗ－Ｎｉ激光熔覆涂层熔覆态及其高温时效态的微观组织结构。结果表明激光熔覆层组织细小，具有强韧两相组成（奥氏体和Ｍ_７Ｃ_３碳化物）的微观结构特征，高温时效处理组织中有Ｍ_（２３）Ｃ_６、Ｍ_６Ｃ、Ｍ_２Ｃ等新碳化物形成。显微硬度和冲击磨损实验证实了激光熔覆态和峰值时效态熔覆层均具有良好的力学性能

铁基抗高温磨损激光熔覆涂层强韧设计和研究II:Fe-Cr-C-W-Ni激光熔覆层的微观组织及性能

本文以对热工模具进行失效分析的基础上，利用激光熔覆技术，在５ＣｒＭｎＭｏ基础上设计并制备强韧兼备的抗高温磨损涂层