Application of a novel domestic hypersensitive HBsAg reagent in blood screening

目的对1种新型国产超敏HbSAg试剂在血液筛查中的应用进行评估。方法用考核试剂WT ClEIA分别检测WHO标准品、HbV阴性的血清、各种HbV亚型及HbSAg突变株标本以及无偿献血者标本,并与参比试剂做比较。结果 WT ClEIA对HbSAg阴性标本的检测特异性为99.81%(95%CI:99.57%~99.93%);对WHO标准品检测的分析灵敏度可达0.012 Iu/Ml,高于参比试剂HEPAnOSTIkA HbSAg ulTrA的0.05 Iu/Ml和AbbOTT MurEX V3的0.1 Iu/Ml;对HbSAg突变株及不同亚型的检出率也高于2种参比试剂;对近5 000份标本的检测结果与参比试剂AbbOTT MurEX V3有高度的符合率(99.60%)。结论该试剂检测性能良好,在血液筛查中有较好的应用前景。Objective To study the application of a novel domestic hypersensitive HBsAg reagent in blood screening.Methods The reagent for evaluation named WT CLEIA together with reference reagents were used to test WHO standard,HBV negative samples and specimens with various HBV subtypes and HBsAg mutants.Results The specificity of WT CLEIA was 99.81%(95% CI: 99.57% ～ 99.93%).The analytical sensitivity of WT CLEIA was 0.012 IU / ml,which was higher than Hepanostika HBsAg Ultra(0.05 IU / mL) and Abbott Murex V3(0.1 IU / mL);WT CLEIA also had a higher detection rate of HBsAg mutants and subtypes than these 2 reference reagents.In addition,WT CLEIA had a high accordant rate with Murex V3 in blood donors HBsAg screening.Conclusion WT CLEIA has a good performance in HBsAg screening and would have a good prospect of application in blood screening.福建省自然科学基金面上项目(2011D007); 厦门市输血医学重点专科建设项目(2012-2014

Carbon cycling of forest ecosystems in response to global nitrogen deposition: a review

森林土壤和植被储存着全球陆地生态系统大约46%的碳,在全球碳平衡中起着非常重要的作用。过去几十年来,森林生态系统的碳循环和碳吸存受到了全球氮沉降的深刻影响,因为氮沉降改变了陆地生态系统的生产力和生物量积累。以欧洲和北美温带森林区域开展的研究为基础,综述了氮沉降对植物光合作用、土壤呼吸、土壤DOM及林木生长的影响特征和机理,探讨了森林生态系统碳动态对氮沉降响应的不确定性因素。热带森林C、N循环与大部分温带森林不同,人为输入的氮对热带生态系统过程的影响也可能不同,因此指出了在热带地区开展碳氮循环耦合研究的必要性和紧迫性。Forest soils and vegetation store about 46 percent of all carbon in the terrestrial biosphere, which play a critical role in global carbon balance. Increases in atmospheric nitrogen deposition over the last several decades have altered carbon cycling and sequestration of forest ecosystems by affecting productivity and biomass accumulation in terrestrial ecosystems. Based on the studies conducted in temperate forest regions in Europe and North America, the paper reviewed mechanism and characteristics underlying the impacts of nitrogen deposition on plant photosynthesis, soil respiration, soil dissolved organic matter (DOM), and tree growth, and the uncertainties as to the interactions between forest ecosystem carbon dynamics and nitrogen deposition were also analyzed and discussed. Most tropical forests function quite differently from temperate forests with regard to N and C cycling, and that the effects of anthropogenic N inputs on tropical ecosystem processes may also differ, so it is urgent and necessary to initiate studies related to carbon-nitrogen cycle coupling in the tropical regions.国家自然科学基金资助项目(30370259);; 福建省自然科学基金重点资助项目(B0320001)~

无偿献血者中隐匿性乙型肝炎病毒感染及表面抗原突变分析

采用多种免疫学检测和核酸检测相结合的方法调查了我国南方某城市无偿献血者中隐匿性乙型肝炎病毒(HBV)感染的存在情况。结果在9023例乙肝表面抗原(HBsAg)阴性的无偿献血者中,共发现17例HBV DNA阳性,隐匿性HBV感染者的发生率为0.19%(95%CI:0.11～0.30%)。序列分析显示其中6例在HBsAg"a"表位(aa124～aa147)存在不同程度氨基酸突变,突变发生率为42.9%(6/14,有3例未扩增出"a"表位片段序列),G145R突变是该地区隐匿性HBV携带者中发生频率最高的突变(4/6,66.7%)。隐匿性HBV感染者中基因型C的比例(10/17)显著高于HBsAg阳性的HBV感染者(0/15,P<0.01)

ST段抬高型急性心肌梗死院前溶栓治疗中国专家共识

急性心肌梗死仍然严重威胁我国人民健康,在我国广大城乡地区,形势更为严峻[1,2]。及时救治急性心肌梗死患者,降低死亡率和保护心脏功能刻不容缓。鉴于我国的实际情况,院前溶栓治疗在大城市以外的城乡地区具有重要意义。为此,中国医师协会胸痛专业委员会及中国医学救援协会心血管急救分会专门组织有关专家制订了本共识,旨在帮助院前医疗急救人员对急性心肌梗死患者选择最佳

Research Status and Development Trend of Mobile Manipulators

阐述了移动操作臂的研究现状和关键技术。移动操作臂在传统轻量高自由度机械臂的基础上结合自主移动平台,极大地扩展了灵活工作空间,提高了工作柔性,更适应复杂的工作环境和工作要求。通过分析几种典型移动操作臂的结构设计和功能指标,探讨了移动操作臂的本体结构设计、运动过程控制、路径规划、移动定位导航以及多移动操作臂协作等关键技术,展望了移动操作臂的发展趋势和应用前景