短程硝化/厌氧氨氧化/全程硝化工艺处理焦化废水

通过对短程硝化和厌氧氨氧化工艺的研究,开发了短程硝化/厌氧氨氧化/全程硝化(O1/A/O2)生物脱氮新工艺并用于焦化废水的处理。控制温度为(35±1)℃、DO为2.0～3.0mg/L,第一级好氧连续流生物膜反应器在去除大部分有机污染物的同时还实现了短程硝化。考察了HRT、DO和容积负荷对反应器运行效果的影响。结果表明,当氨氮容积负荷为0.13～0.22gNH 4+-N/(L.d)时,连续流反应器能实现短程硝化并有效去除氨氮。通过控制一级好氧反应器的工艺参数,为厌氧反应器实现厌氧氨氧化(ANAMMOX)创造条件。结果表明,在温度为34℃、pH值为7.5～8.5、HRT为33 h的条件下,经过115 d成功启动了厌氧氨氧化反应器。在进水氨氮、亚硝态氮浓度分别为80和90 mg/L左右、总氮负荷为160 mg/(L.d)时,对氨氮和亚硝态氮的去除率最高分别达86%和98%,对总氮的去除率为75%。最后在二级好氧反应器实现氨氮的全程硝化,进一步去除焦化废水中残留的氨氮、亚硝态氮和有机物。O1/A/O2工艺能有效去除焦化废水中的氨氮和有机物等污染物,正常运行条件下的出水氨氮<15 mg/L、亚硝态氮<1.0 mg/L,COD降至124～186 mg/L,出水水质优于A/O生物脱氮工艺的出水水质

过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探

The mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewate

The Production of Hydroxyl Radical in HRP-NADH-H2O2/O-2 Systems and Its Application in Chlorobenzene Removal

The mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewate