福建城市化发展战略博士笔谈

2003年5月,“福建省博士创业促进会”在福州举办了福建省城市发展战略“博士月谈”,与会者主要是省内高校和科研单位的专家、博士。会上,大家用书面发表意见,畅所欲言,崇论宏议。本刊精选部分意见,以飨读者。相信这些意见对福建城市化问题研究的深化将起到积极的推动作用

改良分子信标-双重实时荧光PCR快速检测SARS病毒

目的建立改良分子信标-双重实时荧光PCR检测SARS病毒的方法,用于SARS的早期诊断和动物溯源。方法利用改良分子信标技术、装甲RNA和双片段双色荧光技术,根据GenBank公布的SARS病毒聚合酶基因1b的阅读开放框架结构的保守序列,自行设计一对引物和探针,以部分临床标本的酶联吸附实验结果和传统细胞培养方法作为对照,建立分子信标检测SARS病毒的方法。对368份临床标本(咽漱液、血液、粪便、尿液)、52份细胞培养液和50份动物标本进行荧光PCR扩增。结果分子信标检测SARS病毒的方法灵敏度为10～100个拷贝ml,与流感病毒等呼吸道病毒无交叉反应。分子信标检测368份临床标本,20份阳性。其中确诊病例阳性率为21.27%(1047),确诊病例的咽漱液阳性率为43.48%,还分别从粪便和血清中检测到SARS病毒。52份细胞培养液,29份阳性,阳性率为55.77%。50份动物标本,23份阳性,阳性率为46%。结论改良分子信标-双重实时荧光PCR检测SARS病毒方法灵敏度高、特异性强,可用于SARS的临床早期诊断和动物溯源

Description on two species of Benedenian from Epinephelus awoara in Xiamen, Fujian, China(Monogenea: Capsalidae)

报道厦门海域网箱养殖的石斑鱼广泛流行感染的 2种贝尼登单殖吸虫病原 ,它们是 :康吉新贝尼登虫 N eobenedeniella congeri(Yamaguti,195 8) Yamaguti,1963和片卷涡异贝尼登虫Allobenedenia convoluta(Yamaguti,1937) Yamaguti,1963,均寄生于青石斑鱼 (Ep inephelusawoara) .这两种贝尼登虫均隶属于单殖吸虫目分室科 (Capsalidae)的贝尼登亚科 (Benedeniinae) .对这 2种贝尼登虫病原进行了形态记述和感染报道 ,并对这两种贝尼登虫病原在厦门海水养殖石斑鱼中的流行感染情况进行了简单介绍Two species of benedenian are reported to infect on the body surface of maricult ured fish in Xiamen (24°29′N, 118°03′E.), Fujian, China. Both of them belong to 2 different genus of Benedeniinae, Capsalidae respectively. Their host is Epinephelus awoara (Serranidae). Neobenedeniella congeri is 2.13 mm long, 0.83 mm width; and Allobenedenia convoluta is 2.66 mm long, 0.79 m m width, anterior suckers 0.08～0.2×0.04～0.15 mm, marginal hooklets 14 in numb er, 0.009 mm long, egg 0.155×0.128 mm.国家 (863- 819- 0 2 - 0 12 )和福建省自然科学基金 (B9910 0 0 5 )联合资助项

DEVELOPMENT OF MONOGENEAN TREMATODE (NEOBENEDENIA GIRELLAE, MONOGENEA: CAPSALIDAE)

报道了寄生于海水养殖鱼类体表的新本尼登虫 (Neobenedeniagirellae)生活史各期的发育过程。虫卵在 2 5℃～ 2 8℃海水温度条件下 4～ 6d钩毛蚴发育成熟 ;用钩毛蚴人工感染一种鲻科鱼类———棱 (Lizacarinatus)获得成功。在 2 6℃～ 30℃实验室温度条件下 ,钩毛蚴感染棱 10～ 14d后虫体发育成熟 ,并在感染后的第 16天虫体开始产卵。本文对新本尼登虫的生活史发育各期幼虫的形态进行详细的观察、描述、测量和绘图The development of monogenean trematode (Neobenedenia girellae)(Monogenea:Capsalidae), from the maricultured fishes, was studied in Fujian province. All eggs used for this study were obtained from the parasites on the body surface of amberjack fry (Seriola dumerili) collected in floating sea cages, in Xiamen (24°29′N, 118°03′E), Fujian, China. In laboratory, the oncomiracidia hatched from eggs in four to six days at 25℃～28℃ in salt water. Then, the oncomiracidia were used to infect the fish (Liza carinatus) in laboratory. After attaching to the skin of the fish host, the oncomiracidia developed to adult in 10～14 days at the room temperature (26℃～30℃). On the sixteenth day, the adults began to release eggs. The fish host (Liza carinatus) is proved to be a new host record of N. girellae. The morphology of eggs, oncomiracidia, immature worms and adults of this monogenean was described in this paper.国家“8 6 3”计划项目 ( 86 3 819 0 2 0 12 );; 福建省自然科学基金资助项目 (B9910 0 0 5 )~

Study on aetiology of Neobenedenia melleni (Monogenea: Capsalidae) from Marine fishes In Fujian, China

本文对寄生于厦门内海域网箱养殖鱼类———大黄鱼 (Pseudosciaenacrocea)和高体鱼师(Serioladumerili)等名贵经济鱼类体表的单殖类吸虫———梅氏新贝尼登虫(Neobenedeniamelleni)的形态进行观察描述 ,并附简图 .就福建沿海当前流行感染情况及国内外研究概况亦进行了简要介绍 .In 1999, some monogenean ( Neobenedenia melleni ) on the skin of marine fishes ( Pseudosciaena crocea, Seriola dumerili and Epinephelus awoara ) were found in Xiamen, Fujian, China. They were described as follows (Measured in microns). Host: Pseudosciaena crocea , and Seriola dumerili . Locality: Xiamen, Fujian, China. 24°29′ N,118°03′E. Site: Skin and gill. Body elliptical, total length including haptor 2 635～4 689(3 817μm, n =20), maximum width 1 255～2 683(2 015)μm, at anterior end of testes. Haptor almost circular, smooth, shallow, 983～1 081μm×935～1 057(1 051×1 026)μm, aseptate, with 1 pair of accessory sclerites, 1 pair of anterior hamuli and 1 pair of posterior hamuli in linear series and 7 pairs of marginal hooklets. Accessory sclerites stout, scoop shaped, 219～236(228)μm long; anterior hamuli longest, 275～363(318)μm; posterior hamuli short, 125～163(146)μm. Marginal hooklets arranged radially,14 in number. Anterior attachment organs disc suckerlike, each 395～426μm×312～386(413×358)μm. Pharynx glandular, with 5 lobed, 205～359μm×267～446(333×406)μm. Esophagus short. Intestinal crura dendritic medially and laterally, not confluent posteriorly, Four eyes embedded in ganglionic mass. Two testes side by side, spherical, indented, 316～427μm×343～447(375×402)μm. Glands of Goto may be observed. Ovary oval, 355～426μm×338～406(398×370)μm, containing an expanded ventral chamber. Uterus short, leading to common genital pore. Vitelline folliclos, extend anterior to pharynx, posteriorly to posterior end of the body proper. Eggs polyhedral, 128～205μm×143～186(172×165)μm, with a long filament(261～385)μm at the end.福建省自然科学基金!资助项目 (B 9910 0 0 5 ) ;; 国家八六三计划资助!项目 (86 3 819 0 2 0 12

HIRFL-CSR二极磁铁电源的研制；Development of Power Supply for HIRFL-CSR Dipole Magnet

研制了兰州重离子加速器冷却储存环(HIRFL-CSR)二极磁铁电源,提出了一种基于晶闸管相控整流技术和IGBT脉宽调制(PWM)变换技术的同步加速器二极磁铁电源的设计方案,分析、仿真了其工作原理,并设计、生产了1套完整的电源样机。经现场试验、长期运行及测试,电流稳定度<±5×10-5/8h,跟踪精度<±2×10-4,电流纹波<1×10-5。该方案满足HIRFL-CSR二极磁铁对电源技术指标的要求

Effect of Complexes of Phycocyanin and Pine Pollen on Moduating Intestinal Microorganisms

Combining the effects of phycocyanin and pine pollen in promoting intestinal peristalsis and protection，the two compounds were combined to investigate the regulation effect on intestinal microbiota in normal mice. Eighteen 6-8 weeks old C57BL / 6mice were randomly divided into two groups：control group（n=9）and phycocyanin and pine pollen complex group （n = 9）. Before and after interventions，the feces were collected and stored in liquid nitrogen for detection of the micro-organisms of the V3 and V4 hypervariable regions of 16S rRNA. The results showed that compared with the control group，the richness and diversity of the intestinal microbiota in the combination of phycocyanin and pine pollen increased significantly. At the phylum level，the relative abundance of Bacteroidetes increased and the relative abundance of Firmicutes decreased in the phycocyanin and pine pollen complex group （SPC），while the relative abundance of Bacteroidales_S24- 7_group，Lachnospiraceae and Ruminococcaceae increased significantly in the SPC group compared to the control group. However， the relative abundance of Clostridiaceae_1， Clostridiales_vadinBB60 and Coriobacteriaceae decreased significantly. The phycocyanin and pine pollen complex could change the composition and diversity of gut microbes，promote the colonization of beneficial bacteria and reduce the relative abundance of opportunistic pathogens to promote intestinal health