Preparation,identification and preliminary application of monoclonal antibodies against recombinant human basic fibroblast growth factor

目的制备重组人碱性成纤维细胞生长因子(rECOMbInAnT HuMAn bASIC fIbrOblAST grOWTH fACTOr,rHbfgf)单克隆抗体,鉴定其特性,建立双抗体夹心ElISA检测方法。方法以rHbfgf为免疫原,免疫bAlb/C小鼠,通过细胞融合技术建立能稳定分泌抗rHbfgf杂交瘤细胞株,制备抗rHbfgf单克隆抗体,采用Ig亚类ElISA试剂盒鉴定抗体亚类,间接ElISA法检测抗体效,WESTErn blOT鉴定抗体特异性。HrP标记MCAb并建立夹心ElISA检测方法。结果获得2株(分别命名2d3、5f7)可分泌特异性MCAb的强阳性细胞株,腹水抗体效价在10-5以上,Igg亚类均为Igg1,轻链为k链。WESTErn blOT证明2株MCAb特异性良好,双抗体夹心ElISA检测rHbfgf最低检测限达到2 ng/Ml。结论成功制备高效价的抗rHbfgf单克隆抗体,建立抗rHbfgf双抗体夹心ElISA定量检测方法。This study aimed to prepare and identify monoclonal antibodies against recombinant human basic fibroblast growth factor,which will be used in the establishment of a sandwich ELISA method.Balb/c mice were immunized with rhbFGF,and then positive clones of McAbs were obtained after cell fusion and hybridoma selection.The ascites titers,the subclasses type,and the specificity of McAbs were tested by Ig subtype ELISA kit,indirect ELISA method and Western blot analysis,respectively.Moreover,optimization of the sandwich enzyme-linked immunosorbent assay was studied.As results,two of positive hybridomas were screened out,named 2D3 and 5F7 whose ascites titers of McAbs were over 1:105.It proved that the Ig subtypes of 2D3 and 5F7 McAbs were IgG1,and the light chain was kappa.Western bolt identification suggested that either McAbs of 2D3 or 5F7 could react specifically with rhbFGF.The results indicated that two of high titer,specific McAbs against rhbFGF are achieved and can applied to the development of a quantitative double-antibody sandwich ELISA method to detect rhbFGF.温州医学院科研基金(项目5010

吩噻嗪的提纯工艺研究

本文以二苯胺和硫磺为原料,碘催化合成吩噻嗪,改进了提纯工艺。首先,活性炭加热回流脱色。其次,用甲苯在室温下搅拌2.5 h,过滤,收集滤饼,此操作进行两次。在优化的条件下,即m(活性炭)=m(粗产物)*6.25%,m(甲苯)≈m(粗产物)*5.2,得到淡黄色产物。DSC检测熔点合格,并利用MS确认了最终产物。该提纯工艺操作简单,有效的降低了生产成本

Electrochemical Preparation of Fe Core /Pt Shell Nanoparticle Modified Glassy Carbon Electrode and Their Electrocatalytic Activities for Nitrite Reduction

通讯联系人: 陈声培，男，教授; E-mail: shpchen@ xmu． edu． cn; 研究方向:电化学催化、有机电合成、谱学电化学、表面电化学等[中文文摘]通过循环伏安法(CV)在玻碳(GC)电极表面电沉积出分布较为均匀的纳米Fe粒子，制得纳米Fe粒 子修饰的GC(纳米Fe /GC)电极，再经“电荷置换”制得具有Fe核Pt壳结构的纳米粒子修饰的(纳米PtFe / GC) 电极。SEM 结果显示，纳米Fe/GC和纳米PtFe/GC 表面粒子的形貌均呈立方体形，分布较为均匀，粒径在 60 nm 左右。纳米PtFe /GC 电极对亚硝酸盐的还原具有很高的电催化活性。3 种电极的电催化活性顺序依次 为: 纳米Fe /GC ＜ 纳米Pt /GC ＜ 纳米PtFe /GC。相对于纳米Pt /GC 电极，纳米PtFe /GC 电极的起始还原电位 (Ei)正移了0.14V，还原峰电流(ip)增大了3倍。 [英文文摘]Iron nanoparticles were uniformly deposited on glassy carbon electrode by electrochemical cyclic voltammetry，and Fe core/Pt shell nanoparticles ( nano-PtFe) were further obtained through “galvanic replacement”．SEM observation illustrated that cuboid nanoparticles with average sizes，ca． 60 nm，dispersing uniformly on GC electrode surfaces． The electrocatalytical activities for nitrite reduction by nano-Fe /GC， nano-Pt /GC and nano-PtFe /GC electrodes were studied by cyclic voltammetry( CV)． The catalytic activity is in the order of: nano-Fe /GC ＜ nano-Pt /GC ＜ nano-PtFe /GC． The onset potential( Ei ) of nitrite reduction on nano-PtFe /GC electrode is positively shifted 0. 14 V，and the steady reduction current density(ip) is about 3 times of that from nano-Pt /GC electrode．国家自然科学基金(20833005，20873113)、浙江省重中之重学科开放基金(20090503)资助项

抗氧剂壬基二苯胺的制备及性能研究

以自制的选择性固体酸为催化剂,采用二苯胺和1-壬烯为原料制备了壬基二苯胺抗氧剂(NDPA),并研究了其合成工艺。结果表明:通过选择不同的固体酸催化剂,在最优工艺条件下,二苯胺的转化率达99.60%,单取代壬基二苯胺(M-NDPA)的质量分数为30.35%。使用压力差式扫描量热法测定了壬基二苯胺的抗氧化性,结果表明所合成的产物在Ⅰ类基础油150SN、Ⅱ类基础油150N和Ⅳ类基础油PAO中均具有很好的耐受性。使用热重分析(TG)测试了该产物的热稳定性,结果表明该抗氧剂具有优异的热稳定性能

Kinetics of oxygen reduction over Sm05Sr05CoO3 supported on La09Sr01Ga08Mg02O3

Sm0.5Sr0.5CoO3 (SSC) porous electrodes supported on La0.9Sr0.1Ga0.8Mg0.2O3 were studied using X-ray diffraction, scanning electron microscopy, impedance spectroscopy, and dc polarization. The addition of La0.8Sr0.2Ga0.8Mg0.15Co0.05O3 (LSGMC5) or La0.8Sr0.2Ga0.8Mg0.09Co0.11O3 (LSGMC11) into SSC improved its performance significantly. Two arcs could be identified in the impedance spectra of oxygen reduction over SSC electrodes. The conductivities of the high-frequency arcs had no dependency on oxygen partial pressures (p(O2)), which could be related to the transport of oxygen ions. While those of the low-frequency arcs had a p(O2) dependency of about 0.5 at 1073 K, corresponding to a rate-determining step of the diffusion of oxygen atoms at the surface of the electrodes. DC polarization curves of SSC electrodes agreed with the well-known Butler-Volmer equation. Both the cathodic and anodic charge-transfer coefficients were about 1, and the dependencies of the exchange current densities on p(O2) were about 0.25, suggesting a rate-determining step of charge transfer. It seemed that the mechanism of oxygen reduction on SSC electrodes depended strongly on reaction conditions. (C) 2004 The Electrochemical Society

吩噻嗪和-或其衍生物的合成方法及应用

本发明公开了一种吩噻嗪和-或其衍生物的合成方法，其包括：将二苯胺和-或二苯胺衍生物、硫磺和固体酸催化剂混合后置于保护性气氛中充分反应，再经后处理而获得吩噻嗪和-或吩噻嗪衍生物。本发明还公开了所述吩噻嗪和-或其衍生物的用途。本发明提供的吩噻嗪和-或其衍生物的合成方法是一种安全节能、绿色环保的工艺，其工艺操作简单，条件温和，反应周期短，便于控制，工艺过程安全性高，生产过程能耗低，产率高，产品纯度高，反应过程中无需添加水、有机溶剂等，后处理简单，另外原料及固体酸催化剂等来源广泛，廉价易得，并且固体酸催化剂还可回收重复利用，成本低，适于规模化生产

一种酚胺类抗氧剂的合成及性能

以2,6-二叔丁基苯酚和4,4'-二异辛基二苯胺(ODA)为原料,合成了抗氧剂4-({双[4-(1,1,3,3-四甲基丁基)苯基]氨基}甲基)-2,6-二叔丁基苯酚(NBHBODA)。产物结构经NMR和高分辨质谱表征,并对其抗氧化性能和热稳定性进行了测试;对其复配效应进行了考察,对其抗氧化机理进行了推测。结果表明:当质量分数为0.25%的抗氧剂NBHBODA添加到基础油150SN(Ⅰ类)、150N(Ⅱ类)及PAO(Ⅳ类)后,相对于纯油,其氧化诱导期分别提高了7.89倍、5.74倍及8.00倍。抗氧剂NBHBODA的起始分解温度T0.5(失重0.5%时的温度)和终止分解温度T99.5(失重99.5%时的温度)分别为200℃和593℃,与单体ODA及2,6-二叔丁基对甲酚(BHT)相比,起始分解温度分别提高了29℃和131℃,终止分解温度分别提高了308℃和443℃。抗氧剂NBHBODA和T209、含钼化合物3101M复配使用时均表现出优良的协同抗氧化作用,同时添加w(T209)=1.00%和w(NBHBODA)=0.25%的150N氧化诱导期(OIT)的理论值为75.74 min,实测值为118.45 min;同时添加w(NBHBODA)=0.25%和w(3101M)=1.00%的150N氧化诱导期理论值为23.75 min,实测为62.03 min

空间位阻苯酚烷基酯及其制备方法与应用

本发明公开了一种空间位阻苯酚烷基酯、其制备方法与应用。该制备方法包括：在复合碱催化剂存在的条件下，使烷基丙烯酸酯与烷基苯酚化合物反应，再加入高分子量醇类化合物进行酯交换反应，生成位阻苯酚烷基酯化合物，而后用含水无机酸与有机酸组成的混合酸中和任何的催化剂残留物，以生成沉淀的盐和-或油溶性有机酸盐，然后分离出沉淀的盐，获得空间位阻苯酚烷基酯。本发明在位阻苯酚酯的制备过程中，采用复合碱催化剂提高了催化效率，并采用一釜反应方式简化了操作过程，缩短了反应时间，同时还利用混合酸中和的方法简化了催化剂中和及除去过程，降低了废物排放，提高了产品产率和纯度，所获产品可广泛应用于润滑油等产品中

酚胺类抗氧剂、其制备方法及应用

本发明公开了一种酚胺类抗氧剂，其包含具有下式所示结构的化合物：其中，R1、R2和R3分别独立地选自H、烷基、环烷基、芳基、烷芳基及芳烷基；R4和R5中的至少一者选自下式所示结构：其中，R6至少选自-S-、-O-、-NH-，m为0或1；R7和R8分别独立地选自H、烷基、环烷基、芳基、烷芳基及芳烷基；n选自1～12中的任一整数。本发明还公布了一种制备所述酚胺类抗氧剂的方法。本发明的酚胺类抗氧剂具有优异的热稳定性和良好的抗氧化性能，并且其制备工艺简单易行，反应条件温和迅速，后处理简便，该酚胺类抗氧剂适于在润滑油、燃料(特别是燃油)、塑料或橡胶等领域广泛应用