In-situ Photochemical Fluorimetry of Tyrosine and Its Determination

在氨性缓冲溶液中，酪氨酸经２７５ｎｍ紫外光照射后，产生荧光二聚体。据此，建立了现场光化学荧光法测定酪氨酸的新方法。线性范围为 ２．０ × １０－７～ ２．０ × １０－５ ｍｏｌ／Ｌ。检测限及精密度分别为 ３．２ × １０－９ｍｏｌ／Ｌ－１和 １．７％。方法简便、快捷，对其它氨基酸有较好的选择性，可用于啤酒及氨基酸注射液的测定。文章对光化学反应机理作了初步探讨。Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4CI buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2. 0 x 10-7 - 2. 0 x 10-5 mol/L tyrosine. The detection limit is 3 .2 x 10-9 mol/L and relative standard deviation is 1 .7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.福建省自然科学基金资助项目

In-situ photochemical fluorimetry of tyrosine and its determination

Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 x 10(-7) similar to 2.0 x 10(-5) mol/L tyrosine. The detection limit is 3.2 x 10(-9) mol/L and relative standard deviation is 1.7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed