Electro-osmotic Micropump on Glass-based Microfluidic Chip

微流控芯片是以微米级通道网络为基本特征，以微机电系统（MEMS）为加工平台构建的微分析单元与系统，将生物、化学等领域所涉及的样品制备、分离、检测、反应等基本操作单元集成到一块几个平方厘米的芯片上。由于其具有微型化、集成化、自动化、分析速度快、样品消耗少等优点，具有广泛的应用前景，在疾病诊断、药物筛选、环境保护、司法鉴定等领域发挥重要作用。 微流控芯片可以在野外及其他实验室以外的场所使用，因而又被称为芯片实验室。但是，在实际应用中，真正能在野外使用的芯片还相当少见，因为芯片往往需要配备比较复杂的系统进行溶液的驱动和控制等操作。利用电渗现象进行微流体的驱动，可以非常灵巧，便于集成和多路控制，因此...Microfluidic chip is a micro analysis system based on microscale network channel structure and machining platform of MEMS, which integrates the operation units of sample preparation, separation, detection, reaction onto a centimeter-scale chip. Microfluidic chip has been found widespread application prospect in many fields including clinical diagnostics, drug screening, environmental protection an...学位：工学硕士院系专业：化学化工学院化学系_应用化学学号：2052006115202

Mixing Effect of Two-Dimensional Micromixers Based on the Deformed Square-Wave

设计并制作了多种二维变形方波微混合器,通过荧光观测及标准偏差数值分析,在低rE数(rE≤13.33)时,考察拐角和单元长度对混合器混合效果的影响。结果表明,集成拐角的混合器存在一个临界rE值1.3,当rE1.3时,可以产生回流,混合效率随着拐角的减小逐渐提高。对于集成6个单元、拐角为45°、单元长度(S)为3132μM的微混合器,在雷诺系数rE=13.33时,混合效率为56%,约提高了1倍;但当拐角与混合单元长度增大时,混合效率明显降低。A series of two-dimensional micromixers based on the deformed square-wave were designed and fabricated.The effect of the turning angle and unit length on the mixing efficiency at low Reynolds number(Re≤13.33)was investigated by fluorescence observation and numerical analysis of the standard deviation from the pixel intensity distribution.The results indicate that the mixer with the integrated turning angle has a critical Reynolds value of 1.3.The mixture only depends on the molecular diffusion and the mixing efficiencies remained at about 28% when Re1.3.For the micromixer of six square-wave units with 45° turning angle and 3 132 μm unit length,the mixing efficiency is increased to 56% at Re=13.33.However,the mixing efficiency is decreased with turning angle and unit length increasing.国家基础科学人才培养基金资助项目(J0630429);福建省自然科学基金计划项目(D0740013);集美大学科研基金资助项目(ZQ2007001

Y-shaped electric -field free electro -osmotic pump with small sub -channels in parallel

设计并制作了一种y型无电场电渗泵芯片,以聚电解质静电自组装技术在侧臂通道分别修饰正、负电荷形成电渗泵,实现中间主通道无电场干扰。侧臂由多个平行亚通道构成,以增强电渗泵流速。使用中性离子示踪法、毛细管法分别测定电渗泵流速与压强,考察了电场、亚通道个数及深度对流速与压强的影响。结果表明,流速、压强随外加电场增大而增大,并呈线性关系;流速随侧臂亚通道个数增大而增大,压强随通道深度减小而增大。当电场强度为600V/CM时,含9个深10μM、宽度25μM亚通道的电渗泵流速与压强分别为672nl/MIn和442PA。A novel Y-shaped electro-osmotic pump consisting of two arms which has small sub-channels in parallel was developed.Two arm-channels were modified with cationic and anio-nic polyelectrolyte respectively,allowing a field-free flow to be generated in main-channel.Flow rate and pressure were measured by particle tracking and capillary method respectively,and the influence of electric field, numbers and depth of sub-channel on flow rate and pressure was investigated as well.Results showed that both of flow rate and pumping pressure increased linearly with electric field.As the number of subchannels increases,the flow rate becomes higher by increasing the cross-sectional area at a constant electric field.Meanwhile,the pressure falls as depth of sub-channel increases.Flow rate and pumping pressure were 672nL/min and 442Pa respectively for the pump with 9 sub-channels(10am deep、25μm wide)in parallel,when electric field was 600V/cm.国家自然科学基金面上项目20675066;国家基础科学人才培养基金J0630429项目资