Surface Modification and Application of PDMS Microfluidic System

微流控芯片是一个高度多学科交叉的综合性研究领域。从物理化学角度看，由于其单元尺寸都在亚毫米到微米之间，在这个尺度范围内的物理化学规律将发生显著变化,表现为表面现象占了主导因素。因此由于表面的重要性，表面修饰是揭示这个特殊世界的重要手段和有力工具。 本论文工作主要的着眼点在于从物理化学角度出发，利用微系统特殊表面现象，通过表面修饰，进行芯片实验室单元操作的新技术、新方法的探索性研究，具体研究内容如下： 1.通过降低对真空度的要求，建立了一种中真空下PDMS氧等离子体活化及键合的方法。考察了活化后的PDMS基片表面亲疏水性及微通道电渗流的变化。经中真空氧等离子体处理的PDMS基片贴合后可以实现...Microfluidic chip is an interdisciplinary field. From the point of view of physical chemistry, one of the prominent characters is that surface phenomena will dominate many performances in micro-system, because the unit scale in microfluidic chip is from sub-millimeter to micrometer. Therefore surface modification is very important, it is a significant tool and means to discover this special micro-...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：20012505

Surface Modification and Bonding of Poly (dimethylsiloxane) by Oxygen Plasma Treatment under Medium Vacuum

键合是微流控芯片制作的关键技术之一.目前广泛应用的PDMS微流控芯片一般通过高真空(压力低于10 Pa)氧等离子体活化及键合进行制备,需要昂贵的分子泵等设备.通过工艺改进,使用装配普通油泵的等离子体去胶机,在中真空(27 Pa)成功进行聚二甲基硅氧烷(PDMS)表面改性及键合.处理后的PDMS表面亲水性得到极大改善,贴合后可永久性密封,制作微流控芯片.使用扫描电镜,红外光谱及接触角测量仪进行了表征.与文献报道的高真空氧等离子体处理方法相比,效果基本一致,却大幅度降低了对设备系统的要求,并缩短了操作时间.Bonding is one of key processes for fabrication of microfluidic chip.Normally poly(dimethylsiloxane)(PDMS) replica,the most popular microfluidic chip material,were bonded after activated by oxygen plasma in high vacuum(pressure lower than 10 Pa),which need expensive pumps.Here a method was developed for modifying and bonding PDMS replicas in middle vacuum(pressure higher than 13.3 Pa) by oxygen plasma.After treated by oxygen plasma 2~70 s in a system equipped with a normal oil pump(background vacuum 27 Pa),the PDMS replicas were irreversibly sealed when the treated surfaces were brought into touch.Operation parameters,irritation voltage and treatment time,were optimized.And the hydrophilic behavior of PDMS was improved greatly after activating and then decreased slowly to hydrophobic as same as before treatment,but could be kept for a long time by immersed into water immediately after bonding.Compared with the reported procedures using oxygen plasma pretreatment under high vacuum,the presented approach needs simpler and cheaper equipment,and has a higher efficiency.And the bonded chip has same intensity and surface properties,which was proved by FTIR,TEM and contact angle measurement.Other polymer microfluidic replica,e.g.,PMMA,PET,PC etc could be activated and sealed by this method too.福建省科技重点项目(2003H86)资

异源精子在银鲫雌核发育子代中的生物学效应

黑龙江省方正县双凤水库的两性型银鲫群体是三倍体雌核发育种群。异源精子不仅能刺激银鲫卵雌核发育,而且还能影响雌核发育子代的某些性状,如对于子代的生长、性比、体色和肝脏LDH同工酶等都产生了影响。为区别于原有术语“雌核发育gynogenesis”,我们把这种表现了异源精子生物学效应的雌核发育称之为“异精雌核发育allogynogenesis”,发育的子代称之为“异育银鲫”。异育银鲫已以其明显的生长优势在生产上显示了优良的经济性状