东方肉穗草异鼠李素诱导HepG2细胞凋亡研究

目的研究东方肉穗草中的主要抗癌活性成分之一异鼠李素诱导HepG2细胞凋亡的机理。方法采用MTT法检测异鼠李素对肝癌HepG2细胞的抑制作用,DAPI染色法对细胞凋亡进行形态学检测。结果实验结果显示异鼠李素对肝癌HepG2细胞的增殖有明显抑制作用,呈浓度依赖性效应。异鼠李素处理48h的IC50为60μmol/L。荧光显微形态学检测显示异鼠李素处理HepG2细胞后出现细胞凋亡的特征性变化。结论研究表明东方肉穗草异鼠李素可以诱导肝癌HepG2细胞凋亡

Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene

基于慢性粒细胞白血病中BCR/ABL融合基因的碱基序列，设计了一种新型发夹结构锁核酸(locked nucleic acids, LNA)探针，把LNA探针通过Au-s键固定在金电极表面构建了特异的生物传感器.LNA探针与目标链DNA杂交，以自行合成的苯甲酸二聚铜配合物([Cu2(C7H5O2)4(C2H6O)2], 简称[Cu(R)2]2+)为杂交指示剂，应用差示脉冲伏安法进行检测，表现出良好的响应信号.该新型锁核酸传感器能较好的区分完全互补链DNA、单碱基错配链DNA.对互补链DNA检测的线性范围为1.0×10-8～1.0×10-6 mol•L-1，检出限为2.0×10-9 mol•L-1.In this article, a new kind of structure hairpin LNA probe was designed to detect BCR/ABL fusion gene in Chronic Myelocytic Leukemia. The LNA probe was immobilized on the gold electrode(AuE) through sulfur–Au interaction to construct specific electrochemical biosensor. The electrochemical response of the sensor to hybridization of the LNA probe with the target DNA was studied using [Cu(R)2]2+ as an electrochemical indicator. The optimal condition was discussed. The experimental results indicated that in pH 7.4 PBS buffer solution, this new method has excellent specificity for single base mismatch and complementary after hybridization. The relationship between the increased oxidation peak current of [Cu(R)2]2+ and the concentration of complementary strand was linear in the range of 1.0×10-8~1.0×10-6 mol/L. The detection limit was 2.0×10-9 mol/L.国家自然科学基金项目(20675015)，国家863计划(No. 2008AA02Z433)，福建省自然科学基金(No.2010J06011, 2010J01032)，福建省教育厅（No.JA10155），福建省高校产学研科技重点项目(2010Y4003)作者联系地址：福建医科大学药学院，福建 福州 350004Author's Address: College of Pharmacy, Fujian Medical University, Fuzhou 350004,China通讯作者E-mail：[email protected]