The Investigation of Interfacial Hybridization and Functional Nanostructured Biointerface

摘要 界面杂交与功能纳米结构生物体系的研究 近年来，DNA界面杂交模型与功能纳米结构生物体系的构筑，及其界面生物电化学研究已成为生物传感器和生物芯片研究的热点之一。在基因生物传感器的研究中，由于粒子－界面相互作用、界面浓度梯度及位阻现象等原因，使得该固－液相界面反应的热力学及动力学性质与溶液相的相比存在较大的差异。因而，研究固－液界面上的DNA杂交对基因生物传感器的设计具有重要的意义。同时，生物分子与具有及独特光学、电子学、磁学或催化性质的半导体纳米结构材料相结合，可构建功能化纳米结构生物体系，该体系在生物学研究和医学诊断中具有诱人的应用前景；研究生物分子与半导体纳米材料的相互作用...Abstract The Investigation of Interfacial Hybridization and Functional Nanostructured Biointerface Recently，the research interests in the biosensors and biochips are focused on the fabrication of functional nanostructured biointerfaces and model of DNA interfacial hybridization, and the fundamental understanding of the interfacial bioelectrochemical processes within these systems. Thermodynam...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：20042514

DNA/CdS纳米粒子复合体系的光谱和光电化学性质

在水溶液中以DNA作为模板和稳定剂,构筑了DNA与CdS纳米粒子复合体系(DNA/CdSNPC),研究DNA的含量,单双链等对复合体系光电响应的影响,并综合TEM,UV-Vis,IR和荧光光谱等对其形貌和光谱性质进行表征.结果表明,CdS纳米粒子(CdSNPs)与DNA链之间主要通过静电作用结合;DNA模板对CdSNPs的禁带宽度没有影响;以DNA模板合成的CdSNPs具有较高的表面态密度,其对CdSNPs的荧光有增强作用,而对光电流响应有抑制作用,并且DNA在复合体系中的含量影响荧光增强和光电流减弱的程度.该复合体系在荧光标记检测和DNA的定量分析方面可能具有应用前景

Spectroscopic and photoelectrochemical properties of DNA/CdS nanoparticle composites

DNA/CdS nanoparticle composites (DNA/CdS NPC) containing single or double strands and different concentrations of DNA were constructed in aqueous solutions. The effect of DNA on the photoelectrochemical properties of DNA/CdS NPC was investigated and the spectroscopic properties of DNA/CdS NPC were characterized by TEM, UV-Vis, IR, and fluorescent spectrometry. The results showed that CdS nanoparticles (CdS NPs) were combined with DNA strands through the electrostatic interaction; DNA templates did not affect the band gap of CdS NPs; DNA-templated CdS NPs had a higher density of surface states than that stabilized by Na4P2O7, which enhanced the photoluminescence (PL) intensity whereas restrained the photocurrent response of CdS NPs. Besides, there was certain dependency of both the increase of PL intensity and the decrease of photocurrent response on DNA concentrations in DNA/CdS NPC. The composites were hopeful for applications in both fluorescent tagged detections and quantitative analysis of DNA

电化学石英晶体微天平研究界面电场对DNA杂交的影响

采用电化学石英晶体微天平,现场监测不同界面电场下完全匹配的靶标DNA和不完全匹配的靶标DNA分别与寡聚核苷酸探针分子杂交的过程.结果表明,电极表面荷正电时DNA表观杂交效率比电极表面荷负电时高,但假阳性比较显著;而电极表面荷负电时能有效地抑制错配杂交.探讨了引入界面电场后探针分子取向和微观作用力对DNA杂交的影响

Visual inertial measurement unit based inertial guide sample estimation method

本发明提出了一种基于惯性引导采样的视觉IMU方向估计方法，即利用IMU的方向估计信息，引导视觉方向估计中匹配点对的采样过程和误匹配点的移除过程。包括基于增益自适应互补滤波器的IMU方向估计、基于尺度旋转不变的特征检测以及基于视觉IMU融合的方向估计三个步骤。本发明方法采用增益自适应互补滤波器，能够在初始迭代过程中剔除明显的误匹配点对，提高了方向估计的准确性；将IMU的姿态估计信息作为初值引入到视觉方向估计中，通过迭代移除误匹配点对，加快了方向估计的进程，有效避免了采用随机初始值带来的运算量大的问题。本发明方法适用性广、鲁棒性好、准确率高，可广泛应用在人体康复训练的动作捕捉过程中

A fourdimensional microscope for single molecule studies in living cells using astigmatic axial resolution enhancement

<p>An important part of life is to react to the environment and adapt to it. All living cells rely on chemical cascades that form signal pathways, from stimulus to transcription events at the DNA. To know more about these pathways we need tools to visualise the signals.</p> <p>Here is outlined developmental and experimental work on a bespoke microscope system primarily for fast single molecule tracing of proteins in living eukaryote cells. Both hardware and software has been developed. Frame rates and exposure times have been pushed to match the speed of freely diffusing particles in living cells. An EMCCD camera with an electronic gain of 300x and a 5 ms exposure time at 200 frames per second enables this.</p> <p>An astigmatic axial resolution enhancement approach has been implemented to allow tracking with high precision of single fluorescent proteins in real-time in three dimensions in a comparatively large volume. The high resolution in three spatial dimensions and one temporal aim to meet these demands. Software to extract the additional axial information has been developed that can determine axial displacement with 38 nm precision.</p> <p>The microscope system has proven it can find and track small clusters of YPet fluorescent protein in <em>E. coli</em> at short exposure times.</p>This thesis is not currently available via ORA

Study on Response Model of N, P, K Fertilizer in Gynostemma pentaphyllum

采用N、P、K三因素二次D饱和最优设计,在陕西安康平利绞股蓝药源基地进行了绞股蓝氮、磷、钾 肥效田间试验,结合试验点的土壤肥力水平,求得了绞股蓝N、P、K的肥效反应主程,提出了绞股蓝不同用药 部位的N、P、K合理配比和肥料用量。绞股蓝地上部分目标年均产量在3 900-4 600 kg／hm2的施肥量为N 158．05～222．36 kg／hm2,P2O51．915～116．10 kg／hm2,K2O104．06～170．134 kg／hm2。绞股蓝地下根茎目 标产量在900～1200 kg／hm2的施肥量为N 102．88～1 89．02 kg／hm2,P2O5 60．98～100．49 kg／hm2,K2O 152． 31～194．34 kg／hm2。分析N、P、K对绞股蓝生长发育和产量的影响作用,结果表明:不同药用部位的需肥特点 虽不相同,但N、P、K均表现为正效应,都能促进绞股蓝产量的提高,但不能过量。对二因素交互作用分析发 现,NP反应为负,NK反应为正,PK反应为正。说明化学肥料必须在一定限度下以合理的比例施用,否则会造 成减产