38 research outputs found

    Utilization of feruloyl esterase and xylanase for the degradation of brewers' spent grain

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    橘青霉以麦糟为唯一碳源培养时,阿魏酸酯酶的最佳发酵时间为60 H,其酶活力可达40.8 Mu/Ml。在PH值为5.0、45℃、料液比1∶30(g∶Ml)条件下,取47.5 u/Ml的木聚糖酶粗酶液15 Ml,加入1.0 g麦糟的乙醇不溶物,反应12 H后,加入40.8 u/Ml的阿魏酸酯酶粗酶液15 Ml再反应12 H,阿魏酸和低聚木糖释放率分别为54.1%和161 Mg/g(麦糟的乙醇不溶物)。实验结果还表明,阿魏酸酯酶与木聚糖酶存在协同作用,能极大提高麦糟中阿魏酸及低聚木糖的释放率,有利于麦糟的降解。The optimal fermentation time of Penicillium citrinum feruloyl esterase(PcFAE)was 60 h and its activity was 40.8 mU/mL when the Brewers' Spent Grain(BSG)was used as the sole carbon source for Penicillium citrinum.One gram of alcohol-insoluble residue(AIR)of Brewers'spent grain(BSG)was processed by the solution of crude xylanase(XG180)(47.5 U/mL,15 mL)for 12 hours at the condition of the material-liquid ratio 1∶30(W/V),pH 5.0 and 45 ℃,respectively.And then the solution of crude PcFAE(40.8 mU/mL)was added and the sample was processed for another 12 h.The maximum release rate of Ferulic acid(FA)was 54.1% and the release amount of xylooligosaccharide(XOS)was 161 mg/g(BSG-AIR),respectively.The results of our experiments showed that the release rate of FA and XOS from BSG-AIR increased significantly as the PcFAE could coordinate with the xylanase and enzymolysis process was also conducive to the degradation of BSG.华侨大学人才启动基金(11BS221);福建省科技重点项目(2011N0020

    合成气乙醇发酵的微生物研究

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    生物质合成气发酵是一种独特的、经济可行的乙醇生产新方法,它包括气化和发酵两个方面,对环境改善和能源供给有积极的意义。在合成气发酵产乙醇的微生物中,Clostridium ljungdahlii和Clostridium carboxidivomns P7最有应用价值。它们利用合成气的途径是wood-ljungdahl途径。文章概述了合成气发酵产乙醇的菌种和培养条件,建立了生长动力学模型,提出了合成气发酵产乙醇工业化过程中存在的问题和应用前景

    芒草稀硫酸水解工艺条件的正交实验

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    芒草含有80%以上的可降解的纤维素和半纤维素,仅含有19%的不被酸降解的苯环化合物,木质素,适于作为生产燃料乙醇的原料。通过对芒草稀酸水解工艺的正交实验研究,探讨了反应时间、硫酸浓度、原料与硫酸的固液比等因素对纤维素、半纤维素降解为葡萄糖、木糖及总糖含量的影响。结果表明:在121℃条件下,对于纤维素,影响葡萄糖产量的主要因素是硫酸浓度;而对于半纤维素,1.5%的硫酸就可使之降解完全;但在酸浓度较高时,会产生葡萄糖、木糖以外的杂质
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