Preventive effect and mechanisms of 3,3-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells

目的:探究3,3'-二吲哚甲烷(dIM)对过氧化氢(H_2O_2)诱导人角质形成细胞(HACAT)氧化应激作用的预防效应及可能机制。方法:体外培养HACAT细胞,用H_2O_2建立氧化应激模型。采用CCk-8法检测不同浓度(1~20μMOl/l)dIM对HACAT细胞生长的抑制作用;流式细胞术检测dIM作用前后细胞内活性氧自由基(rOS)含量的变化;WESTErn blOT检测不同浓度dIM(0、5、10μMOl/l)对HACAT氧化应激相关蛋白核因子(nf-κb)和丝裂原活化蛋白激酶(MAPkS)磷酸化表达水平的影响。结果:成功建立了HACAT氧化应激模型。CCk-8法研究结果显示1-10μMOl/l dIM对HACAT细胞无明显毒性作用(P>0.05);流式细胞术检测结果表明10μMOl/l dIM预处理可有效预防由H_2O_2诱导的HACAT内rOS产生(P0.05).Flow cytometry results indicated that pretreatment with DIM(10 μmol/L) could inhibit the level of intracellular ROS(P<0.05).With increasing concentration of DIM,the levels of p-p38-MAPK,p-JNK and p-NF-κB were significantly depressed.CONCLUSION:DIM could protect HaCaT cells from H_2O_2-induced oxidative stress via suppressing production of ROS levels and down-regulating the expression of NF-κB and members of MAPKs.DIM might be used as an effective drug to treat or reduce oxidative stressmediated injury in skin cells.国家自然科学基金青年基金(81101562); 广东省科技计划项目(2012B060300005); 广东省自然科学基金(S2012010009633

Direct generation of intense extreme ultraviolet supercontinuum with 35 fs, 11 mJ pulses from a femtosecond laser amplifier

We report on the generation of intense extreme ultraviolet (EUV) supercontinuum with photon energies spanning from 35 eV to 50 eV (i. e., supporting an isolated attosecond pulse with a duration of ~271 as) by loosely focusing 35 fs, 11 mJ pulses from a femtosecond laser amplifier into a 10-mm long gas cell filled with krypton gas. The dramatic change of spectral and temporal properties of the driver pulses after passing through the gas cell indicates that propagation effects play a significant role in promoting the generation of the EUV supercontinuum.Comment: 14 pages, 4 figure

Dynamics of wave propagation in nonlinear optics and hydrodynamics

Several significant wave propagation problems in the fields of nonlinear optics and hydrodynamics are studied in this thesis. In optics, the physical model considered is the two-core optical fiber (TCF), which is an essential component of lightwave technology. In hydrodynamics, the motion of a wave packet on the free surface of water of finite depth allowing modulations from two mutually perpendicular and horizontal directions, governed by the famous Davey-Stewartson (DS) equations, is taken into account. The main contributions of this thesis are: In optics, the effects of the intermodal dispersion (IMD) and the birefringence induced effects, both of which always exist in the TCFs, have been ignored in the previous studies of the modulation instability (MI) of continuous waves (CWs) in the TCFs. In this thesis, a detailed analysis of these effects on the MI spectra has been done. It is found that IMD does not seriously affect the MI spectra of the symmetric/antisymmetric CW states, but can significantly modify the MI spectra of the asymmetric CW states. In exploring the birefringence induced effects, a particular class of asymmetric CW states, which admits analytical solutions and has no counterpart in the single-core fibers, is focused on. It is found that the MI spectra of a birefringent TCF in the normal dispersion regime can be distinctively different from those of a zero-birefringence TCF especially for the circular-birefringence TCF. All the findings of MI analysis can be well verified by the wave propagation dynamics. Another contribution of this thesis is that we find the dramatic pulse distortion and even pulse splitting phenomenon due to IMD in TCFs, which is unexpected in many situations, can be effectively suppressed and even avoided by Kerr nonlinearity, which has never been reported in the literatures in the studies of TCFs. In hydrodynamics, DS equations describe the evolution of weakly nonlinear, weakly dispersive wavepackets with slow spanwise dependences on a fluid of finite depth. Generally, DS equations are divided into two types e, i.e. DSI and DSII equations, depending on the specific fluid configurations (fluid depth, wavelength of the water wave, surface tension etc). Due to the importance of DS equations, many exact solutions have been derived by different nonlinear wave methods over the years in the literature. In this thesis, two new exact doubly periodic wave patterns of DS equations are derived by the use of properties of the theta functions, or equivalently, the Jacobi elliptic functions, and the corresponding solitary waves are also deduced in the long wave limits. The new feature of the two wave patterns found is that they can be applied to both DSI and DSII systems at the same time.published_or_final_versionMechanical EngineeringDoctoralDoctor of Philosoph

Fluorescent protein blotting magnetic composite microsphere preparation method

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种荧光蛋白印迹磁性复合微球的制备方法。以细乳液聚合法将以藻蓝蛋白作为模板的分子印迹与磁敏感性和荧光性材料相结合，即得到藻蓝蛋白分子印迹聚合物磁性和荧光性的复合微球。应用本发明细乳液聚合的方法，在分子印迹聚合物微球中复合有磁和荧光双响应材料，对藻蓝蛋白具有特异性识别与自主吸附，选择性好，吸附效率高，速度快，并在外加磁场作用下方便、快速分离，重复使用性能好，且在较宽的pH范围内进行荧光实时检测和荧光成像。同时所得磁性核壳印迹微球形貌规则、粒径均一。本发明具有成本低、操作简便快捷、重现性好等优点，具有广泛的应用前景

一种汞离子印迹聚合物及其方法和应用

本发明属于环境科学与工程和材料科学领域，具体来说是一种基于双硫腙螯合作用的汞离子印迹聚合物及其制备方法。基于汞与双硫腙的螯合作用，以其螯合物作为模板分子，3-氨丙基三乙氧基硅烷作为功能单体，正硅酸乙酯作为交联剂，以溶胶-凝胶法获得汞离子印迹聚合物。本发明所得聚合物对汞离子具有高的吸附容量、良好的传质速率、优异的识别选择性和高的重复利用性，并且抗干扰能力强。该聚合物结合固相萃取技术实现了对环境和生物样品中汞离子的高选择性富集，进而可用于汞离子的分离和去除

A mercury ion imprinted polymer and its method and application

本发明属于环境科学与工程和材料科学领域，具体来说是一种基于双硫腙螯合作用的汞离子印迹聚合物及其制备方法。基于汞与双硫腙的螯合作用，以其螯合物作为模板分子，3 氨丙基三乙氧基硅烷作为功能单体，正硅酸乙酯作为交联剂，以溶胶 凝胶法获得汞离子印迹聚合物。本发明所得聚合物对汞离子具有高的吸附容量、良好的传质速率、优异的识别选择性和高的重复利用性，并且抗干扰能力强。该聚合物结合固相萃取技术实现了对环境和生物样品中汞离子的高选择性富集，进而可用于汞离子的分离和去除

A quantum dot based protein nuclear shell engram microsphere preparation method

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种基于量子点的蛋白质核壳印迹微球的制备方法。用改进的法合成表面带有氨基的二氧化硅纳米粒子，同时合成表面带有羧基的CdTe量子点，将量子点接枝到二氧化硅表面，采用溶胶凝胶法与表面印迹技术在二氧化硅的表面形成蛋白质印迹层，洗脱掉模板得到基于量子点的蛋白质核壳印迹微球。本发明形成蛋白质印迹层后，由于表面吸附作用的存在，使得量子点的荧光减弱至猝灭；洗脱掉模板分子，量子点的荧光恢复和增强，据此可实现对模板分子藻蓝蛋白的高效识别与高灵敏荧光检测。本发明兼具快速、高选择、高灵敏、易操作、低成本等优势，丰富了分子印迹/蛋白质相关研究

A tubular protein blotting catalytic magnetic micro-motor and its preparation and use

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种管状蛋白质印迹催化磁性微马达的制备方法。以多孔模板辅助化学电沉积法，将藻蓝蛋白作为模板分子，用掺杂聚苯乙烯磺酸钠的聚乙撑二氧噻吩作为电化学选择性材料，以镍作为磁导航材料，金属铂作为固体支架和催化双氧水水解的催化剂，形成管状结构；再以多孔模板辅助化学电沉积法得到管状蛋白质印迹催化磁性微马达。本发明将分子印迹技术与催化、磁性、荧光等响应材料相结合，对藻蓝蛋白具有特异性识别与自主吸附能力。磁性微马达具有选择性好、运动速度快、大小可控、工作寿命长、重现性好、环境要求低等优点，并能在外加磁场作用下可控进行运动