关于反避税中公司法人格否认制度的适用问题探讨

本文先简析公司法人格否认制度作用意义,然后从补充课税原则和保障公平竞争方面入手,总结反避税中公司法人格否认制度必要性,进而在适用场景、适用合理性与不可取代性相关方面,详细分析和阐述公司法人格否认制度在反避税中的使用

Expression of Fusion Protein of Parathyroid Hormone and Transferrin N-terminal Half-molecule in Pichia pastoris

利用重叠PCR技术将PTH(parathyroidhormone,甲状旁腺激素)基因与TFN(transferrinN_terminalhalf_molecule,转铁蛋白N端半分子)基因在体外融合,融合基因克隆至真核表达载体pPIC9中,转化毕赤酵母GS115。转化子经甲醇诱导后,融合蛋白得到了表达并分泌到发酵上清液中。经SPSepharoseFF阳离子交换层析、PhenylSepharoseFastFlow疏水层析纯化获得了纯度大于95%的PTH_TFN样品。Westernblot分析及腺苷酸环化酶实验证明融合蛋白中的PTH具有与抗PTH抗体结合能力及刺激腺苷酸环化酶的活性,铁饱和实验证明融合蛋白中的TFN和单独的TFN具有相同铁结合能力。因而TFN可望作为PTH的天然运输载体。The fused gene (PTH_TFN) of parathyroid hormone (PTH) gene and transferring N_terminal half_molecule (TFN) gene was amplified by multiple PCR and inserted into pPIC9 vector. The recombinant plasmid pPIC9_PTH_TFN was transformed into Pichia pastoris GS115 by PEG. After methanol induction, the target protein was expressed in fermentation supernatant at high level.The fused protein PTH_TFN with purity being higher than 95% was finally obtained after purification through two_step chromatography : SP Sepharose Fast Flow and Phenyl Sepharose Fast Flow.Western blot analysis and adenylate cyclase assay proved that the fused protein exhibited the bioactivity to stimulate cAMP synthesis and the ability to bind Fe ~3+ in the Fe ~3+ saturation study as the recombinant TFN did indicating that TFN could be used as the transcellar carrier of PTH.国家高技术研究与发展项目基金资助(No.2004AA215172)。~

implementation and performance analysis of cpu-gpu parallel matrix multiplication

实现ATI平台上的CPU-GPU混合并行DGEMM,采用在GPU和CPU上同时进行计算的方法来提高运算性能。实验结果证明,当矩阵规模较大时,在AMD Phenom II X4 940和ATI FireStream 9270平台上,混合DGEMM性能相对于单独使用GPU平均提升了16%。通过实验验证了混合DGEMM性能、加速比、任务分配比例的估算方法,并探讨了影响混合DGEMM性能的各种因素

基于改进M-ORB的视觉SLAM直接-闭环检测算法

直接法SLAM不在前端提取图像特征点，使得后端无法生成视觉词袋，这导致大部分直接法SLAM无法使用带有词袋模型的闭环检测来消除系统的累积误差。针对此问题，提出一种基于改进M-ORB的视觉SLAM直接-闭环检测算法，生成闭环检测所需的词袋模型，然后采用词频-逆文档频率算法对视觉词典树各个子节点中的视觉单词进行自适应分配权重，得到场景信息的准确表述。在TUM、KITTI两种公开数据集上进行了对比实验，实验结果表明，所提出的算法能够有效检测到闭环，并在不降低准确性的同时，提高SLAM的实时性与鲁棒性

α-酮戊二酸及其钠盐对雨生红球藻生长和虾青素积累的促进作用

雨生红球藻是已知虾青素(astaxanthin,AST)含量最高的生物,是理想的细胞制备工厂。尽管研究表明添加α-酮戊二酸(OG,α-oxoglutarate)能有效促进混养条件下雨生红球藻AST积累,但在自养条件下是否也有类似效果不清楚。研究以自养雨生红球藻为对象,在正常(CK)、高光(HL)、缺氮(DN)和高光-缺氮双重逆境(HL-DN)下从细胞生长和AST积累两方面评估OG及其钠盐(OG-2Na)的促进作用。结果显示,添加OG或OG-2Na显著缓解HL、DN和HL-DN胁迫对细胞生长的抑制作用,培养至6d的生物量分别为0.58、0.53和0.38g/L,约是未添加组的2.0倍。添加OG或OG-2Na显著促进AST积累。培养至6d,在HL、DN和HL-DN下,AST含量分别达到13.62、19.51和28.29mg/g,是未添加组的2.39、1.16和1.35倍。同时在胁迫条件下,添加OG或OG-2Na有效提高单位细胞中AST含量。针对OG和OG-2Na添加,最大单位细胞AST含量分别出现在HL和HL-DN条件下,达到53.72和60.58pg/cell,是对照的3.09和2.83倍。在CK下,添加10.0mg/L的OG或OG-2Na,其AST含量显著高于单独的胁迫条件。上述结果证实OG或OG-2Na不但可减少HL、DN和HL-DN胁迫对红球藻细胞生长的抑制、显著提高其AST含量,是一种在提高AST产量的有效途径

Phenotypic Characterization of Blue Photoreceptor Plant Type Cryptochrome CRY Mutant in Chlamydomonas reinhardtii

The phenotypic differences of Chlamydomonas reinhardtii wild strain(CC5325)and CRY mutant strain(crcry)under normal CK(Control)and Blue BL(Blue light)cultures were investigated. The PCR verification showed that the crcry mutant was inserted with paromomycin resistance gene AphVIII expression box in the coding region. AphVIII resistance gene was inserted into crcry mutant and successfully expressed in plate and liquid culture system with paromomycin as screening condition. Phenotypic identification showed that there were no significant differences in growth, pigment, photosynthesis and lipid synthesis between wild strain CC5325 and mutant strain crcry under CK culture condition. However, under BL condition, the growth of mutant crcry was significantly inhibited, and the color of algae liquid turned yellow. Chlorophyll a, chlorophyll b and total pigment contents of unit cells decreased significantly, while total carotenoid contents increased significantly. The photosynthetic system was severely suppressed and the total lipid content significantly reduced. In conclusion, plant cry is involved in the blue light response of C. reinhardtii

α-酮戊二酸及其钠盐对雨生红球藻生长和虾青素积累的促进作用

雨生红球藻是已知虾青素(astaxanthin,AST)含量最高的生物,是理想的细胞制备工厂。尽管研究表明添加α-酮戊二酸(OG,α-oxoglutarate)能有效促进混养条件下雨生红球藻AST积累,但在自养条件下是否也有类似效果不清楚。研究以自养雨生红球藻为对象,在正常(CK)、高光(HL)、缺氮(DN)和高光-缺氮双重逆境(HL-DN)下从细胞生长和AST积累两方面评估OG及其钠盐(OG-2Na)的促进作用。结果显示,添加OG或OG-2Na显著缓解HL、DN和HL-DN胁迫对细胞生长的抑制作用,培养至6d的生物量分别为0.58、0.53和0.38g/L,约是未添加组的2.0倍。添加OG或OG-2Na显著促进AST积累。培养至6d,在HL、DN和HL-DN下,AST含量分别达到13.62、19.51和28.29mg/g,是未添加组的2.39、1.16和1.35倍。同时在胁迫条件下,添加OG或OG-2Na有效提高单位细胞中AST含量。针对OG和OG-2Na添加,最大单位细胞AST含量分别出现在HL和HL-DN条件下,达到53.72和60.58pg/cell,是对照的3.09和2.83倍。在CK下,添加10.0mg/L的OG或OG-2Na,其AST含量显著高于单独的胁迫条件。上述结果证实OG或OG-2Na不但可减少HL、DN和HL-DN胁迫对红球藻细胞生长的抑制、显著提高其AST含量,是一种在提高AST产量的有效途径