一种用于集成固相萃取柱的微流控芯片

本实用新型涉及微流控芯片，具体地说是一种用于集成固相萃取柱的微流控芯片，芯片由上、下二层基片封接而成，其上层基片设有进样口、出样口及微通道，在微通道上设有微粒引入通道及其入口，在微粒引入通道与进样口和出样口之间分别设有坝结构，坝结构的顶面与下层基片间存有缝隙，二个坝结构形成了一双围堰式结构，二个坝结构之间的微通道形成填充柱床通道。芯片含有一个微粒引入通道，通过该通道可实现固定相微粒的填充和更新。本实用新型提供的用于集成固相萃取柱的微流控芯片制作简便、快速、成本低廉，适用范围广、通用性强。带填

虚拟家居设计系统中基于约束的三维布局

在三维场景中对物体进行布局是一项费时而单调的工作。本文以虚拟家居设计系统为应用背景,分析了虚拟家居设计系统中三维布局操作的需求和特点以及系统中的约束类型,提出了一种基于约束的三维布局算法,旨在提高三维操作的便捷性,减少不合理设计结果。应用实例表明,该算法在虚拟家居设计系统的实际应用中取得了较好的效果,对于设计过程中的虚拟布局有重要借鉴意义

adaptive user interface for dynamic schedule system

该文以炼化企业的动态调度系统为应用背景,以面向动态调度的自适应用户界面的设计与实现为主要研究内容,分析了当前炼化企业动态调度系统用户操作的流程以及界面的需求并建立起相应的数学模型。借鉴自适应用户界面的相关研究成果,设计了自适应用户界面的体系结构以及用户的动态行为过程,针对流程行业的应用特点提出了利用产生式推理和基于上下文的用户意图捕捉的方法,最后给出应用实例。文中所述方法已经成功在企业中得到应用。使用结果表明,该方法能够有效地开发动态调度系统的自适应用户界面,获得满意效果

Feeding habit study of Anchovy(Engraulis japonicus)in the central and southern part of the Huanghai Sea with stable isotope techniques

根据2005年4~5月在黄海和长江口海域进行的春季底拖网调查,应用稳定同位素方法研究了黄海中南部鳀鱼(Engraulis japonicus)及其可能摄食饵料的碳氮稳定同位素比值,结果表明:(1)黄海中南部鳀鱼的食物组成为不同粒径的浮游动物、太平洋磷虾(Euphausia pacifica)和仔稚鱼,其中以粒径为500~900μm的浮游动物为主,贡献比例占61%~84%;仔稚鱼贡献比例占16%~21%,较传统胃含物方法分析的结果小;(2)黄海中部海域鳀鱼的碳氮稳定同位素比值平均值较南部海域高,原因可能与各海域的能量来源不同或存在微食物环有关,也可能与不同海域鳀鱼的能量转换途径不一样,即与食物链长短不一有关.Stable isotope techniques are commonly used to represent the trophic structure of aquatic systems.Based on the bottom trawl surveys in the central and southern part of the Huanghai Sea and adjacent sea area of Changjing River Estuary during the spawning season of Spring in 2005,we mainly focus on the stable carbon(13C/12C) and nitrogen isotope(15N/14N) ratios of anchovy and its potential food,such as zooplankton,Euphausia pacifica,larvae and juvenile fish,Leptochela gracilis,Loligo beka in order to acknowledge the feeding habit of anchovy.Application of δ13C and δ15N in dietary study depends upon the differentiation in the isotopic composition of various food sources.The δ13C and δ15N values of anchovy and its potential food indicated that its food sources in these areas were composed of zooplankton,Euphausia pacifica,fish larvae and juvenile fish.Especially,500~900 μm zooplankton was the most important dietary component for anchovy,accounting for 61%~84% of the total food by weight,the food proportion of the fish larvae and juvenile fish was 16%~21%,which was less than the results with the method of stomach content analysis;the variations in δ13C and δ15N values of anchovy in the central of the Huanghai Sea were higher than the area of southern part of the Huanghai Sea and adjacent sea of Changjing River Estuary,the reason were likely to difference between the sources of Anchovy energy or the length of the food chain in the different areas of the central and southern of Huanghai Sea and adjacent sea area of Changjiang River Estuary.Stable isotope analysis(δ13C and δ15N) can be a powerful tool in both identifying the source of organic matter that supports fish production and investigating food web remains untested using traditional dietary methods in the modern ecology.国家自然科学基金重大项目(30490233);; 国家自然科学基金项目(3037110430570293)资

一种带筛孔的塑料微流控芯片的制备方法

本发明提供了一种带筛孔的塑料微流控芯片的制备方法，包括模版制备、芯片浇注步骤，其中模版制备包括覆膜、图形设计、光刻、化学刻蚀，其特征在于图形设计时，将模版待生成筛孔的位置设计成曲线形状。本发明提供的带筛孔的塑料微流控芯片的制备方法，成品率高、硅片制作省时、省力。带填

多通道微流控芯片

本外观设计产品为一种分离检测用微流控芯片，通体由透明材料制成，为双层板结构；进出口设置于上层板上；微通道刻于上层板的底部，由下层板封接。由于主视图与后视图相同，省略后视图。左视图与右视图相同，省略右视图。俯视图与仰视图相同，省略仰视图。带填

glassmicrofluidicchipbasedsodiumdodecylsulfatenongelsievingelectrophoresisfordeterminingmolecularweightsofimmunoglobulinfragments

The concentrations of dextran sieving matrix and running buffer were optimized. Under the optimized conditions (10% (w/v) dextran, 0.1% SDS, 10% glycerol, 0.2 mol/L Tris-boric acid, pH 8.3), six standard proteins could be efficiently separated using sodium dodecyl sulfate (SDS)-nongel sieving electrophoresis on home-made glass microfluidic chip. The relative standard deviations (RSDs) for migration time were lower than 0.50%. A good linear relationship (r =0.994) was built between the migration time of each protein and the logarithm of its molecular weight (logMr). Using the chip-based electrophoretic separation system, the molecular weights of different fragments of immunoglobulin G after different treatments were measured, and the results matched the facts