Clinical efficacy of the fine needle aspiration of the cell block in the diagnosis of parotid gland masses

目的探讨超声引导下细针穿刺制作细胞块在诊断腮腺区肿块方面的应用价值。方法在彩色超声仪引导下,对285个腮腺区肿块进行细针穿刺,将穿刺标本制成细胞块,以进行病理学诊断。对非肿瘤性肿块采取保守治疗,囊肿与肿瘤性肿块采取手术治疗。对术后病理确诊为腺样囊性癌(ACC)与多形性腺瘤(PA)对应的细胞块行干细胞因子受体CD117免疫组织化学检测。结果标本制作满意率为95.1%(271/285),诊断准确率为94.5%(256/271);诊断敏感度为87.0%(67/77),特异度为98.1%(157/160)。CD117在ACC中的阳性表达率为95.2%(20/21),在PA中为20.3%(25/123),ACC中阳性表达率明显高于PA,二者差异有统计学意义(P<0.01)。结论超声引导下腮腺区肿块细针穿刺制作细胞块结合分子标志物检测,对腮腺区肿块的诊断具有重要意义。Objective To investigate the clinical significance of cell blocks obtained by ultrasound-guided fine needle aspiration in diagnosing parotid gland masses. Methods Cell blocks were made in 285 parotid gland masses by ultrasoundguided fine needle aspiration. Diagnosis was conducted using the cell blocks. Non-tumor masses were subjected to conservative treatment, and cysts and tumors were treated with surgery. The cell block sections from masses with the diagnosis of adenoid cystic carcinoma(ACC) and pleomorphic adenoma(PA) were applied to the detection of immunocytochemical staining for the stem cell factor receptor CD117. Results The satisfaction rate of the specimen was 95.1%(271/285). The accuracy rate of the diagnosis was 94.5%(256/271), the sensitivity was 87.0%(67/77), and the specificity was 98.1%(157/160). The positive rate of CD117 in ACC was 95.2%(20/21), whereas that in PA was 20.3%(25/123). The positive rate of CD117 in ACC was higher than that in PA(P<0.01). Conclusion The use of cell blocks obtained from ultrasound-guided fine needle aspiration, together with molecular marker detection, has great significance in diagnosing parotid gland masses

Novel System of Direct Methanol Fuel Cell Anode Catalysts: NanoTiO_2-CNT-PtNi Complex Catalysts

采用电合成前驱体Ti(OEt)_4直接水解法和电化学扫描电沉积法制备纳米TiO_2-CNT-PtNi复合纳米催化剂.透射电镜(TEM)和X射线衍射(XRD)测试结果表明,纳米PtNi合金粒子(平均粒径8nm)均匀地分散在纳米TiO2-CNT复合膜的三维网络结构中.通过暂态电化学方法研究表明,复合纳米催化剂的电化学活性比表面积为90m~2/g,对甲醇氧化具有很高的电催化活性和稳定性,常温常压下甲醇氧化峰电位为0.67和0.44V,当温度为60℃时,氧化峰电位负移至0.64和0.30V,氧化峰电流密度高达1.38A/cm~2.复合纳米催化剂对甲醇电氧化的高催化活性和稳定性可归因于多元复合纳米组分的协同催化作用,这种作用导致CO在复合纳米催化剂上的弱吸附,从而避免了催化剂的中毒.The nanoTiO2-CNT-PtNi complex catalysts were prepared by the direct hydrolysis of electro-synthetic precursor Ti(OEt)4 and electrochemical scan electrodepositing method. The results of XRD and SEM show that the PtNi nanoparticle of average size 8 nm was dispersed uniformly on nanoTiO2-CNT com-plex film surface. The electrocatalytic activity of the nanoTiO2-CNT-PtNi complex catalysts was investi-gated by cyclic voltammetry and chronopotentiogram. The results indicated that the nanoTiO2-CNT-PtNi complex catalysts with Pt loading of 0.32 mg/cm2 exhibited high electrochemically active surface area of 90 m2/g and very high electrocatalytic activity and stability for electro-oxidation of methanol. The oxidation peak potential of methanol was 0.67 and 0.44 V at room temperature in atmosphere pressure, respectively, and shifted to 0.64 and 0.30 V at 60 ℃ and the oxidation peak current of methanol was 1.38 A/cm2. The high electrocatalytic activity and good stability can be attributed to the synergistic catalytic effect of nano-composite, which leads to the weak adsorption of CO on complex nanostructure catalysts, avoiding poison-ing of the catalysts.国家自然科学基金(No.20476001);; 安徽省自然科学基金(No.00045317)资助项目

透皮促进剂对白花前胡甲素体外经皮渗透的影响

目的:考察透皮促进剂对白花前胡甲素(dl-praeruptorin A,Pd-Ia)体外经皮渗透的影响。方法:采用改进的Franz扩散池,以大鼠离体皮肤为渗透屏障,用高效液相色谱法对Pd-Ia进行含量测定,考察月桂氮酮(Azone)及1%Azone与不同浓度丙二醇(PG)混合物对Pd-Ia透皮吸收的影响。结果:使用Azone对Pd-Ia有促透作用,1%Azone效果较好,平均渗透速率达到4.064μg.cm-2.h-1;1%Azone与15%PG合用促透效果最好,平均渗透速率达到4.889μg.cm-2.h-1,且与单用1%Azone有显著性差异(P<0.05)。结论:1%Azone与15%PG合用时,含0.5%Pd-Ia溶液体外渗透具有最大促透效果,体现出协同作用

Novel system of direct methanol fuel cell anode catalysts: NanoTiO(2)-CNT-PtNi complex catalysts

The nanoTiO(2)-CNT-PtNi complex catalysts were prepared by the direct hydrolysis of electrosynthetic precursor Ti(OEt)(4) and electrochemical scan electrodepositing method. The results of XRD and SEM show that the PtNi nanoparticle of average size 8 nm was dispersed uniformly on nanoTiO(2)-CNT complex film surface. The electrocatalytic activity of the nanoTiO(2)-CNT-PtNi complex catalysts was investigated by cyclic voltammetry and chronopotentiogram. The results indicated that the nanoTiO(2)-CNT-PtNi complex catalysts with Pt loading of 0.32 mg/cm(2) exhibited high electrochemically active surface area of 90 m(2)/g and very high electrocatalytic activity and stability for electro-oxidation of methanol. The oxidation peak potential of methanol was 0.67 and 0.44 V at room temperature in atmosphere pressure, respectively, and shifted to 0.64 and 0.30 V at 60 degrees C and the oxidation peak current of methanol was 1.38 A/cm(2). The high electrocatalytic activity and good stability can be attributed to the synergistic catalytic effect of nanocomposite, which leads to the weak adsorption of CO on complex nanostructure catalysts, avoiding poisoning of the catalysts

Direct generation of intense extreme ultraviolet supercontinuum with 35 fs, 11 mJ pulses from a femtosecond laser amplifier

We report on the generation of intense extreme ultraviolet (EUV) supercontinuum with photon energies spanning from 35 eV to 50 eV (i. e., supporting an isolated attosecond pulse with a duration of ~271 as) by loosely focusing 35 fs, 11 mJ pulses from a femtosecond laser amplifier into a 10-mm long gas cell filled with krypton gas. The dramatic change of spectral and temporal properties of the driver pulses after passing through the gas cell indicates that propagation effects play a significant role in promoting the generation of the EUV supercontinuum.Comment: 14 pages, 4 figure

Fluorescent protein blotting magnetic composite microsphere preparation method

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种荧光蛋白印迹磁性复合微球的制备方法。以细乳液聚合法将以藻蓝蛋白作为模板的分子印迹与磁敏感性和荧光性材料相结合，即得到藻蓝蛋白分子印迹聚合物磁性和荧光性的复合微球。应用本发明细乳液聚合的方法，在分子印迹聚合物微球中复合有磁和荧光双响应材料，对藻蓝蛋白具有特异性识别与自主吸附，选择性好，吸附效率高，速度快，并在外加磁场作用下方便、快速分离，重复使用性能好，且在较宽的pH范围内进行荧光实时检测和荧光成像。同时所得磁性核壳印迹微球形貌规则、粒径均一。本发明具有成本低、操作简便快捷、重现性好等优点，具有广泛的应用前景

一种汞离子印迹聚合物及其方法和应用

本发明属于环境科学与工程和材料科学领域，具体来说是一种基于双硫腙螯合作用的汞离子印迹聚合物及其制备方法。基于汞与双硫腙的螯合作用，以其螯合物作为模板分子，3-氨丙基三乙氧基硅烷作为功能单体，正硅酸乙酯作为交联剂，以溶胶-凝胶法获得汞离子印迹聚合物。本发明所得聚合物对汞离子具有高的吸附容量、良好的传质速率、优异的识别选择性和高的重复利用性，并且抗干扰能力强。该聚合物结合固相萃取技术实现了对环境和生物样品中汞离子的高选择性富集，进而可用于汞离子的分离和去除

A tubular protein blotting catalytic magnetic micro-motor and its preparation and use

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种管状蛋白质印迹催化磁性微马达的制备方法。以多孔模板辅助化学电沉积法，将藻蓝蛋白作为模板分子，用掺杂聚苯乙烯磺酸钠的聚乙撑二氧噻吩作为电化学选择性材料，以镍作为磁导航材料，金属铂作为固体支架和催化双氧水水解的催化剂，形成管状结构；再以多孔模板辅助化学电沉积法得到管状蛋白质印迹催化磁性微马达。本发明将分子印迹技术与催化、磁性、荧光等响应材料相结合，对藻蓝蛋白具有特异性识别与自主吸附能力。磁性微马达具有选择性好、运动速度快、大小可控、工作寿命长、重现性好、环境要求低等优点，并能在外加磁场作用下可控进行运动

A mercury ion imprinted polymer and its method and application

本发明属于环境科学与工程和材料科学领域，具体来说是一种基于双硫腙螯合作用的汞离子印迹聚合物及其制备方法。基于汞与双硫腙的螯合作用，以其螯合物作为模板分子，3 氨丙基三乙氧基硅烷作为功能单体，正硅酸乙酯作为交联剂，以溶胶 凝胶法获得汞离子印迹聚合物。本发明所得聚合物对汞离子具有高的吸附容量、良好的传质速率、优异的识别选择性和高的重复利用性，并且抗干扰能力强。该聚合物结合固相萃取技术实现了对环境和生物样品中汞离子的高选择性富集，进而可用于汞离子的分离和去除

A quantum dot based protein nuclear shell engram microsphere preparation method

本发明属于材料科学与工程和生物分离工程领域，具体来说是一种基于量子点的蛋白质核壳印迹微球的制备方法。用改进的法合成表面带有氨基的二氧化硅纳米粒子，同时合成表面带有羧基的CdTe量子点，将量子点接枝到二氧化硅表面，采用溶胶凝胶法与表面印迹技术在二氧化硅的表面形成蛋白质印迹层，洗脱掉模板得到基于量子点的蛋白质核壳印迹微球。本发明形成蛋白质印迹层后，由于表面吸附作用的存在，使得量子点的荧光减弱至猝灭；洗脱掉模板分子，量子点的荧光恢复和增强，据此可实现对模板分子藻蓝蛋白的高效识别与高灵敏荧光检测。本发明兼具快速、高选择、高灵敏、易操作、低成本等优势，丰富了分子印迹/蛋白质相关研究