Raman Spectroelectrochemical Study on Bioactive Molecules

本文概述了采用电化学现场拉曼光谱技术研究氧化物歧化酶在L 半胱氨酸修饰金电极表面的电子迁移反应以及腺嘌呤共存条件下超氧化物歧化酶在金电极表面的电子迁移反应和不同电位下银电极表面烟酰胺腺嘌呤二核苷酸的吸附等体系的反应吸附特性 .所得结果对于分析和研究生物活性分子电化学过程机理具有重要意义 .Electron?transfer reaction is known to be one of the key reactions for generating biological functions. Mechanism revelation at a molecular level of such kind reactions is to be very helpful for us to understand life essence. In fact, surface enhanced Raman scattering (SERS) is one of the most powerful tools for the study on metal?electrolyte and metal?vacuum interfaces since 1970's. Moreover, Raman spectroscopic study in enzymology has provided attractive results during last twenty?five years. For the study of electron?transfer reaction mechanism of some oxidoreductases and SERS of some other biological macromolecules, an electrochemical in situ Raman spectroscopic technique was established in author's lab and some research works have been done on it in the past two years. A brief review of these works is given in this paper. The electrochemical in situ Raman spectroscopic measurements were carried out using a Super LABRAM Raman spectrometer (Dilor, France) coupled with a CHI604A Electrochemical Analyzer (CH Instr., USA). A Teflon spectroelectrochemical cell with a quartz plate window was designed for the in situ measurements. The working electrode was pretreated with oxidation?reduction cycles for each measurement. The electrolyte solutions were purged with nitrogen prior to all measurements, and all the measurements were carried out under the nitrogen atmosphere. Copper, zinc superoxide dismutase (SOD) is an important oxidoreductase for organism metabolism. The established spectroelectrochemical technique was first used to characterize the cyclic voltammetric process of SOD at L?cysteine modified gold electrode as well as the process of electrochemical modification of L?cysteine molecules on a gold electrode. The obtained Raman spectra reveal that the L?cysteine modified gold electrode improves effectively the reversibility of electron?transfer reactions of SOD. Besides L?cysteine molecules, it was interesting that adenine was also an effective electron?transfer promoter for SOD at gold electrode. A strong peak at 355 cm -1 can be observed in the Raman spectrum of adenine molecules adsorbed on gold electrode. It was inferred that the peak maybe related to the chemical interaction between adenine molecules adsorbed and gold electrode surface. As shown in Fig.1, for the mixture of SOD and adenine at gold electrode under a polarization potential 55 mV (vs. SCE), both the characteristic Raman lines of SOD and adenine molecules appeared. Therefore it was reasonable to conclude that SOD and adenine molecules should be co?adsorbed on gold electrode surface under such a potential, which is slightly lower than the reduction peak potential of SOD on adenine?modified gold electrode. Moreover, two new peaks appeared remarkably at 445 cm -1 and 610 cm -1 are likely to be related to the active site of SOD. It suggests that the co?adsorption mechanism of SOD and adenine molecules on the gold electrode surface results in effective approaching of the active site of SOD to the electrode surface.作者联系地址：上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234Author's Address: Dept. of Chem., Shanghai Teachers Univ., Shanghai 200234,Chin

内蒙古锡林河湿地物种多度分布格局

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A Study on the Pattern of Wetland Species Abandance Distribution in Xilin River

物种多度分布是研究物种多样性分布的重要组分。以Lognormal模型、Logseries模型和Weibull模型、Exp模型、Power模型来拟和内蒙古锡林河漫滩典型草甸:无脉苔草(Carex enervis)、针苔草(Carex dahurica)、荸荠(Eleocharis intersita)、长叶火绒草(Leontopodium longifolium)、箭叶橐吾(Ligularia sagitta)和密花凤毛菊(Saus-sures acuminata)群落和塔头草甸(包括塔头和塔头间群落)的物种多度分布格局。比较群落整体的物种多度分布和区分成常见种、偶然种及中间种之后的多度分布特征。结果表明,在log-相对多度-物种级数这个水平上,5个模型对于整个群落物种多度分布均不能很好的拟和;但是按照常见种等细化后,Weibull、Power和Log-seris模型分别对典型草甸群落常见种、偶然种和中间种能很好的拟和,而Logseries和Power模型对于塔头群落的常见种和偶然种拟和较好。然而,在物种-游程这个水平上,5个模型则能较好地拟和整个群落物种多度;在区分常见种和偶然种之后,拟合结果更优,且典型群落和塔头群落一致,即Lognormal模型对所有种拟和是最好的,而Power模型对偶然种的拟和是最好的。不同的拟和模型应用于不同的群落类型,可以看出湿地群落的复杂性和生境的多样性。典型草甸群落和塔头群落尽管在表现形式上不同,但是群落的内部仍存在相似的联系,可能跟相似物种的作用有关。

高电压型纳米Al_2O_3包覆LiNi_(1/3)Co_(1/3)Mn_(1/3)O_2的制备和电化学性能

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Preparation method and microcapsule of visceral oligopeptide microcapsule of ginseng ginseng

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The utility model relates to a sea cucumber viscera intestinal sand separating device

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The utility model relates to a Chinese herbal medicine glycolysis extracting device with homology of medicine and food

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Lactobacillus plantarum LHP710 for producing gamma-aminobutyric acid and application thereof

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