Expression of pin1 gene in lung cancer and its significance

背景与目的:Pin是人类高度保守的特异性磷酸化肽基脯氨酰顺及异构酶,它作用于脯氨酸所形成的肽键,并且仅使磷酸化pSer/Thr-Pro发生异构化,这一磷酸化后调控机制能诱导磷酸蛋白的构像变化,使其发挥功能。近来的研究显示这种新的调控机制在许多生理过程中起重要的调节作用,一旦失调便导致一系列人类疾病。如癌症患者体内Pin1表达异常增高,并调控多种癌基因的信号通路。本研究探讨pin1基因在非小细胞肺癌(NSCLC)中的表达及其意义。方法:利用免疫组织化学方法、定量PCR方法分别检测肺癌组织和正常肺组织中Pin1基因在翻译水平和mRNA水平上的表达差异。结果:在蛋白质水平上,Pin1的表达在正常/癌两种不同的肺组织中,表达量差异有显著性;而在mRNA水平上,Pin1的表达量在正常/癌两种不同的肺组织中,差异无显著性。结论:Pin1在NSCLC组织中的表达,可能是在翻译水平受到了调控;我们的研究进一步证实:Pin1在NSCLC组织中存在蛋白水平的过量表达,这对利用Pin1作为NSCLC组织检测标志物提供了理论依据。Background and purpose:Pin1 is a highly conservative enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Recent results indicate that such conformational changes following phosphorylation are a novel signaling mechanism pivotal in regulating many cellular functions. Overexpression of Pin1 is prevalently found in human cancers.We studied expression of pin1 in non-small cell lung cancer and its implication in clinical. Methods:We detected the differential expression level of Pin1 protein in lung cancer specimen and normal lung tissues by immunohistochemical staining. Real-time quantitative PCR was also applied to detect the mRNA differential expression level in lung cancer tissues and normal lung tissues. Results:Pin1 protein is overexpressed in lung cancer at the protein level. On the other hand, the expression level of Pin1 mRNA in lung cancer tissue has no significant change compared with normal tissue. Conclusion:It demonstrated that the Pin1 expression in lung cancer might be regulated by translation mechanism.Our data implicate that Pin1 may serve as a valuable molecular marker for human cancer

First record of genera Rutilaria and Trigonium(Bacillariophyta) and 11 diatom taxa(species and varieties) in South China Sea

Diatom taxa in samples collected from the uppermost 1-cm sediment of the South China Sea(SCS) during cruises in 2000,2001,and 2007 were investigated.Among them,two genera,Rutilaria and Trigonium,and 11 marine taxa(species and varieties) were described for the first time in China.The 11 taxa,Rutilaria radiata,Asterolampra grevillei,Biddulphia turrigera,Cocconeis cyclophora var.decora,Cocconeis ocellata,Dictyoneis marginata,Entogonia davyana,Tryblionella campechiana,Plagiogramma kinkeri,Plagiogramma nankoorense and Trigonium cf.contumax,were mainly extant species,although Rutilaria radiata and Entogonia davyana are fossils.Available data show that these species are mainly present in the eastern and western Pacific Ocean and the Indian Ocean.A large number of taxa newly recorded in this study were previously reported in the Philippines.The presence of these taxa in both locations is likely a result of seawater exchange in the SCS through the Bashi Channel.Taxonomic descriptions,habitats,and distributions of each..

Numerical simulation of gaseous detonation waves propagation and comparison with experimental results

本文应用基元反应模型和频散可控耗散格式(DCD)对氢氧爆轰波进行了二维数值模拟.氢氧混合物的化学反应模型考虑了8种组分20个反应方程式.在处理化学反应引起的刚性问题时采用了时间算子分裂的方法.本文首先对爆轰波数值结果和实验结果进行了对照验证,然后对爆轰波在楔面反射由马赫反射向规则反射转变的过程进行了数值分析,得到了反射转变临界角,并和实验结果及理论分析进行了比较,结果是令人满意的;本文还对爆轰波的多波结构进行了初步的数值分析

Time-frequency filtering method for detecting clicks of bottlenose dolphin (Tursiops truncates)

针对宽吻海豚Click信号检测提出了一种在信号时频图中基于Gabor滤波器的检测方法。该方法首先对声信号进行分段处理,计算每一段信号的时频图;然; 后设计Gabor滤波器,提取时频图中垂直方向的线条;对Gabor滤波处理后的时频图进行自适应阈值处理,提取时频图中能量较强的区域;最后通过连通域; 分析确定Click信号的位置。仿真合成不同信噪比的测试信号,本文算法在Click信号和背景噪声平均功率比为15; dB的情况下,Click信号的找全率达到了99%,错误率为0%;对实际采集的声信号进行Click信号检测,找全率为100%。本文方法预期为海豚观; 测和海豚生物学行为的研究提供一定的技术支持。A method based on Gabor filter is proposed for the detection of Pulses; of Bottlenose dolphin (Tursiops truncates). First, the acoustic signal; is divided into frames, and the spectrogram of each frame is calculated.; Then Gabor filter is designed to extract the vertical lines in the; spectrogram. Adaptive threshold is used to detect the regions where; pulses may be located in the spectrogram. Finally, the positions of the; pulses are determined by the connected component analysis. Simulating; test signal of different signal to noise ratio (SNR), the detection rate; is 99% and the error rate is 0% when SNR is 15 dB. Detecting pulses in; real world acoustic signal, the detection rate is 100%. The method is; expected to provide technical support for dolphin observation and the; study of dolphins' biological behavior

Additive manufacturing device and process for ceramic precursor material

本发明公开了一种用于陶瓷先驱体材料的增材制造装置和工艺，属于陶瓷材料的增材制造技术领域。该装置包括光路系统、支撑网板、升降机构、气氛循环及切换系统、成形腔和刮平机构；光路系统能够输出两种波长的激光光束，用于对工作区域进行扫描；采用光固化和原位烧结陶瓷化技术实现陶瓷类材料的直接成形，先平铺一层先驱体材料，采用光固化技术实现材料固化，再采用红外光烧结的方式将材料陶瓷化，如此依次逐层固化、逐层烧结最终实现复杂陶瓷结构的直接一体化成形。该成形工艺及装置可以实现先驱体材料陶瓷的一体化三维成形

一种基于增材缺陷的超声检测信号的降噪方法

本发明公开了一种基于增材缺陷的超声检测信号的降噪方法，属于增材制造技术领域。采用离散小波变换算法对增材缺陷的超声检测信号进行降噪处理，通过信号取样——离散小波分解——信号处理——离散小波重构获得降噪处理后的检测信号。在信号处理过程中采用了一种基于硬、软阈值法的可以动态调整的阈值函数。预制含有平底孔、气孔、及裂纹增材缺陷的激光增材样件用于检测并提取超声检测信号，对提出的降噪算法性能进行评估，通过对比不同降噪方法得到的降噪信号波形，以及这些信号的信噪比、均方根误差，发现提出的降噪算法优于传统的minimax软阈值法

10 Gb／s电吸收调制器的微波封装设计

在高速光电子器件的微波封装过程中，需要综合考虑封装寄生参数和芯片寄生参数对器件高频性能的影响。利用封装寄生参数对芯片寄生参数的补偿作用，成功实现了10Gb／s电吸收调制激光器（EML）的高频封装。通过封装前后芯片和器件的小信号频率响应测试结果对比，器件的反射参数和传输参数有所改善，3dB带宽达到10GHz；并进行了10Gb／s速率的光纤传输实验，经过40km光纤传输后通道代价不到1dBm（误码率为10^-12），满足10Gb／s长距离光纤传输系统的要求

基于常规测井方法估算原位水合物储集层力学参数

以南海神狐SH7井和阿拉斯加冻土区Mount Elbert井为例,基于常规油气储集层利用测井数据估算地层力学参数的方法,估算海洋区和冻土区水合物储集层力学参数,并与其他研究者的测试或计算结果进行对比。算例分析结果表明：根据常规油气储集层力学参数与声波波速间的关系式,利用测井结果可以估算海洋区水合物储集层的内聚力、内摩擦角、抗拉强度和抗剪强度,但海洋区水合物储集层泥质含量、冻土区水合物地层内摩擦角、海洋区和冻土区水合物地层杨氏模量及体积模量的估算结果误差较大;建立适用于水合物储集层的力学参数与声波波速或水合物饱和度间的关系式,并通过测井方法获取声波波速或水合物饱和度数据,可以更准确地估算原位水合物储集层力学参数

天然气水合物储层测井评价及其影响因素

天然气水合物储层准确识别和精细定量评价是自然界水合物开发利用和环境影响研究的基础，而地球物理测井则是除地震和钻探取心外最有效的原位识别和评价方法。电阻率和声波测井是最早应用于水合物储层识别的井内地球物理方法，由于具有较高的准确性和可靠性，二者仍是目前水合物测井的主要方式，并不断衍生出新的技术，如环电阻率、方向电阻率、多极声波测并等；同时，其他如井内成像、密度、电磁波、核磁共振等测井方法也被综合用于识别水合物储层。经过最近20年的发展，水合物系统测井方法已从单一的测井识别发展到运用各种先进的随钻和电缆测井评价复杂地质条件下水合物储层物性的阶段，初步建立了一套基于常规油气系统的水合物系统测井评价理论体系。该套体系对均质孔隙填充型砂质水合物储层具有较好的适用性，但对于非均质性泥质储层（如裂隙、薄层、互层、富泥质）及胶结或骨架支撑形式的水合物储层应用会产生较大的误差。此外，钻井过程中井眼冲蚀和钻井液侵入等外部因素也会影响水合物测井识别和评价结果。通过了解不同测井方法及其最新的研究进展，认为未来水合物测井技术的发展应侧重提高水合物地层识别和评价的准确性，开发复杂条件下水合物储层岩石物理模型，并综合利用地震、测井、岩心分析等方法修正所建立的岩石物理模型和校正实际的测井结果