The spatial and temporal distribution of virioplankton and heterotrophic bacteria in the coastal waters of Zhejiang

为了解浙江近海海域浮游病毒和异养细菌的时空生态分布,于2014年11月(秋)、2015年1月(冬)、2015年5月(春)和2015年7月(夏)连; 续4个季节采集了浙江近海海域表层海水样品,采用流式细胞仪技术对样品浮游病毒和异养细菌丰度进行了检测,对其时空分布特征及与环境因子的相关性做了分析; 。从水平分布来看,在4个季节中浮游病毒、异养细菌丰度均为宁波、沈家门、岱山等沿岸海域站位的丰度低,远陆海域东极和枸杞站位的丰度高。从季节变化来看; ,浮游病毒、异养细菌丰度的季节分布特征同为夏>春>秋>冬,相关性分析结果表明,春、夏、秋、冬4个季节,浮游病毒丰度与异养细菌丰度均为显著正相关。; 浮游病毒丰度在春、秋、冬季节均与病毒/细菌比值(VBR)显著正相关;夏、秋季节均与盐度显著正相关;春、夏季节均与总磷显著负相关;春季分别于与溶解; 氧、pH、化学耗氧量(COD)显著正相关。异养细菌在春、秋、冬季节均与VBR显著正相关;春、夏季节与溶解氧显著正相关,冬季与溶解氧显著负相关;春; 、夏季节与总磷显著负相关;秋、冬季节均与温度、盐度显著正相关;春、冬季节均与COD显著正相关。In order to investigate the spatial and temporal distribution of; virioplankton and heterotrophic bacteria in the coastal waters of; Zhejiang,the representative samples of sea water were collected in the; coastal waters of Zhejiang for 4 consecutive seasons in November; 2014(Autumn),January 2015(winter),May 2015(spring) and July; 2015(summer). The distribution of virioplankton and heterotrophic; bacteria was studied by flow cytometry,and the correlations between the; abundances of virioplankton,heterotrophic bacteria and environmental; factors were also analyzed. From the horizontal distribution,in the four; seasons low abundance of virioplankton and heterotrophic bacteria was in; the stations of Ningbo,Shenjiamen,Daishan immediate offshore; area;high-abundance was in the stations of Dongji and Gouqi offshore; area. From the seasonal distribution,the abundance of the virioplankton; and heterotrophic bacteria was the same as that of; summer>spring>autumn>winter. The abundance of virioplankton and; heterotrophic bacteria was significantly and positively correlated in; the 4 seasons. The abundance of virioplankton was significantly and; positively correlated with VBR in spring,autumn and winter. The; abundance of virioplankton was significantly and positively correlated; with salinity in summer and autumn. The abundance of virioplankton was; significantly and negatively correlated with TP in spring and summer.; The abundance of virioplankton was significantly and positively; correlated with dissolved oxygen,pH,COD in spring. The heterotrophic; bacteria were significantly and positively correlated with VBR in; spring,autumn and winter. The heterotrophic bacteria were significantly; and positively correlated with dissolved oxygen in spring and summer but; were significantly and negatively correlated with dissolved oxygen in; winter. The heterotrophic bacteria were significantly and negatively; correlated with TP in spring and summer. The heterotrophic bacteria were; significantly and positively correlated with temperature and salinity in; autumn and winter. The heterotrophic bacteria were significantly and; positively correlated with COD in spring and winter.国家海洋局公益性行业科研专

Spatial and Temporal Distribution of Picophytoplankton in Coastal Waters of Zhejiang

【目的】为了解浙江近海海域超微型光合自养浮游生物原绿球藻(Prochlorococcus,; Pro)、聚球藻(Synechococcus,Syn)和超微型真核浮游植物(Picoeukaryotes,Euk)的时空生态分布,【方法】于20; 14年11月(秋)、2015年1月(冬)、2015年5月(春)和2015年7月(夏)连续4个季节采集了浙江近海海域表层海水样品,采用流式细胞仪技; 术对样品超微型光合自养浮游生物丰度进行了检测,并对其时空分布特征及与环境因子的相关性进行了研究。【结果】从水平分布来看,在4个季节中超微型光合自; 养浮游生物丰度均为宁波、沈家门、岱山等沿岸海域站位的丰度低,远陆海域东极和枸杞站位的丰度高。从季节分布来看,原绿球藻的季节分布特征为夏季>秋季; >冬季>春季,聚球藻的季节分布特征为秋季 >冬季 >春季>夏季,超微型真核浮游植物的季节分布特征为夏季>秋季; >春季>冬季。相关性分析表明,春季,原绿球藻、聚球藻、超微型真核浮游植物均与pH显著正相关;秋季,原绿球藻、聚球藻、超微型真核浮游植物均与温度显; 著正相关;冬季,聚球藻、超微型真核浮游植物均与盐度显著正相关。【结论】本文超微型光合自养浮游生物丰度近岸海域明显低于远陆海域,这与近岸海域泥沙含; 量大,水色和透明度低有很大关系。浮游病毒对宿主的裂解也会影响超微型光合自养浮游生物的丰度变化。【Objective】 The preseat paper aims to investigate the spatial and; temporal distribution of picophytoplankton: Prochlorococcus,; Syn-echococcus and Picoeukaryotes in the coastal waters of; Zhejiang.【Method】 The representative samples of sea water in the coastal; waters of Zhejiang were collected for 4 consecutive seasons in November; 2014 (Autumn), January 2015 (winter),May 2015 (spring) and July 2015; (summer). The distributions of picophytoplankton by flow cytometry were; analyzed, and the correlation between the abundances of; picophytoplankton and environmental factors were studied.【Result】 From; the horizontal distribution, in the four seasons, the abundance of; picophytoplankton were low-abundance areas in the stations of; Ningbo,Shenjiamen, Daishan immediate offshore area, high-abundance areas; in the stations of Dongji and Gouqi offshore area. From seasonal; distribution, the abundance of Prochlorococcus was in order of summer >; autumn > winter > spring, the abundance of Synechococcus autumn > winter; > spring > summer; and the abundance of Picoeukaryotes summer > autumn >; spring > winter. Prochlorococcus,Synechococcus and Picoeukaryotes were; significantly positively correlated with pH in the spring.; Prochlorococcus,Synechococcus and Picoeukaryotes were significantly; positively correlated with temperature in autumn. Synechococcus and; Picoeukaryotes were positively correlated with salinity in; winter.【Conclusion】In this study, the abundance of picophytoplankton in; immediate offshore area was significantly lower than that of far; offshore area. The reason was that the sediment content was large,the; water color and transparency was low in immediate offshore area. The; cracking effect of the virioplankton on the host would affect the; abundance of picophytoplankton.国家海洋局公益性行业科研专