针刺大都穴治疗中风偏瘫疗效观察

目的:观察针刺大都穴对中风偏瘫下肢的影响及临床疗效。方法:将72例患者随机分成两组:治疗组37例采用基础穴位配合大都穴治疗;对照组35例采用基础穴位结合神经肌肉电刺激仪治疗,治疗1个月后比较两组的临床疗效。结果:通过下肢Fugl-Meyer量表评分比较,治疗组均优于对照组(P<0.05)。结论:基础穴位配合大都穴治疗中风偏瘫在改善下肢运动功能方面疗效较好

福建省中医风湿病学科发展报告

该文介绍了福建省中医风湿病学科发展历程、近代研究、主要相关成果及当前面临的挑战和机遇,重点分析了阻碍福建省中医风湿病学科发展的主要瓶颈问题,并提出今后发展的建议与展望

生物陶瓷隔药灸治疗宫颈癌术后尿潴留疗效观察

目的:观察生物陶瓷隔药灸治疗宫颈癌术后尿潴留的临床疗效。方法:将79例患者随机分成治疗组和对照组。治疗组选择生物陶瓷隔药灸疗法,将自拟活血温经方的药物研粉后外敷于关元、中极、双侧水道4个穴,以敷贴粘牢固定,再用加热过的生物陶瓷温灸球置于敷贴上温灸。对照组选择神经肌肉电刺激治疗。比较两组治疗后的残余尿量和临床疗效。结果:在改善残余尿量及临床疗效方面,治疗组均优于对照组。结论:生物陶瓷隔药灸治疗宫颈癌术后尿潴留有较好疗效

[Row acupuncture at the gaps between phalanges for dorsal stretch of fingers after cerebral infarction].

目的：比较指骨间缝排刺疗法与理疗治疗脑梗死后手指背伸功能障碍的临床疗效差异。方法：将71例患者随机分成指骨间缝排刺组（A组,37例）、理疗组（B组,34例）。两组在体针的基础上,A组选择指骨间缝排刺疗法,对患侧手指第Ⅰ、第Ⅱ、第Ⅲ骨间缝进行排刺,并予连续波电刺激;B组使用神经损伤治疗仪,将两电极放于前臂指伸肌肌腹中央处进行理疗。两组均每次治疗30min,每日1次,10d为一疗程,疗程间休息3d,共治疗3个疗程。比较两组患者治疗前后的掌指关节背伸活动度及指总伸肌肌力。结果：与治疗前比较,两组治疗后各指掌指关节背伸活动度及指总伸肌肌力均提高（均P〈0.05）;在改善示、中、环指掌指关节背伸活动度及指总伸肌肌力方面,A组优于B组（均P〈0.05）,但对小指掌指关节背伸活动度及指总伸肌肌力的改善两组间差异无统计学意义（均P〉0.05）。结论：在体针治疗基础上,指骨间缝排刺疗法治疗脑梗死后偏瘫手指背伸功能障碍有较好疗效,优于理疗。OBJECTIVE: To compare the effect differences between row acupuncture at the gaps between phalanges and physical therapy for the dorsal stretch of fingers after cerebral infarction. METHODS: Seven-one cases were randomized into a row acupuncture at the gaps between phalanges group (group A, 37 cases) and a physical therapy group (group B, 34 cases). Body acupuncture was applied in the two groups. Row acupuncture at the 1st, 2nd, and 3rd gaps between phalanges was used in the group A, with continuous wave of electroacupuncture. The two electrodes of nerve injury therapeutic apparatus were put in the muscle belly center of extensor digitorum of forearm in the group B. All the treatments were given for 30 min in the two groups, once a day and 10 times as one course. Three courses were required with 3 d at the interval. The dorsal stretch of metacarpophalangeal joints and the muscle force of common extensor of fingers were compared between the two groups before and after treatment. RESULTS: Compared with those before treatment, the dorsal stretch of metacarpophalangeal joints and the muscle force of common extensor of fingers were improved after treatment in the two groups (all P<0.05). There existed better effects in the group A on the dorsal stretch of the forefinger, middle finger and ring finger of metacarpophalangeal joints, as well as the muscle force of common extensor of fingers (all P<0.05). CONCLUSIONS: Row acupuncture at the gaps between phalanges achieves better effect than physical therapy for the dorsal stretch of fingers after cerebral infarction, based on body acupuncture

Prokaryotic Expression and Purification of Ca~(2+)-ATPase from Aquatic Plant Eichhornia crassipes

从水生植物凤眼莲叶片中提取总rnA,经rT-PCr扩增出CA2+-ATPASE基因片段,经限制性内切酶(SMA I,nOT I)酶切后按正确的读码框顺序插入到PgEX-4T-2表达载体上,重组质粒转化大肠杆菌,经菌落PCr和质粒双酶切鉴定、序列测定确认,证实成功地构建了CA2+-ATPASE基因融合表达载体,.转化菌经IPTg诱导表达,获得了大小约48kd的可溶性目的蛋白,与预期相吻合.利用谷胱甘肽琼脂糖凝胶4b(gluTATHIOnE SEPHArOSE 4b)亲和介质对重组蛋白进行纯化,获得了高纯度的目的蛋白.The Ca2+-ATPase gene was cloned fromEichhornia crassipes leaves using the PCR technology.After digested by the enzymes(Sma I,Not I),it was inserted into the plasmid pGEX-4T-2to reconstruct the expression vector.The recombinant protein was induced by IPTG and then purified using Glutathione Sepharose 4B.As a result,a single 48 kDa protein was acquired,which implied the protein was the purified Ca2+-ATPase fusion protein.国家自然科学基金面上项目(30770391

乳头瘤病毒及协同因子与宫颈癌的关系

本研究旨在探讨宫颈癌前病变和宫颈癌的发生发展与人乳头瘤病毒及协同因子（HSV，CMV）的关系。对81例不同宫颈病变组织进行HPV16／18和HPV6／11原位杂交，同时对103例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。结果表明病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16／18与不同宫颈病变组织原位杂交阳性率平均为51％，HPV6／11的则为64％。经PCR检测，HPV16／18、HPV6／11、HSV、CMV在不同宫颈病变组织中的阳性率分别为21％、4％、23％和0％。HSV可协同HPV16／18恶性转化宫颈上皮细胞，并对其协同机制进行了细胞及分子生物学的探讨

乳头瘤病毒及协同因子与宫颈癌的关系

本研究旨在探讨宫颈癌前病变和宫颈癌的发生发展与人乳头瘤病毒及协同因子（HSV，CMV）的关系。对81例不同宫颈病变组织进行HPV16／18和HPV6／11原位杂交，同时对103例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。结果表明病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16／18与不同宫颈病变组织原位杂交阳性率平均为51％，HPV6／11的则为64％。经PCR检测，HPV16／18、HPV6／11、HSV、CMV在不同宫颈病变组织中的阳性率分别为21％、4％、23％和0％。HSV可协同HPV16／18恶性转化宫颈上皮细胞，并对其协同机制进行了细胞及分子生物学的探讨

乳头瘤病毒及协同因子与宫颈癌的关系

本研究旨在探讨宫颈癌前病变和宫颈癌的发生发展与人乳头瘤病毒及协同因子 (HSV ,CMV)的关系。对81例不同宫颈病变组织进行HPV16 / 18和HPV6 / 11原位杂交 ,同时对 10 3例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。结果表明病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16 / 18与不同宫颈病变组织原位杂交阳性率平均为 5 1% ,HPV6 / 11的则为 6 4%。经PCR检测 ,HPV16 / 18、HPV6 / 11、HSV、CMV在不同