行政院國家科學委員會專題研究計畫果報告書:石蠟塊切片的基因型鑑定

在20 例研究案例中，藉由案例中血液的POLYMARKER DNA 基因型，與同一人石蠟塊 切片的POLYMARKER DNA 基因型的交叉校對後，所有20 例研究案例均能順利的由石蠟塊 切片中獲知其POLYMARKER DNA 基因型，其結果與血液的結果吻合。 以上實驗結果顯示，利用石蠟塊切片組織來進行DNA 基因鑑定是一種可行而有價值的 的方法。藉由本研究的方法，我們可以進一步地推動各種相關的研究，也可應用於法醫學的 鑑定和人身鑑別工作，實在是非常有值得利用的一種方法。From 1998 to 1999, 20 cases of resected liver tissue in the Department of Patholology of National Taiwan University Hospital were studied for their tissue genomic type in the paraffinized sections. All these 20 cases were successful to extract their tissue DNA and typing by the AmplitypeÒ PM PCR Amplification and Typing Kit. The AmplitypeÒ PM PCR Amplification and Typing Kit is a very powerful and useful tool to identify the individual genetic pattern in forensic fields. By using the application of AmplitypeÒ PM PCR Amplification and Typing Kit, we demonstrate a clinical, practical way to resolve the problems of genomic typing in the paraffinized tissue in the forensic pathology. Also, it is a quickly, easy, specific and scientific way to perform in the molecular laboratories within 3 days. In conclusion, it is possible to resolve the clinical and medicolegal problems of genomic typing in paraffin-embedded biopsies by using the AmplitypeÒ PM PCR Amplification and Typing Kit now

Clinicopathology of Severe Acute Respiratory Syndrome: An Autopsy Case Report

In mid-April 2003, a major outbreak of severe acute respiratory syndrome (SARS) developed in Taiwan. During the outbreak, SARS-associated coronavirus (SARS-CoV) was documented in 346 patients and 73 of them died. Autopsy was performed in 9 of the suspected SARS patients who died during the outbreak, but SARS was the cause of death in only 1 of these patients . Here we report the histological features of this patient and their clinicopathological correlations. The patient, a 36-year-old Indonesian woman, was a caretaker working for a Taiwanese family. She stayed in Taipei Jen-Chi Hospital from April 10 to April 19 to take care of her elderly employer. She developed fever on April 21 and respiratory distress on April 25, and received ribavirin, intravenous immunoglobulin, and steroid. The respiratory distress persisted and worsened, and intubation was performed on April 27. The respiratory condition improved initially after mechanical ventilation, but subcutaneous emphysema and pneumomediastinum developed on May 1. Her condition deteriorated rapidly and she died on May 2, 11 days after the onset of fever. Autopsy was performed on the same day. Histologically, the lungs showed severe diffuse alveolar damage and bronchopneumonia, but no viral inclusion. The spleen and lymph nodes revealed lymphoid depletion and the liver showed microvesicular steatosis. No specific pathological change was seen in the gastrointestinal tract and kidneys. SARS-CoV genome was detected in the nasopharyngeal aspirate and the autopsy lung specimen

Can leflunomide play a role in cytomegalovirus disease prophylaxis besides its antirheumatic effects?

Leflunomide, a disease-modifying antirheumatic drug (DMARD), has shown effectiveness in many autoimmune disorders and can suppress cytomegalovirus (CMV) disease in humans. Thus, it is possible for leflunomide to play a role in CMV prophylaxis or pre-emptive therapy in high-risk patients in addition to its application as a DMARD for autoimmune disorders. We describe two adult patients who developed CMV disease after administration of low-dose leflunomide. One patient was successfully treated with an increasing leflunomide dosage, resulting in a long-term suppression of serum CMV viral load

現代法醫病理學的發展與演變

在現代法醫病理學的領域中，最主要的演變和發展是： (1)分子生物學新科技的發展 對法醫病理學家的工作內容和觀念有很大的變格。 ( 2)特殊的高致死族群和新疾病 的不斷增加，造成非自然死亡的死亡案例劇增，因而形成對法醫病理學家們的一種挑 戰和衝擊，對法醫病理知識的要求也不斷地增加 。#c389300

法醫病理學的演變

在1980年代，隨著醫學及病理學的不斷進步，在法醫學病理學的領域中，有二個重要 的發展，一是藥物性猝死案例的研究及探討，二是dna 紋圖的發展與應用。二者均為 法醫學界帶來極大的衝擊及考驗，亦成為法醫病理學蛻變的契機。#c389300

1980年代解剖病理學的發展與演變

總括來說，1980年代解剖病理學的發展與演變可歸納成幾個方面來說明： (1)傳統病 理學觀念的衝擊和突破。 (2) 解剖率的持續下降。(3)病理學界所達成的成就。 (4) 新技術的應用和展望。 (5)臨床醫學和病理學的融合。#c389200