高效液相色谱-质谱联用法识别明胶动物来源

<正>明胶是动物胶原蛋白经化学处理得到的多肽混合物,具有良好的水溶性,其水溶液随温度改变可在溶胶与凝胶间发生可逆转变。明胶的这种独特物理性质使其在医药、食品、化妆品、感光材料及其它领域获得广泛应用。制备明胶的主要原料是牛骨、猪骨或猪皮等,动物来源、年龄以及胶原蛋白类型等对明胶的性质均有不同

高效液相色谱质谱联用法复合干扰素二硫键定位分析

<正> 重组复合干扰素(rIFN-con)是一种由166个氨基酸组成的蛋白质,含有四个半光氨酸分别是Cys1,Cys29,Cys99,Cys139。本研究利用高效液相色谱-电喷雾质谱对复性后的重组复合干扰素中的二硫键进行了定位分析,结果表明rIFN-con中的两对二硫键为Cys1-Cys99和Cys29-Cys139。复性得到的rIFN-con经质谱分析其分子量

液相色谱-电喷雾离子阱质谱法分析明胶琥珀酰化修饰位点

琥珀酰明胶是目前临床上广泛使用的血浆代用品之一,其临床疗效与明胶分子量分布及琥珀酰化程度密切相关。作为明胶修饰剂的琥珀酸酐主要与明胶分子中的自由氨基发生取代反应,本研究对明胶和琥珀酰化明胶进行了液质联用分析,确定了用于明胶代血浆制备的明胶原料的羟基化修饰位点,并进一步获取了明胶的琥珀酰化修饰行为和位点的信息

determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting

The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin

determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting

The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin

基于串联质谱的鱼皮明胶鉴别研究

在胶原蛋白序列比对基础上,以虹鳟鱼明胶、猪明胶和牛明胶为模型,利用高效液相色谱-串联质谱技术(HPLC-MS/MS)研究了3种明胶降解多肽组成的差异。使用胰蛋白酶将鱼皮明胶进行了酶解处理,使用HPLC-MS/MS对酶解产物中的多肽组成进行了分析,并与猪和牛明胶酶解产物中的多肽进行了比较。结果表明鱼明胶酶解产物中存在特征多肽,通过特征多肽的种类可区别鱼明胶与猪和牛明胶,研究了明胶多肽中脯氨酸羟基化修饰、明胶分子量范围和脱酰胺化对特征多肽识别的影响。研究表明利用HPLC-MS/MS技术通过识别明胶酶解产物中的特征多肽进行鱼皮明胶鉴别具有可行性

基于串联质谱的鱼皮明胶鉴别研究

在胶原蛋白序列比对基础上，以虹鳟鱼明胶、猪明胶和牛明胶为模型，利用高效液相色谱-串联质谱技术（HPLC—MS／MS）研究了3种明胶降解多肽组成的差异。使用胰蛋白酶将鱼皮明胶进行了酶解处理，使用HPLC—MS／MS对酶解产物中的多肽组成进行了分析，并与猪和牛明胶酶解产物中的多肽进行了比较。结果表明鱼明胶酶解产物中存在特征多肽，通过特征多肽的种类可区别鱼明胶与猪和牛明胶，研究了明胶多肽中脯氨酸羟基化修饰、明胶分子量范围和脱酰胺化对特征多肽识别的影响。研究表明利用HPLC—MS／MS技术通过识别明胶酶解产物中的特征多肽进行鱼皮明胶鉴别具有可行性

阿胶中特异性多肽的分离与液质联用分析

<正>阿胶(Colla corri asini)是我国的一种传统中药,是由驴皮经煎煮浓缩制成的固体胶,其主要成分为蛋白质和多肽类物质、多糖类物质及其它小分子物质。阿胶的主要功能包括补血、免疫功能调节、抗休克、促进淋巴细胞转化及改善钙代谢等,但作用机理并不十分明确。近年来,关于胶原蛋白及明胶中的活性多肽的相关研究逐步成为研究热点。阿胶中蛋白质和多肽类物