Sexual dimorphism in relationships between of plasma urea and some psycho-neuro-endocrine parameters

Background. Earlier we found a strong urato-neural relationships in both men and women. Moreover, in women, the factor structure of the neural root is almost completely different from that in men. In addition, significant differences were found between the profiles of urato-neural correlations of women of reproductive age and postmenopausal women. The purpose of this study is to analyze the relationships between the plasma urea level and some psycho-neuro-endocrine parameters in the same cohort. Materials and Methods. The object of observation were almost healthy volunteers: 31 males (24÷69 y) and 30 females, from among them 18 postmenopausal (48÷76 y) and 12 of reproductive age (30÷45 y). In basal conditions we determined plasma levels of urea and adaptation hormones, estimated the severity of the trait and reactive anxiety, recorded the ongoing HRV and EEG. After 4 or 7 days, repeated testing was performed. Results. By building regression models with stepwise exclusion, it was found that in men plasma urea positively determines (R2=0,499) the level of circulating catecholamines and power spectrum density (PSD) of δ-, θ- and β-rhythms in different loci. In postmenopausal women, urea downregulates the amplitude and PSD of δ-rhythm in different loci, instead, it upregulates the PSD of α-rhythm in T5 locus, entropy of EEG in 2 loci and vagal tone. The degree of determination of neural parameters is 71,6%. In women of reproductive age the degree of determination of neural parameters is similar (69,9%), but the factor structure of the model is completely different. Urea upregulates the PSD of β-rhythm in F7 locus while downregulates the levels of reactive anxiety, vagal tone, entropy of EEG in 3 loci and P4-θ PSD as well as causes left lateralization of θ- and α-rhythms. Conclusion. Plasma urea has a modulating effect on EEG&HRV parameters, as well as reactive anxiety, but this effect is significantly different in men and women of different ages

Neuroendocrine-immune relationships at rats regardless of sex and exposure to stressors or adaptogens

Introduction and aim. Since the discovery of the famous Selye triad, studies of the connections between adaptation hormones and the immune system have remained relevant. We formulated the aim of the future study: in the mode of synchronicity, to reveal sexual differences in the parameters of the neuroendocrine-immune complex and the state of neuroendocrine-immune relationships in intact rats and exposed to stressors and adaptogens. This article initiates the beginning of the movement towards achieving the goal. Material and methods. The experiment is at 96 rats Wistar line: 48 males and 48 females. Over the 12 days, one male and female rat remained intact and 3 other pairs were exposed to chronic aversive stress for 6 days. We calculated the parameters of the HRV: Mode, Amplitude of the mode and Variational scope as markers of the circulating catecholamines, sympathetic and vagal tones respectively. Among endocrine parameters determined excretion of 17-Ketosteroides and serum levels of main adaptation hormones such as Corticosterone, Aldosterone, Testosterone, Triiodothyronine, as well as Parathyroid hormone and Calcitonin. The percentage of lymphocyte populations and the parameters of phagocytosis by neutrophils and monocytes of Staphylococcus aureus were determined in the blood. The Thymus and Spleen were weighed and made smears-imprints for counting Thymocytogram and Splenocytogram. Results. The canonical correlation between neuroendocrine and immune parameters was analyzed. The most pronounced immunomodulatory effect is exerted by catecholamines (R=0.928), sympathetic tone (R=0.849), and testosterone (R=0.829). Vagal tone (R=0.697), 17-ketosteroides (R=0.688), and aldosterone (R=0.686) moderately modulate immunity, while the immunomodulatory effects of triiodothyronine (R=0.610), corticosterone (R=0.584), PTH (R=0.510), and calcitonin (R=0.423) are the least pronounced. Three pairs of canonical roots were found. The neuroendocrine root of the first pair determines the parameters of immunity by 95.7%, the second pair by 86.5%, and the third pair by 69.2%. The elements of the Thymocytogram (in descending order: epitheliocytes, lymphocytes, endotheliocytes, lymphoblasts, and macrophages), the phagocytic activity of blood neutrophils and monocytes, the relative content of NK- and B-lymphocytes in the blood, as well as the percentage of lymphocytes and macrophages in the Splenocytogram turned out to be most influenced by neuroendocrine factors. Conclusion. There is a close canonical correlation between registered neuroendocrine factors and immunity parameters in general. At the same time, both the severity of the immunomodulatory activity of individual neuroendocrine factors and the subjection to the regulatory influence of individual parameters of immunity differ significantly

Sexual dimorphism in relationships between of plasma uric acid and some psycho-neuro-endocrine parameters

Background. Earlier we found that effects of plasma nitrogenous metabolites as urea, creatinine, and bilirubin on neuro-endocrine parameters, as well as anxiety, are significantly different in men and women of different ages. The purpose of this study is to analyze the relationships between the plasma uric acid level and some psycho-neuro-endocrine parameters in the same cohort. Materials and Methods. The object of observation were almost healthy volunteers: 31 males (24÷69 y) and 30 females, from among them 18 postmenopausal (48÷76 y) and 12 of reproductive age (30÷45 y). In basal conditions we determined plasma levels of uric acid and adaptation hormones, estimated the severity of the trait and reactive anxiety, recorded the ongoing HRV and EEG. After 4 or 7 days, repeated testing was performed. Results. By uric acid regression models with stepwise exclusion, it was found that in men plasma uric acid downregulates the PSD of β-rhythm in P4 and δ-rhythm in others 4 loci, but upregulates the Asymmetry of δ-rhythm, variability of α-rhythm, PSD of θ-rhythm in P3 and O1 loci, Entropy of EEG in T4 as well as Testosterone plasma level. The measure of determination is 54,5%. In postmenopausal women, uricemia downregulates the Amplitude of β-rhythm and its PSD in 8 loci as well as sympathetic tone, but upregulates the PSD of HF and VLF bands HRV, Testosterone plasma level, PSD of θ-rhythm in F3 and O2 loci as well as the Laterality of δ-rhythm. The degree of determination of neuro-endocrine parameters is 94,4%. In women of reproductive age uricemia upregulates the Asymmetry and Amplitude of β-rhythm and its PSD in 6 loci, the Amplitude and Laterality of θ-rhythm and its PSD in F8 locus, as well as the PSD of α-rhythm in F8 locus. The degree of positive determination of EEGs parameters is 95,5%. Conclusion. Plasma uric acid has a modulating effect on neuro-endocrine parameters, but this effect is significantly different in men and women of different ages, which is due, apparently, to the influence of sex hormones on the expression of adenosine receptors in neurons

Immediate effects of Ukrainian phytocomposition on biophotonics (GDV), EEG and HRV parameters

Background. Back in 2015, we registered short-term reactions to the Ukrainian phytocomposition of electroencephalogram and heart rate variability parameters. At the same time, we also recorded the reactions of biophotonics (GDV) parameters, but due to the skepticism of Ukrainian academic science regarding this method, we did not dare to include the results in the article. Materials and methods. The object of observation were 12 women (44±13 years) and 62 men (44±12 years) with dysfunction of neuro-endocrine-immune complex. GDV, HRV and EEG parameters were recorded in the morning in basal conditions. Then the members of the main group used 5 ml of phytocomposition dissolved in 45 ml of tap water, instead in the control group used 50 ml of the latter. After 1,5 hours, the test was repeated. Results. Discriminant analysis revealed 26 EEG, 6 HRV and 7 GDV parameters characteristic of the initial state and after consumption of phytocomposition or tap water. The use of balm causes the normalizing decrease of increased sympathotonic markers and the increase of decreased vagotonic markers. Physiologically favorable vegetotropic effects of the balm are accompanied by a further increase in the initially increased activity of β-rhythm-generating cortical and subcortical structures as well as activation of θ-rhythm-generating and inhibition of α- and δ-rhythm-generating nuclei whose initial activity was within normal limits. Neurotropic effects are accompanied by a decrease in fractality and entropy and an increase in the area of GDI, as well as the energy of the first, fourth, fifth and seventh chakras (virtual). Conclusion. Ukrainian phytocomposition “Balm Truskavets’” causes favorable immediate neurotropic and biophysic changes at patients with dysfunction of neuro-endocrine-immune complex

Uric acid, neuroendocrine-immune complex and metabolism: relationships

Background. During the implementation of the project "Physiological activity of uric acid", our group discovered four variants of the combination of levels of uricemia and uricosuria in patients with chronic pyelonephritis in the remission phase, which are accompanied by characteristic constellations of parameters of the central and autonomic nervous, endocrine, and immune systems, as well as the exchange of nitrogenous metabolites and electrolytes, the levels of which correlate with uricemia and/or uricosuria. The aim of this study is to clarify the relationship between parameters of uric acid exchange and neuroendocrine-immune complex as well as other metabolites. Materials and methods. Under an observations were 34 males (23-70 years) and 10 females (33-76 years) with chronic pyelonephritis in the phase of remission. The main object of the study was serum and urine levels of uric acid. Other metabolic, endocrine and immune parameters were determined in the same blood and urine samples. In addition, EEG and HRV was recorded almost synchronously. Results. Among all registered parameters, 28 were identified as characteristic of the four variants of uric acid metabolism. The discriminant model includes, in addition to uricosuria and uricemia by definition, 10 neuroendocrine, 5 immune, and 6 metabolic parameters, as well as bacteriuria, Bifidobacteria of feces, entropy of immunocytogram and Popovych’s leukocytogram strain index. According to the results of the canonical correlation analysis, it was established that balneotherapy-induced concomitant changes in uricosuria and uricemia positively determine changes in, first, the PSD of the theta-rhythm in the T3 locus and beta-rhythm in the O2 locus aa well as HRV-markers of vagal tone and sympatho-vagal balance; secondly – diuresis and excretion of urea, magnesium, sodium, phosphates, calcium, potassium and chloride, as well as calciumemia and magnesiumemia; thirdly – serum IgG and CIC. Instead, changes in cortisolemia and testosteroneemia as well as TNF-alpha and the intensity of Staph. aureus phagocytosis are subject to negative determination. In general, the rate of uric acid determination of the dynamics of the listed parameters of the body is 96%. Conclusion. The uric acid molecule, as a structural analog of methylxanthines and adenosine, exerts effects on neurons, endocrinocytes, and immunocytes, presumably through their adenosine receptors, and the metabolic effects of uric acid are the consequences of its neuro-endocrine effects

Vegetative, metabolic and immune accompaniments of changes in the electrokinetic index of the buccal epithelium under the influence of therapeutic factors

Background. In previous studies, we have shown that electrokinetic index of buccal epithelium (EKI) correlated with some functional and metabolic parameters. Subsequent studies have shown that сhanges in EKI correlated with changes in some parameters of EEG, HRV, hemodynamics, metabolism, immunity and fecal microbiocenosis. Further research in this direction was continued on a significantly increased contingent of patients and with the involvement of new methods and factors of influence. This message starts the presentation of the obtained results. Material and methods. Under a observations were 44 men (49±15 years) and 30 women (51±13 years) without clinical diagnosis or with chronic pyelonephritis in the phase of remission (23 men). We registered caused by the various therapeutic factors changes in EKI, state of the vegetative and hormonal regulation as well as immunity and metabolism, then calculated relationships between changes. Results. In 49 patients the changes in EKI were in the range of ±2,5%, in 19 people EKI increased by more than 2,5% (M±SD=+4,0±1,6%), while in 9 people decreased by more than 2,5% (-4,2±1,7%). The canonical correlation between changes in EKI, on the one hand, and HRV and immunity parameters, on the other, is moderate: R=0,478; p=0,023. The method of discriminant analysis revealed 10 immune and 6 HRV parameters as well as triglycerides and cholesterol, whose changes are characteristic of multidirectional changes in EKI. Conclusion. Electrokinetic index of buccal epithelium responds to therapeutic factors in different directions, accompanied by characteristic changes in a number of parameters of HRV, immunity and metabolism

Sexual dimorphism in relationships between of plasma creatinine and some neuro-endocrine parameters

Background. Earlier we found that plasma uric acid and urea causes modulating effects on neuro-endocrine parameters, as well as reactive anxiety, but these effects are significantly different in men and women of different ages. The purpose of this study is to analyze the relationships between the plasma creatinine level and some psycho-neuro-endocrine parameters in the same cohort. Materials and Methods. The object of observation were almost healthy volunteers: 31 males (24÷69 y) and 30 females, from among them 18 postmenopausal (48÷76 y) and 12 of reproductive age (30÷45 y). In basal conditions we determined plasma levels of creatinine and adaptation hormones, estimated the severity of the trait and reactive anxiety, recorded the ongoing HRV and EEG. After 4 or 7 days, repeated testing was performed. Results. By building regression models with stepwise exclusion, it was found that in men plasma creatinine downregulates the level of Cortisol (r=-0,41) while upregulates the levels of Testosterone (r=0,36) and Calcitonin (r=0,30). The degree of determination of endocrine parameters is 31,9%. In postmenopausal women, creatinine also upregulates Testosterone (r=0,43) while downregulates the amplitude and PSD of β-rhythm in different loci as well as causes right lateralization of β-rhythm. The degree of determination of neuro-endocrine parameters is 61,2%. In women of reproductive age creatinine upregulates the PSD of ULF band HRV and α-rhythm in 5 loci as well as the Entropy of EEG in F3 locus while downregulates the Entropy in T6 locus, F3-δ and T6-θ PSD as well as Cortisol level. The degree of determination of neuro-endocrine parameters is 94,0%. Conclusion. Plasma creatinine has a modulating effect on neuro-endocrine parameters, but this effect is significantly different in men and women of different ages

“Myroslava” and “Khrystyna” drinking mineral waters modulate the state of neuroendocrine-immune complex and metabolism in patients of Truskavets’ spa

Background. Earlier in an experiment on rats we showed that newly created sulfate-chloride sodium-magnesium drinking mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa has a significant modulating effects on the parameters of metabolism and the autonomic nervous, endocrine and immune systems. Adhering to the principle "Ex experimento ad clinic", we continued research in this direction with the participation of patients of the resort. We showed that these mineral waters have favorable effects on metabolic, HRVs, EEGs, endocrine and immune parameters. This article presents an integrated assessment of previously identified effects. Materials and Methods. The object of clinical-physiological observation were 34 men aged 23-70 years, who underwent rehabilitation treatment of chronic cholecystitis and pyelonephritis in remission in the Truskavets’ spa. The examination was performed twice, before and after a 7-10-day course of balneotherapy. All patients received bioactive water Naftussya, however, 11 men additionally drank water "Khrystyna", and the other 11 men water "Myroslava". The subject of the study were the parameters of the neuroendocrine-immune complex and metabolism. Results. The complex balneotherapy by interval use of sulfate-chloride sodium-magnesium mineral waters with Naftusya water causes significant changes in the constellation of EEGs, HRVs, endocrine, metabolic and immune parameters, which are different from the effects of Naftusya water monotherapy. Own effects of mineral waters are estimated by modeling. In general, the effects are physiologically favorable and have a normalizing nature. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have favorable neuroendocrine, metabolic and immune effects on patients with chronic cholecystitis and pyelonephritis

Peculiarities of relationships between plasma levels of nitrogenous metabolites and EEG & HRV parameters in patients with postradiation encephalopaty

Background. Recently we shown that nitrogenous plasma metabolites (uric acid, bilirubin, urea and creatinine), even in the absence of uremia, are able to influence the state of the anxiety, autonomic and central nervous and endocrine systems, apparently through aryl hydrocarbon and adenosine receptors of neurons and endocrine cells and/or directly. Sexual dimorphism in the neurotropic effects of uric acid in neurologically healthy patients was also revealed. The purpose of this study is to compare nitrogenous-neural relationships in neurologically healthy men and those with post-radiation encephalopathy (PREP). Materials and Methods. The object of observation were neurologically healthy 31 men (24÷69 y) and 19 patients (26÷61 y) with PREP. The relationships between plasma levels of nitrogenous metabolites, on the one hand, and EEG and HRV parameters, on the other, were analyzed. Results. By constructing regression models with stepwise elimination it was found that the multiple correlation coefficient (R±µ) of creatinine with neural parameters in patients with PREP significantly exceeded that of control patients (0,762±0,069 vs 0,409±0,107; t=2,62; p=0,011). With regard to urea, the differences are insignificant (0,801±0,059 vs 0,694±0,066; t=1,17; p>0,2), and with regard to bilirubin (0,548±0,115 vs 0,402±0,107; t=0,94) and uric acid (0,496±0,124 vs 0,549±0,089; t=0,32), there are practically no differences. Conclusion. Post-radiation encephalopathy is accompanied not only by deviations from the norm of a number of EEG and HRV parameters, but also by their increased sensitivity to creatinine and, to a lesser extent, urea, but not to bilirubin and uric acid

Relationships between changes in EEG and some metabolic parameters in patients of Truskavets’ spa

Background. Earlier, we found relationships between EEG/HRV parameters and plasma/ urine electrolytes concentration in Truskavets’ spa patients. The aim of this study is to analyze the relationships between changes in these parameters under the influence of balneotherapy. Materials and Methods. The object of observation were 22 men (23-70 years), who underwent rehabilitation treatment of chronic pyelonephritis and cholecystitis in remission. The examination was performed twice, before and after a 9-11-day course of balneotherapy. The object of the study were the parameters of the EEG and exchange of electrolytes and nitric metabolites. Results. Judging by the coefficient of determination, changes in the concentrations of electrolytes and nitric metabolites in the urine and plasma are mediated by changes in neurodynamics by 99,8 % and 99,4% respectively. Conclusion. Balneotherapy affects the exchange of electrolytes and nitrogen metabolites through the CNS. &nbsp