Screening of chemical composition of summer savory essential oils using solid-phase microextraction and GC-MC

Problems related to medicinal, spice (aromatic) plants species diversity, enhancing and improving human health are solved according to the Global Strategy for Plant Conservation and the Convention on Biological Diversity adopted regulations [1, 2]. The aim of this study is to investigate chemical composition of medicinal (aromatic) plant Summer savory. The object – Summer savory (Satureja hortensis L.) – annual herbaceous plant from Lamiaceae Lindl. introduced in Lithuania in Kaunas Botanical Garden of Vytautas Magnus University since 1962 [3, 4]. The raw material of the medicinal plant is herb of Savory (Herba Saturejae). Aerial parts of plant are widely used in traditional medicine for cramps, digestion disorders and inflammations. Savory has antimicrobial, antiviral, antispasmodic, anti-inflammatory, antifungal and antidiarrheal properties related to high concentration of essential oils and polyphenols. The main biological active substances of essential oils are carvacrol, thymol, γ-terpinen, thymoquinone, o-cymene and β-caryophyllene. Phytochemical analysis of medicinal plant was performed using solid-phase microextraction and gas chromatography-mass spectrometry by determining essential oil components of Satureja hortensis L. raw material [5, 6]. The results obtained during qualitative and quantitative evaluation of the raw material essential oil demonstrated that this medicinal (aromatic) plant can be successfully cultivated in Lithuania and used in the pharmaceutical and food industriesBiochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Pharmacognostical properties of medicinal plant Satureja hortensis L. introduced in Lithuania

Biochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Determination of total phenolics, flavonoids and radical scavenging activity of Summer savory (Satureja hortensis L.) methanolic extracts

The aim was to estimate changes of total phenolics, total flavonoids and radical scavenging activity of Summer savory (Satureja hortensis L.) methanolic extracts during vegetation in middle Lithuania edafo-climatic conditions. The object. Summer savory (Satureja hortensis L.) is the annual herbaceous plant from Labiatae Lindl. family originated from Mediterranean area and Middle East. This medicinal (aromatic) plant has been introduced in Kaunas Botanical Garden of Vytautas Magnus University since 1962 [1]. According to the literature summer savory has significant antioxidant activity related to the high phenolic content [2,3,4]. The raw material – Savory herb (Herba Saturejae) is widely used in traditional medicine for treatment of digestion disorders, cramps, cold and respiratory infections [5]. In this study phytochemical analysis of medicinal plants methanolic extracts was performed using spectrophotometry methods. The Folin-Ciocalteu spectrophotometric method was applied for determination of the total phenolic content. Estimation of total flavonoids was determined using a colorimetric aluminum chloride method. In order to detect antioxidant activity, a method based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was carried out. Results. The amounts of total phenolics, total flavonoids and radical scavenging activity were determined in five vegetation phases (intensive growing, butonisation, beginning of flowering, massive flowering, end of flowering). Spectrophotometric analysis revealed, that total amount of phenolic compounds and flavonoids in Satureja hortensis L. significantly changed during the plant vegetation. The highest concentration of these compounds was determined in butonization phase. The radical scavenging activity was caried out using DPPH radical, the correlation between total phenolic content with radical scavenging activity was observed. Conclusion. Determined phenolic comp...[...]Biochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Evaluation of chemical composition, radical scavenging and antitumor activities of Satureja hortensis L. herb extracts

Article Number: 53Satureja hortensis L. is an annual herbaceous plant of the Lamiaceae Lindl. family. S. hortensis L., related to thyme and rosemary, is used as spice and traditional medicinal herb in Europe. Mainly due to the polyphenols contained in S. hortensis L., this plant exhibits multiple biological effects. However, therapeutic effects on cells, including skin tumors, have not yet been studied. Therefore, the aim of this study was to compare the composition and the resulting antioxidant as well as biological properties [on melanocytes and melanoma cells] of summer, savory growing in botanical garden of Vytautas Magnus University in middle Lithuania climatic conditions, collected during various phases of vegetation. It has been shown that the budding phase alcohol extract of this plant contains the largest amounts of polyphenols, including rutin and rosemary acid, which promote the radical scavenging activity and antioxidant properties. In contrast, the extract from the massive flowering phase already at a concentration of 12.5 µg/mL reduces the survival of melanoma cells to 60% with 90% melanocytes survival. In addition, extracts from beginning of flowering and end of flowering at a concentration of 25 µg/mL, containing significantly less rutin and rosmarinic acid, in combination with irradiation of cells with UVB, significantly increased the lipid peroxidation process, particularly in melanoma cells. These data indicate the possibility of using extracts from S. hortensis L. to modulate/differentiate the metabolism of normal and tumor skin cellsInstrumentinės analizės APCLietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta