Toward eradication of B-vitamin deficiencies : considerations for crop biofortification

'Hidden hunger' involves insufficient intake of micronutrients and is estimated to affect over two billion people on a global scale. Malnutrition of vitamins and minerals is known to cause an alarming number of casualties, even in the developed world. Many staple crops, although serving as the main dietary component for large population groups, deliver inadequate amounts of micronutrients. Biofortification, the augmentation of natural micronutrient levels in crop products through breeding or genetic engineering, is a pivotal tool in the fight against micronutrient malnutrition (MNM). Although these approaches have shown to be successful in several species, a more extensive knowledge of plant metabolism and function of these micronutrients is required to refine and improve biofortification strategies. This review focuses on the relevant B-vitamins (B1, B6, and B9). First, the role of these vitamins in plant physiology is elaborated, as well their biosynthesis. Second, the rationale behind vitamin biofortification is illustrated in view of pathophysiology and epidemiology of the deficiency. Furthermore, advances in biofortification, via metabolic engineering or breeding, are presented. Finally, considerations on B-vitamin multi-biofortified crops are raised, comprising the possible interplay of these vitamins in planta

Cash transfers in an epidemic context : the interaction of formal and informal support in rural Malawi

This paper investigates the short-run consumption expenditure dynamics and the interaction of public and private arrangements of ultra-poor and labor-constrained households in Malawi using an original dataset from the Mchinjii social cash transfer pilot project (one of the first experiments of social protection policies based on unconditional cash transfers in Sub-Saharan Africa). The authors exploit the unique source of exogenous variation provided by the randomized component of the program in order to isolate the effect of cash transfers on consumption expenditures as well as the net crowding out effect of cash transfers on private arrangements. They find a statistically significant reduction effect on the level of consumption expenditures for those households receiving cash transfers, thus leading to the rejection of the perfect risk sharing hypothesis. Moreover, by looking at the effects of cash transfers on private arrangements in a context characterized by imperfect enforceability of contracts and by a social fabric heavily compromised by high HIV/AIDS rates, the analysis confirms the presence of crowding out effects on private arrangements when looking at gifts and (to a lesser extent) remittances, while informal loans seem to be completely independent from the cash transfer's reception. From a policy perspective, the paper offers a contribution to the evaluation of the very recent wave of social protection policies based on (unconditional) cash transfers in Sub-Saharan Africa, suggesting that there might be an important role for public interventions aimed at helping households to pool risk more effectively.Safety Nets and Transfers,Rural Poverty Reduction,Labor Policies,Services&Transfers to Poor,Debt Markets

Analysing how constraints impact architectural decision-making

Architectural design projects are characterised by a high number of constraints. Along with planning, energy performance and fire safety regulations, current designers have to face constraining factors related to budget, acoustics, orientation, wind turbulence, accessibility for the disabled, and so forth. These constraints steer the design process implicitly and explicitly in certain directions as soon as architectural designers aim at satisfying design briefs. We aim in this article at analysing the impact of such constraints on the design process. At this end, we have studied four design sessions in a particular (student) design use case. In analysing these four sessions, we used linkography as a method, because this appeared to be one of the better options to obtain a more quantitative assessment of the design process. The linkography method was combined with an interview of the student design team, in order to check the correctness of our conclusions

Quantitive analysis of electric vehicle flexibility : a data-driven approach

The electric vehicle (EV) flexibility, indicates to what extent the charging load can be coordinated (i.e., to flatten the load curve or to utilize renewable energy resources). However, such flexibility is neither well analyzed nor effectively quantified in literature. In this paper we fill this gap and offer an extensive analysis of the flexibility characteristics of 390k EV charging sessions and propose measures to quantize their flexibility exploitation. Our contributions include: (1) characterization of the EV charging behavior by clustering the arrival and departure time combinations that leads to the identification of type of EV charging behavior, (2) in-depth analysis of the characteristics of the charging sessions in each behavioral cluster and investigation of the influence of weekdays and seasonal changes on those characteristics including arrival, sojourn and idle times, and (3) proposing measures and an algorithm to quantitatively analyze how much flexibility (in terms of duration and amount) is used at various times of a day, for two representative scenarios. Understanding the characteristics of that flexibility (e.g., amount, time and duration of availability) and when it is used (in terms of both duration and amount) helps to develop more realistic price and incentive schemes in DR algorithms to efficiently exploit the offered flexibility or to estimate when to stimulate additional flexibility. (C) 2017 Elsevier Ltd. All rights reserved

Conversation and critique within the architectural design process: a linkograph analysis

Conversation and critique are central to architectural design practice as they function as tools for probing and further improving design ideas. We study the kind of design activities that take place in such conversation and critique within the architectural design process. We use linkographs to characterise the design process taking place during conversation. More precisely, we study conversations between design teachers and design students. In this article, an example design process is considered that takes place via a traditional face-to-face meeting. Using the resulting linkograph, we are able to assess the kind of design activity taking place during such sessions of conversation and critique

The first comprehensive LC–MS/MS method allowing dissection of the thiamine pathway in plants

Arabidopsis thaliana serves as a model plant for genetic research, including vitamin research. When aiming at engineering the thiamine (vitamin B1) pathway in plants, the availability of tools that allow the quantitative determination of different intermediates in the biosynthesis pathway is of pivotal importance. This is a challenge, given the nature of the compounds and the minute quantities of genetically engineered material that may be available for analysis. Here, we report on the first LC–MS/MS method for the simultaneous quantification of thiamine, its mono- and diphosphate derivatives and its precursors 4-methyl-5-(2-hydroxyethyl) thiazole (HET) and 4-amino-2-methyl-5-hydroxymethylpyrimidine (HMP). This method was optimized and validated for the quantitative determination of these analytes in Arabidopsis thaliana. All analytes were chromatographically separated within less than 2.5 min during an 8 min run. No unacceptable interferences were found. The method was fully validated based on international guidelines. Accuracy (%bias) and total imprecision (%CV) were within preset acceptance criteria for all analytes in both QC and real samples. All analytes were stable in extracted samples when stored for 48 h at 4 °C (autosampler stability) and when reanalyzed after storage at −80 °C and −20 °C for 2 weeks (freeze/thaw stability). We demonstrated the start material should be stored at −80 °C to ensure stability of all analytes during short- and long-term storage (up to 3 months). The validity and applicability of the developed procedure was demonstrated via its successful application on Arabidopsis lines, genetically engineered to enhance thiamine content