Physiological responses of C4 grasses to prolonged heat stress

C4 grasses are best adapted to the transition, warm-arid, and warm-humid climatic zones and have the ability to acquire thermotolerance by exposure to acute heat stress. Exposure to sub-lethal temperatures results in changes in physiological, biochemical, metabolic, and molecular processes. The response of two warm-season grasses to prolonged heat stress was investigated. Plants of hybrid bermudagrass (Cynodon dactylon × C. transvaalensis ‘Tifway’) and Japanese lawn grass (Zoysia japonica Steud. ‘Meyer’) were exposed for 168 h to supraoptimal temperature conditions (47°C) in controlled-environment chamber. Compared with zoysiagrass, bermudagrass showed greater damage. Metabolite profi les were affected by prolonged heat exposure, with signifi cant differences between these species. Consistent differences were found in total soluble sugars accumulation over the study period and severity of plant organ senescence. Bermudagrass roots were more affected, as compared to leaves. Leaf proteins expression determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis showed an early degradation in zoysiagrass, as thermal exposure proceeded. A signifi - cant net decline in protein content was observed after 48 h of exposure, while in bermudagrass an analogous decline was not detected until 96 h of treatment. Although heat stress is not considered a detrimental factor to C4 grass species, the two species showed signifi cant differences in their physiological response to continuous high temperatures

Winter Colour Retention and Spring Green-Up of Zoysiagrass Genotypes in Southern Europe

Zoysiagrass (Zoysia spp. Wild.) is a sustainable choice for the Mediterranean area, being identified as grass species adapted to low-input environments (limited water requirements, no fertilizer or pesticides after establishment). A distinct drawback in the transition zone is winter dormancy, a temporary suspension of visible growth, followed by discoloration and loss of functionality. In order to investigate the genotype/species effect in relation with agro-meteorological conditions, 10 cultivars/experimental lines were screened for their ability to extend the winter colour retention. A two years field study was conducted on mature swards and evaluated during the onset of dormancy and spring green-up using digital image analysis. For both parameters, in both years, the Authors observed a wide range of cultivar responses. Zoysia japonica genotypes in our study provided significantly shorter dormancy period than fine-leaved genotypes. In some instances, there were as much as 35 and 20 d difference between entries in respect to the winter colour retention and spring onset, respectively. The identification of genotypes with shorter dormancy than 'Meyer' (industry standard) may reduce use of cultural practices, as late-season nitrogen or iron application, normally used to extend the winter colour retention of zoysiagrass in late fall or early spring, showing different levels of success

Carbohydrate content, characterization and localization in bermudagrass stolons during establishment

Abstract Turfgrasses are continuously exposed to a wide range of detrimental stresses, such as mowing, traffic, low or high temperatures, salinity, drought, UV, hypoxia etc. Plant responses to stimuli involve nearly every aspect of plant physiology and metabolism. Carbohydrates, primary sucrose and starch, as principal metabolic products of photosynthesis, are stored in bermudagrass (Cynodon spp.) in stolons and rhizomes. Total nonstructural carbohydrates (TNC) levels have been used as indicators of health and physiological status in bermudagrass. TNC levels vary during the year and are weakly affected by N source. Establishment is a critical phase of bermudagrass management. The objectives of this research were to assess and characterize stolon carbohydrate content in nine bermudagrass cultivars during the full establishment phase in relation to establishment rates. Morphological and growth analyses showed distinct properties among the cultivars selected for this study. Significant differences in turf co..

Carbohydrate Metabolism During Wintering Period in Four Zoysiagrass Genotypes

A key barrier to widespread use of warm-season grasses in the transition zone between the temperate and subtropical climates is represented by the winter dormancy, a temporary suspension of visible growth of any plant structure including the meristem. In this environment, species with different photosynthetic carbon cycle coexist, leading to asynchrony in growth among warm- and cool-season grasses. The objectives of the present study were (i) to assess the carbohydrate content in four representative zoysiagrasses Zoysia japonica 'El Toro', 'Meyer', Z. pacifica, and Z. matrella 'Zeon' during three successive phases of vegetation phenology, senescence, dormancy, and green-up, and (ii) to characterize the metabolic responses to phenological variations under natural acclimation. Japanese lawn grass genotypes 'El Toro' and 'Meyer' showed longer dormancy periods than fine-leaved Z. pacifica and 'Zeon'. In Japanese lawn grass genotypes, the progressive decline of photosynthetic pigments was similar to that observed in the color retention. Over the experimental period, a significantly greater amount of starch reserves was observed in sprigs (horizontal stems) in the Japanese lawn grasses than in the fine-leaved zoysiagrasses. In general, total soluble sugars (TSS) in leaves and sprigs did not show evidence of sugar starvation during the senescence phase. TSS increased significantly during cold acclimation, resulting in a higher metabolic activity at the onset of green-up to support spring regreening in all zoysiagrasses except 'Meyer' leaves

Photosynthetic and Growth Responses of Arundo donax L. Plantlets Under Different Oxygen Deficiency Stresses and Reoxygenation

Promotion of nonfood species production to marginal, degraded lands abandoned by mainstream agriculture is affected by extremes of water availability (droughts and floods), which have increased in frequency and intensity and account for severe yield reduction. Arundo donax L., known as giant cane or giant reed, spontaneously grows in different kinds of environments with limitation to low temperature and is thus widespread in temperate and hot areas around the world. Moreover, this perennial rhizomatous grass has been recognized as a leading candidate crop in the Mediterranean for lignocellulosic feedstock due to its high C3 photosynthetic capacity, positive energy balance and low agroecological management demand. In this study, the photosynthetic performance and growth response of A. donax to waterlogging and submergence stress following a time course as well as their respective re-oxygenation were analyzed under reproducible and controlled environment conditions. Results of growth response showed that biomass production was strongly conditioned by the availability of oxygen. In fact, only waterlogged plants showed similar growth capacity to those under control conditions, while plants under submergence resulted in a dramatic reduction of this trait. The simultaneous measurements of both gas exchanges and chlorophyll fluorescence highlighted an alteration of both stomatal and non-stomatal photosynthetic behaviors during a short/medium period of oxygen deprivation and re-oxygenation. Photosynthetic CO2 uptake was strictly related to a combination of stomatal and mesophyll diffusional constrains, depending on the severity of the treatment and exposure time. Conditions of waterlogging and hypoxia revealed a slight growth plasticity of the species in response to prolonged stress conditions, followed by a fast recovery upon reoxygenation. Moreover, the rapid restoration of physiological functions after O2 deprivation testifies to the environmental plasticity of this species, although prolonged O2 shortage proved detrimental to A. donax by hampering growth and photosynthetic CO2 uptake

Seedling Establishment of Tall Fescue Exposed to Long-Term Starvation Stress

In germinating seeds under unfavorable environmental conditions, the mobilization of stores in the cotyledons is delayed, which may result in a different modulation of carbohydrates balance and a decrease in seedling vigor. Tall fescue (Festuca arundinacea Schreb.) caryopses grown at 4˚C in the dark for an extended period in complete absence of nutrients, showed an unexpected ability to survive. Seedlings grown at 4˚C for 210 days were morphologically identical to seedlings grown at 23˚C for 21 days. After 400 days, seedlings grown at 4˚C were able to differentiate plastids to chloroplast in just few days once transferred to the light and 23˚C. Tall fescue exposed to prolonged period at 4˚C showed marked anatomical changes: cell wall thickening, undifferentiated plastids, more root hairs and less xylem lignification. Physiological modifications were also observed, in particular related to sugar content, GA and ABA levels and amylolytic enzymes pattern. The phytohormones profiles exhibited at 4 and 23˚C were comparable when normalized to the respective physiological states. Both the onset and the completion of germination were linked to GA and ABA levels, as well as to the ratio between these two hormones. All plants showed a sharp decline in carbohydrate content, with a consequent onset of gradual sugar starvation. This explained the slowed then full arrest in growth under both treatment regimes. The analysis of amylolytic activity showed that Ca2+ played a central role in the stabilization of several isoforms. Overall, convergence of starvation and hormone signals meet in crosstalk to regulate germination, growth and development in tall fescue

The positive role of steviol glycosides in stevia (Stevia rebaudiana Bertoni) under drought stress condition

Steviol glycosides (SVglys) are a group of diterpenoids mainly present in the leaves of stevia (Stevia rebaudiana Bertoni). An experiment was conducted to find the functional role of SVglys compounds in stevia affected by drought stress. In this study, a liquid blend of SVglys (200 ppm) was sprayed on stevia plants grown in well-watered (90% field capacity) and drought-stress conditions (45% field capacity) and then the morphological traits and metabolites were evaluated. It was observed that leaf losses caused by drought stress were stopped through external application of SVglys and consequently the harvest index of stevia was increased. Metabolite analysis of stevia leaves showed that the total SVglys content was significantly decreased due to drought stress, but was compensated by external application of SVglys. Among the SVglys, Rebaudioside A responded more to external SVglys. A slight promotion in total antioxidant activity of stevia leaves was observed when external SVglys was applied. The glucose availability in stevia leaves was increased by external application of SVglys but only in well-watered plants. According to our findings, it can be concluded that in stevia, SVglys may have a positive function in drought stress tolerance by exerting a protective role under such conditions

Plant growth retardants (PGRs) affect growth and secondary metabolite biosynthesis in Stevia rebaudiana Bertoni under drought stress

Abstract Beyond the inhibitory action against the gibberellin biosynthesis, some plant growth retardants (PGRs) can play an important role in regulating plant responses to abiotic stress through the induction of different tolerance mechanisms. The aim of the present study was the exploitation of the potential of PGRs in enhancing the resistance to drought stress in Stevia rebaudiana Bert. Therefore, the effects of three PGRs on stevia plants grown under drought stress condition were investigated. Stevia plants were first subjected to water stress and, second, treated with PGRs to detect PGRs effect on biometric, productive and phytochemical characteristics of drought stressed-plants. The control plants were uniformly irrigated at 3-day intervals, while water-stress conditions were imposed by watering the plants at 12-day intervals. Subsequently, the Chlorocholine chloride (CCC, as Copalyl diphosphate synthase inhibitor and Kaurene synthase inhibitor), Paclobutrazol (PBZ, as Kaurene oxidase inhibitor) and Daminozide (DAM, as anti-gibberellins) were applied in drought stressed-plants. The CCC and DAM were sprayed on stevia shoots, while PBZ was drenched. The obtained results showed that leaf dry weight of stevia plants was significantly reduced by drought stress, but this parameter increased as a consequence of CCC and PBZ treatments. Drought stress also caused a significant reduction in total steviol glycoside (SVglys) content. This reduction was more pronounced in drought stressed-plants treated with CCC, while PBZ was able to counteract the SVglys reduction, with SVgly content similar to that observed in the control. Similarly, PBZ was able to increase the soluble sugar production and total antioxidant capacity in the leaves of stressed-stevia plants. These findings suggested that CCC and, in particular, PBZ had a protective effect on stevia growth under drought stress by induction of antioxidant defenses and soluble sugar production. CCC seems to inhibit gibberellin biosynthesis, preventing the SVglys production, while DAM and PBZ, as gibberellin inhibitors, didn't have a negative effect on SVglys production in drought stressed-plants. This observation seems to emphasize their role in limiting the rate of target enzymes of CCC in SVglys biosynthetic pathway. Moreover, the induction of glucose production, as a substrate for SVglys biosynthesis, could be a convincing evidence for SVglys promotion in PBZ treated-plants