Interferometer determinations of certain wave-lengths, between lambda 3770 and 4220, of the secondary spectrum of hydrogen

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A study of federal taxation in times of emergency.

Thesis (M.A.)--Boston Universit

A determination of the wave lengths of certain lines in the secondary spectrum of hydrogen

Thesis (M.A.)--Boston Universit

Finding human proteins that bind to a Lassa Virus protein

Viral hemorrhagic fevers are severe illnesses caused by many different viruses. Lassa Virus is one of these important pathogens in Western Africa, causing hemorrhagic fever and eventually death without early medical treatment. There is no vaccine and there is little information on host-pathogen interactions. Therefore, the interaction between viral proteins and host targets is useful to understand Lassa virus’s lifecycle and pathology, and to develop ways to prevent infection. In this project, we study the nucleoprotein of Lassa virus (NP), which has been reported to have anti-interferon (IFN) activity through elimination of double stranded RNA (dsRNA). These features could be shared with other hemorrhagic viruses which have proteins with similar structures. This is important to understand because conserved viral characteristics across biological systems could lead to broad spectrum antiviral therapies. In this study we used the yeast two-hybrid assay to identify viral-host cell protein interactions. This assay involves two proteins which create a functional transcription factor if they interact. This transcription factor will then allow for the expression of auxotrophic reporter genes. The NP gene was cloned into the yeast two-hybrid DNA binding domain plasmid, sequenced, and screened against libraries of human genes from liver, macrophages and interferon treated macrophages. We identified viral-host protein interactions that will give insight into host proteins targeted during Lassa infection. Similar interactions may be observed in Crimean Congo virus Nucleoprotein (N), which has a very similar structure. Additional studies in mammalian cells are needed to confirm interactions and to explore biochemical effects on viral protein functions

Bulge formed cooling channels with a variable lead helix on a hollow body of revolution

A method of constructing a nozzle having cooling channels comprises a shell and a liner which are formed into a body of revolution having an axis of revolution. Helical welds are formed to hold the liner and shell to each other with a channel position being defined between each pair of helical welds. Pressurized fluid which may be a gas or a liquid, is introduced between the weld pairs to outwardly bulge the material of at least one of the liner and shell to define the channels