Similarity Check: Subchronic toxicity test on combination of extracted Phyllanthus niruri and Centella asiatica on haematology in rats

Background: Meniran (Phyllathus niruri) and Pegagan (Centella asiatica) are well-known medicinal plants in Indonesia. Uses of Meniran and Pegagan as herbal medicines need to be examined for their subchronic activity to assure their safety. Objective: This study aimed to evaluate subchronic effects of a combination of extracted Meniran and Pegagan on Wistar rats through haematological parameters (erythrocytes, haemoglobin, haematocrit, MCV, MCH, and MCHC). Methods: Animal models used were 56 male and female Wistar rats and were divided randomly into 4 groups. Group 1 received CMC-Na 1% (control group). Group 2 received extracts of Meniran and Pegagan (50:50 mg/kgBW). Group 3 received extracts of Meniran and Pegagan (250:250 mg/kgBW). Group 4 received extracts of Meniran and Pegagan (1250:1250 mg/kgBW). Subchronic test of Meniran and Pegagan was conducted by providing treatments for the Wistar rats for 28 days. Their haematological substances were analysed statistically by using one way ANOVA and Kruskal-Wallis method with confidence interval of 95% and post-hoc. Results: This study found that the haematological substances in male Wistar rats were normal and did not significantly change (p>0,05). It also showed that haemoglobin and haematocrit substances in female rats were normal and did not significantly change (p>0,05). Erythrocytes, MCV, MCH, and MCHC in female rats indicated significant change (p<0,05), but they were still in normal ranges. Conclusion: It could be concluded that administration of the combination of extracte

The Sub-chronic Toxicity Test of Meniran (Phyllanthus niruri L.) and Pegagan (Centella asiatica) Extract in Wistar Strain Rats on Liver and Kidney Function

Meniran (Phyllantus niruri L.) and Pegagan (Centella asiatica L.) was proven had activity that considered as an antioxidant. However, the safety was not confirmed. This research is intended to evaluate sub-chronic toxicity of standardized combination extract of meniran and pegagan on liver and kidney function. This research used post-test-control design. Twenty female and twenty male Wistar strain rats divided into 4 groups. The first group was controlled in dosage of CMC Na 0.5%. The others were given a combination of meniran and pegagan extract in ratio with different dosage (50:50; 250:250; and 1250:1250 mg/KgBW). Sub-chronic toxicity test of meniran and pegagan combination was given orally once a day for 28 consecutive days. On the 29th day, all the rats were sacrificed and blood samples were analyzed using automatic analyzer. SGOT, SGPT, BUN, and creatinine value were statistically analyzed using one way ANOVA with post hoc LSD (p0.05). The results show that treatment of meniran and gotu kola combination had no significantly different of SGOT and creatinine value of male and female rats, and SGPT value of male rats (p0.05). The treatment of meniran and gotu kola combination had significantly different of BUN value of male and female rats, and SGPT value of female rats (p0.05) but still within normal range. Based on this study, it can be concluded that orally administered of meniran and gotu kola extract combination at doses 50:50 mg/KgBW; 250:250 mg/KgBW; and 1250:1250 mg/KgBW had no affected on SGOT, SGPT, BUN and creatinine value of Wistar strain rats on sub-chronic administration for 28 consecutive days