Loss of heterozygosity (LOH), malignancy grade and clonality in microdissected prostate cancer

The aim of the present study was to find out whether increasing malignancy of prostate carcinoma correlates with an overall increase of loss of heterozygosity (LOH), and whether LOH typing of microdissected tumour areas can help to distinguish between multifocal or clonal tumour development. In 47 carcinomas analysed at 25 chromosomal loci, the overall LOH rate was found to be significantly lower in grade 1 areas (2.2%) compared with grade 2 (9.4%) and grade 3 areas (8.3%, P = 0.007). A similar tendency was found for the mean fractional allele loss (FAL, 0.043 for grade 1, 0.2 for grade 2 and 0.23 for grade 3, P = 0.0004). Of 20 tumours (65%) with LOH in several microdissected areas, 13 had identical losses at 1–4 loci within two or three areas, suggesting clonal development of these areas. Markers near RB, DCC, BBC1, TP53 and at D13S325 (13q21–22) showed higher loss rates in grades 2 and 3 (between 25% and 44.4%) compared with grade 1 (0–6.6%). Tumour-suppressor genes (TSGs) near these loci might, thus, be important for tumour progression. TP53 mutations were detected in 27%, but BBC1 mutations in only 7%, of samples with LOH. Evaluation of all 25 loci in every tumour made evident that each prostate cancer has its own pattern of allelic losses. © 1999 Cancer Research Campaig

A Tale of Two Congresses: The Psychological Study of Psychical, Occult, and Religious Phenomena, 1900-1909

© 2014 Wiley Periodicals, Inc. In so far as researchers viewed psychical, occult, and religious phenomena as both objectively verifiable and resistant to extant scientific explanations, their study posed thorny issues for experimental psychologists. Controversies over the study of psychical and occult phenomena at the Fourth Congress of International Psychology (Paris, 1900) and religious phenomena at the Sixth (Geneva, 1909) raise the question of why the latter was accepted as a legitimate object of study, whereas the former was not. Comparison of the Congresses suggests that those interested in the study of religion were willing to forego the quest for objective evidence and focus on experience, whereas those most invested in psychical research were not. The shift in focus did not overcome many of the methodological difficulties. Sub-specialization formalized distinctions between psychical, religious, and pathological phenomena; obscured similarities; and undercut the nascent comparative study of unusual experiences that had emerged at the early Congresses

Taschenbuch für den Infanterieoffizier im Felde

herausgegeben von Friederich von HügelExlibrisstempel: "Militair-Bibliothek des Cantons Zürich" 002275167_0001 Exemplar der ETH-BIBExlibrisetikette: "Sucursregiment des Cantons Zürich" 011366166_0001 Exemplar der ETH-BI

Travels in Kashmir and the Panjab : containing a particular account of the government and character of the sikhs

from the German of Baron Charles Hügel ; with notes by T.B. Jervis ; published under the Patronage of the honourable the Court of Directors of the East India Compan

Toenail infection by Cladophialophora boppii

Cladophialophora boppii is a black yeast-like fungus that up to now has been only rarely described as a cause of human infection and whose role as a pathogen was not established despite its repeated isolation and genetic identification in these reports. Here we report the first case of a verified toenail infection caused by this fungus in a woman without any systemic disease or evidence of immunodeficiency. Identical dark molds were isolated from the same toenail at three points of time. Species identification was performed by scrutinizing the isolates morphologic, physiologic and genetic characteristics which resulted in their identification as Cladophialophora boppii. Oral treatment with terbinafin plus topical ciclopiroxolamine was effective. Read More: http://informahealthcare.com/doi/abs/10.3109/13693786.2010.51645