Laue gamma-ray lenses for space astrophysics:status and prospects

We review feasibility studies, technological developments and astrophysical prospects for Laue lenses devoted to hard X-/gamma-ray astronomy observations.Comment: 26 pages, 24 figures. Published in a special issue (Volume 2010 (2010), Article ID 215375, doi:10.1155/2010/215375) of the on-line journal "X-Ray Optics and Instrumentation", devoted to "X-Ray Focusing: Techniques and Applications

Gamma-ray line emission from OB associations and young open clusters

OB associations and young open clusters constitute the most prolific nucleosynthesis sites in our Galaxy. The combined activity of stellar winds and core-collapse supernovae ejects significant amounts of freshly synthesised nuclei into the interstellar medium. Radioactive isotopes, such as 26Al or 60Fe, that have been co-produced in such events may eventually be observed by gamma-ray instruments through their characteristic decay-line signatures. In order to study such signatures and to derive constraints on the involved nucleosynthesis processes, we developed a multi-wavelength evolutionary synthesis model for massive star associations. We apply this model to associations and clusters in the well-studied Cygnus region for which we re-determined the stellar census based on photometric and spectroscopic data. In particular we study the relation between 1.809 MeV gamma-ray line emission and ionising flux, since the latter has turned out to provide an excellent tracer of the global galactic 1.809 MeV emission. We compare our model to COMPTEL 1.8 MeV gamma-ray line observations from which we derive limits on the relative contributions from massive stars and core-collapse supernovae to the actual 26Al content in this region. Based on our model we make predictions about the expected 26Al and 60Fe line signatures in the Cygnus region. These predictions make the Cygnus region a prime target for the future INTEGRAL mission.Comment: 4 pages, 1 figure, Proc. 5th Compton Symposiu

Developing a second generation Laue lens prototype: high reflectivity crystals and accurate assembly

Laue lenses are an emerging technology that will enhance gamma-ray telescope sensitivity by one to two orders of magnitude in selected energy bands of the \sim 100 keV to \sim 1.5 MeV range. This optic would be particularly well adapted to the observation of faint gamma ray lines, as required for the study of Supernovae and Galactic positron annihilation. It could also prove very useful for the study of hard X-ray tails from a variety of compact objects, especially making a difference by providing sufficient sensitivity for polarization to be measured by the focal plane detector. Our group has been addressing the two key issues relevant to improve performance with respect to the first generation of Laue lens prototypes: obtaining large numbers of efficient crystals and developing a method to fix them with accurate orientation and dense packing factor onto a substrate. We present preliminary results of an on-going study aiming to enable a large number of crystals suitable for diffraction at energies above 500 keV. In addition, we show the first results of the Laue lens prototype assembled using our beamline at SSL/UC Berkeley, which demonstrates our ability to orient and glue crystals with accuracy of a few arcsec, as required for an efficient Laue lens telescope.Comment: Published in the proceedings of the SPIE conference held in San Diego in August 201

Fresnel lenses for X-ray and Gamma-ray Astronomy

Phase Fresnel lenses have the same imaging properties as zone plates, but with the possibility of concentrating all of the incident power into the primary focus, increasing the maximum theoretical efficiency from 11% to close to 100%. For X-rays, and in particular for gamma-rays, large, diffraction-limited phase Fresnel lenses can be made relatively easily. The focal length is very long - for example up to a million kms. However, the correspondingly high `plate-scale' of the image means that the ultra-high (sub-micro-arc-second) angular resolution possible with a diffraction limited gamma-ray lens a few metres in diameter can be exploited with detectors having \~mm spatial resolution. The potential of such systems for ultra-high angular resolution astronomy, and for attaining the sensitivity improvements desperately needed for certain other studies, are reviewed and the advantages and disadvantages vis-a-vis alternative approaches are discussed. We report on reduced-scale 'proof-of-principle tests' which are planned and on mission studies of the implementation of a Fresnel telescope on a space mission with lens and detector on two spacecraft separated by one million km. Such a telescope would be capable of resolving emission from super-massive black holes on the scale of their event horizons and would have the sensitivity necessary to detect gamma-ray lines from distant supernovae. We show how diffractive/refractive optics leads to a continuum of possible system designs between filled aperture lenses and wideband interferometric arrays.Comment: To be published in "Proceedings of the SPIE conference 5168 : Optics for EUV, X-ray and Gamma-Ray Astronomy", San Diego, August 200

New Pedagogical Models Facilitated by Technology

This paper discusses the outcome of research conducted to assess the effectiveness of Work-Based Learning (WBL) from the perspective of stakeholders. WBL has increasingly become an area of interest for the higher education (HE) sector. It can support the personal and professional development of students who are already in work. The focus of the learning and development tends to be on the student’s workplace activities. Previous research has mainly considered only two stakeholder contexts, namely the learner and the academic institution. The significance of the study stems from extending the stakeholder contexts to include the employer and the professional body. The aim of this study was to assess the effectiveness of delivery of WBL from the perspective of a range of stakeholders including students, programme leaders (PLs), tutors, university support services, employers and representatives of professional bodies. Case study research methodology was adapted with mixed method research techniques for data capture and analysis using both qualitative and quantitative approaches. The study examined five (5) WBL programmes at Northumbria University in the UK. The three most influential factors in the effectiveness of WBL were found to be: quality, access and support. The contribution to new knowledge in WBL research is through a “Four-Pillar model” which has been developed to reflect the stakeholder contexts. Consideration of this model helps ensure WBL programmes cater for the current demands from the labour market. The findings of this study include factors which facilitate and/or obstruct the effective implementation of WBL programmes whilst identifying feasible strategies to overcome those challenges and share them with all stakeholders of WBL. Recommendations are made on resolving the identified issues and to extend and improve the effectiveness of WBL. Finally this paper looks at how these results could apply to encourage WBL uptake in a third world developing country like Sri Lanka where you are starting from a zero base. Sri Lanka is yet to embark on WBL formally although online distance learning is more of a reality

Incorporation of atmospheric 15NO2-nitrogen into free amino acids by Norway spruce Picea abies (L.) Karst.

During spring and autumn 1991, potted 6-yearold spruce trees (Picea abies (L.) Karst.) were fumigated with 60 nl1–1 15NO2 for 4 days under controlled conditions in constant light. Current and previous flush needles, the bark and the fine roots were analysed for total 15N content and incorporation of 15N into the -amino nitrogen of free amino acids. In addition, in vitro nitrate reductase activity and stomatal conductance of the needles were measured. Nitrate reductase activity was significantly higher in the needles of fumigated trees compared to control trees exposed to filtered air. With an average of 9.1% 15N, free glutamate was the pool with the most label. Taking into account the time-course of the labelling of this pool, this figure can be taken as an estimate of the minimum contribution of NO2 to the N nutrition of the needles. 15N-labelled amino acids were also detected in the bark and the roots, indicating export from the needles

Effect of glucose on assimilatory sulphate reduction in Arabidopsis thaliana roots

With the aim of analysing the relative importance of sugar supply and nitrogen nutrition for the regulation of sulphate assimilation, the regulation of adenosine 5′‐phosphosulphate reductase (APR), a key enzyme of sulphate reduction in plants, was studied. Glucose feeding experiments with Arabidopsis thaliana cultivated with and without a nitrogen source were performed. After a 38 h dark period, APR mRNA, protein, and enzymatic activity levels decreased dramatically in roots. The addition of 0.5% (w/v) glucose to the culture medium resulted in an increase of APR levels in roots (mRNA, protein and activity), comparable to those of plants kept under normal light conditions. Treatment of roots with d‐sorbitol or d‐mannitol did not increase APR activity, indicating that osmotic stress was not involved in APR regulation. The addition of O‐acetyl‐l‐serine (OAS) also quickly and transiently increased APR levels (mRNA, protein, and activity). Feeding plants with a combination of glucose and OAS resulted in a more than additive induction of APR activity. Contrary to nitrate reductase, APR was also increased by glucose in N‐deficient plants, indicating that this effect was independent of nitrate assimilation. [35S]‐sulphate feeding experiments showed that the addition of glucose to dark‐treated roots resulted in an increased incorporation of [35S] into thiols and proteins, which corresponded to the increased levels of APR activity. Under N‐deficient conditions, glucose also increased thiol labelling, but did not increase the incorporation of label into proteins. These results demonstrate that (i) exogenously supplied glucose can replace the function of photoassimilates in roots; (ii) APR is subject to co‐ordinated metabolic control by carbon metabolism; (iii) positive sugar signalling overrides negative signalling from nitrate assimilation in APR regulation. Furthermore, signals originating from nitrogen and carbon metabolism regulate APR synergisticall

Mimicking respiratory phosphorylation using purified enzymes

The enzymes of oxidative phosphorylation are a striking example of the functional association of multiple enzyme complexes, working together to form ATP from cellular reducing equivalents. These complexes, such as cytochrome c oxidase or the ATP synthase, are typically investigated individually and therefore, their functional interplay is not well understood. Here, we present methodology that allows the co-reconstitution of purified terminal oxidases and ATP synthases in synthetic liposomes. The enzymes are functionally coupled via proton translocation where upon addition of reducing equivalents the oxidase creates and maintains a transmembrane electrochemical proton gradient that energizes the synthesis of ATP by the F1F0 ATP synthase. The method has been tested with the ATP synthases from Escherichia coli and spinach chloroplasts, and with the quinol and cytochrome c oxidases from E. coli and Rhodobacter sphaeroides, respectively. Unlike in experiments with the ATP synthase reconstituted alone, the setup allows in vitro ATP synthesis under steady state conditions, with rates up to 90 ATP×s(-1)×enzyme(-1). We have also used the novel system to study the phenomenon of "mild uncoupling" as observed in mitochondria upon addition of low concentrations of ionophores (e.g. FCCP, SF6847) and the recoupling effect of 6-ketocholestanol. While we could reproduce the described effects, our data with the in vitro system does not support the idea of a direct interaction between a mitochondrial protein and the uncoupling agents as proposed earlier