Dwelling Quietly in the Rich Club: Brain Network Determinants of Slow Cortical Fluctuations

For more than a century, cerebral cartography has been driven by investigations of structural and morphological properties of the brain across spatial scales and the temporal/functional phenomena that emerge from these underlying features. The next era of brain mapping will be driven by studies that consider both of these components of brain organization simultaneously -- elucidating their interactions and dependencies. Using this guiding principle, we explored the origin of slowly fluctuating patterns of synchronization within the topological core of brain regions known as the rich club, implicated in the regulation of mood and introspection. We find that a constellation of densely interconnected regions that constitute the rich club (including the anterior insula, amygdala, and precuneus) play a central role in promoting a stable, dynamical core of spontaneous activity in the primate cortex. The slow time scales are well matched to the regulation of internal visceral states, corresponding to the somatic correlates of mood and anxiety. In contrast, the topology of the surrounding "feeder" cortical regions show unstable, rapidly fluctuating dynamics likely crucial for fast perceptual processes. We discuss these findings in relation to psychiatric disorders and the future of connectomics.Comment: 35 pages, 6 figure

Influence of Chronic Exposure to Treated Sewage Effluent on the Distribution of White Blood Cell Populations in Rainbow Trout (Oncorhynchus mykiss) Spleen

Impairment of immune function in aquatic animals has been proposed as a possible consequence of low-level contamination of surface waters with anthropogenic substances such as through the discharge of wastewater into rivers, lakes, and oceans. The study at hand investigated the effects of chronic (32 weeks) exposure to sewage treatment plant (STP) effluent on the prevalence and distribution of different leucocyte populations in spleen samples of rainbow trout (Oncorhynchus mykiss). To simulate an infection, fish were injected intraperitoneally (ip) with inactivated Aeromonas salmonicida salmonicida, 6 weeks prior to the termination of the experiment. Immunohistological analysis revealed a marked decrease in thrombocyte numbers, an increase of monocytes, altered distribution of B-cells, and higher surface immunoglobulin expression, as well as activation of MHC class II expression in the spleen after exposure to 15% (v/v) effluent. The most prominent finding of the present study, however, was the occurrence of intraplasmatic deposits or inclusions with strong autofluorescence in spleen sections from effluent-exposed trout. In addition to effluent effects, injection of trout with A. salmonicida stimulated infiltration of monocytes, increased staining intensity on thrombocytes, and enhanced MHC class I expression in larger leucocytes surrounding melanomacrophage centres. In general, the current study demonstrates a marked, potentially adverse effect of STP effluent on spleen leucocytes and on the integrity of spleen tissue. The observed response suggests a constant unspecific stimulation of different leucocyte populations and is reminiscent of chronic inflammatio

A First Look at the Nuclear Region of M31 with Chandra

We report on the first observation of the nuclear region of M31 with the Chandra X-ray Observatory. The nuclear source seen with the Einstein and ROSAT HRIs is resolved into five point sources. One of these sources is within 1'' of the M31 central super-massive black hole. As compared to the other point sources in M31, this nuclear source has an unusual x-ray spectrum. Based on the spatial coincidence we identify this source with the central black hole, and note that the unusual spectrum is a challenge to current theories. A bright transient is detected ~26'' to the west of the nucleus, which may be associated with a stellar mass black hole.Comment: Submitted to ApJ Letters, 4 pages, 4 figures. email: garcia,ssm,fap,wrf,jem,cjf, @head-cfa.harvard.ed

Distinct migratory and non-migratory ecotypes of an endemic New Zealand eleotrid (Gobiomorphus cotidianus) – implications for incipient speciation in island freshwater fish species

Background: Many postglacial lakes contain fish species with distinct ecomorphs. Similar evolutionary scenarios might be acting on evolutionarily young fish communities in lakes of remote islands. One process that drives diversification in island freshwater fish species is the colonization of depauperate freshwater environments by diadromous (migratory) taxa, which secondarily lose their migratory behaviour. The loss of migration limits dispersal and gene flow between distant populations, and, therefore, is expected to facilitate local morphological and genetic differentiation. To date, most studies have focused on interspecific relationships among migratory species and their non-migratory sister taxa. We hypothesize that the loss of migration facilitates intraspecific morphological, behavioural, and genetic differentiation between migratory and non-migratory populations of facultatively diadromous taxa, and, hence, incipient speciation of island freshwater fish species. Results: Microchemical analyses of otolith isotopes (Sr-88, Ba-137 and Ca-43) differentiated migratory and non-migratory stocks of the New Zealand endemic Gobiomorphus cotidianus McDowall (Eleotridae). Samples were taken from two rivers, one lake and two geographically-separated outgroup locations. Meristic analyses of oculoscapular lateral line canals documented a gradual reduction of these structures in the non-migratory populations. Amplified fragment length polymorphism (AFLP) fingerprints revealed considerable genetic isolation between migratory and non-migratory populations. Temporal differences in reproductive timing (migratory = winter spawners, non-migratory = summer spawners; as inferred from gonadosomatic indices) provide a prezygotic reproductive isolation mechanism between the two ecotypes. Conclusion: This study provides a holistic look at the role of diadromy in incipient speciation of island freshwater fish species. All four analytical approaches (otolith microchemistry, morphology, spawning timing, population genetics) yield congruent results, and provide clear and independent evidence for the existence of distinct migratory and non-migratory ecotypes within a river in a geographically confined range. The morphological changes within the non-migratory populations parallel interspecific patterns observed in all non-migratory New Zealand endemic Gobiomorphus species and other derived gobiid taxa, a pattern suggesting parallel evolution. This study indicates, for the first time, that distinct ecotypes of island freshwater fish species may be formed as a consequence of loss of migration and subsequent diversification. Therefore, if reproductive isolation persists, these processes may provide a mechanism to facilitate speciation

Evaluating the Sampling Design of a Long-Term Community-Based Estuary Monitoring Program

Community-based monitoring programs (CBMPs) are a cost-effective option to collect the long-term data required to effectively monitor estuaries. Data quality concerns have caused some CBMP datasets, which could fill knowledge gaps for aquatic ecosystems, to go unused. The Community Aquatic Monitoring Program (CAMP) is a CBMP that has collected littoral nekton assemblage data from estuaries in the southern Gulf of St. Lawrence since 2003. Concerns with the CAMP sampling design (station placement and numbers) have prevented decision-makers from using the data to inform estuary health assessments. This study tested if CAMP’s sampling design that accommodates volunteer participation provides similar information as a scientific sampling approach. Six CAMP stations and six stations selected using a stratified random design were sampled at ten estuaries. A permutational-MANOVA revealed nekton assemblages were generally not significantly different between the two sampling designs. The current six CAMP stations are sufficient to detect the larger differences in species abundances that may indicate differences in estuary condition. The predicted increase in precision (2%) with twelve stations is not substantive enough to warrant an increased sampling effort. CAMP’s scientific utility is not limited by station selection bias or numbers. Furthermore, well-designed CBMPs can produce comparable data to scientific studies.Department of Fisheries and Oceans’ Gulf Fisheries Centre|| Prince Edward Island estuaries||CAM

A Catalog of Transient X-ray Sources in M31

From October 1999 to August 2002, 45 transient X-ray sources were detected in M31 by Chandra and XMM-Newton. We have performed spectral analysis of all XMM-Newton and Chandra ACIS detections of these sources, as well as flux measurements of Chandra HRC detections. The result is absorption-corrected X-ray lightcurves for these sources covering this 2.8 year period, along with spectral parameters for several epochs of the outbursts of most of the transient sources. We supply a catalog of the locations, outburst dates, peak observed luminosities, decay time estimates, and spectral properties of the transient sources, and we discuss similarities with Galactic X-ray novae. Duty cycle estimates are possible for 8 of the transients and range from 40% to 2%; upper limits to the duty cycles are estimated for an additional 15 transients and cover a similar range. We find 5 transients which have rapid decay times and may be ultra-compact X-ray binaries. Spectra of three of the transients suggest they may be faint Galactic foreground sources. If even one is a foreground source, this suggests a surface density of faint transient X-ray sources of >~1 deg$^{-2}$.Comment: 63 pages, 22 figures, 3 tables, accepted for publication in Ap

Time-sequenced Multi-Radio-Frequency Observations of Cygnus X-3 in Flare

Multifrequency observations from the VLA, VLBA and OVRO Millimeter Array of a major radio outburst of Cygnus X-3 in 2001 September are presented, measuring the evolution of the spectrum of the source over three decades in frequency, over a period of six days. Following the peak of the flare, as the intensity declines the high-frequency spectrum at frequency nu steepens from nu^{-0.4} to nu^{-0.6}, after which the spectral index remains at this latter terminal value; a trend previously observed but hitherto not satisfactorily explained. VLBA observations, for the first time, track over several days the expansion of a sequence of knots whose initial diameters are approximately 8 milliarcseconds. The light-crossing time within these plasmons is of the same order as the time-scale over which the spectrum is observed to evolve. We contend that properly accounting for light-travel time effects in and between plasmons which are initially optically thick, but which after expansion become optically thin, explains the key features of the spectral evolution, for example the observed timescale. Using the VLBA images, we have directly measured for the first time the proper motions of individual knots, analysis of which shows a two-sided jet whose axis is precessing. The best-fit jet speed is roughly beta = 0.63 and the precession period is about 5 days, significantly lower than fitted for a previous flare. Extrapolation of the positions of the knots measured by the VLBA back to zero-separation shows this to occur approximately 2.5 days after the detection of the rise in flux density of Cygnus X-3.Comment: 23 pages, 10 figures, accepted by Ap

Inflammation Is Associated with Voriconazole Trough Concentrations

Voriconazole concentrations display a large variability, which cannot completely be explained by known factors. Inflammation may be a contributing factor, as inflammatory stimuli can change the activities and expression levels of cytochrome P450 isoenzymes. We explored the correlation between inflammation, reflected by C-reactive protein (CRP) concentrations, and voriconazole trough concentrations. A retrospective chart review of patients with at least one steady-state voriconazole trough concentration and a CRP concentration measured on the same day was performed. A total of 128 patients were included. A significantly (

Episodic diamond growth beneath the Kaapvaal Craton at Jwaneng Mine, Botswana

Important implications for the interior workings of the Earth can be drawn by studying diamonds and their inclusions. To better understand the timing and number of diamond forming events beneath the NW margin of the Kaapvaal Craton, a comprehensive reassessment of Jwaneng’s diamond populations has been undertaken. We report new inclusion abundance data from the visual examination of ~130,000 diamonds that validate the predominance of an eclogitic diamond suite (up to 88%) with on average 5% inclusion-bearing diamonds (with inclusions >10 μm in size). From this population, polished plates from 79 diamonds of eclogitic and peridotitic paragenesis have been studied with cathodoluminescence (CL) imaging and infrared spectroscopy (FTIR) traverses. The majority (80%) record major changes in N concentration and aggregation states, as well as sharp boundaries in the CL images of individual plates that are interpreted to demarcate discrete diamond growth events. In addition, bulk FTIR data have been acquired for 373 unpolished diamonds. Silicate inclusions sampled from distinct growth zones define 2 compositional groups of omphacites and pyrope-almandines associated with different N contents in their diamond hosts. These findings reinforce previous observations that at Jwaneng at least seven individual diamond forming events can be identified – 3 peridotitic and 4 eclogitic. The results demonstrate that detailed examination of diamond plates by CL imaging and FTIR traverses is necessary to unveil the complex history recorded in diamonds