Photolysis of caged calcium using a low-cost flash unit: efficacy analysis with a calcium selective electrode

Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in our laboratory. A 300 J short arc xenon flash lamp (Heimann) was mounted in an elliptical reflector and driven by a modified Metz 60 CT 4 photoflash unit up to 240 J input energy and 4 ms flash duration. A 20 microliters cuvette containing a test solution was placed in a complementary elliptical reflector. An ion selective calcium electrode was used to measure the free calcium concentration [Ca2+] before and after flash in test solutions containing 1.00 mM Nitr5 in combination with different added [Ca2+]s. Using this technique we estimated that 1 flash on 1.00 mM Nitr5 increased the free [Ca2+] from 10(-7) to 1.1 x 10(-5) M. When the added [Ca2+] was less than 2.3 x 10(-4) M, the used Nitr5 behaved as a strong calcium chelator because 23% of it was unloaded with calcium. It is concluded that a physiologically relevant change in free [Ca2+] can be evoked by photolysis of Nitr5 using a low cost (approximately $1500) xenon flash unit, and that ion selective Ca electrodes can be adequately used to monitor the resulting changes in [Ca2+]

Effect of partial urethral obstruction on force development of the guinea pig bladder

We created gradual partial urethral obstruction in 20 guinea pigs using silver jeweler's jump rings. After 4 or 8 weeks obstruction all animals underwent cystometry and were assigned to one of five urodynamic categories: normal, high pressure voiding, unstable, low compliance, or decompensated. After sacrifice, the contractile responses of bladder strips to electrical field stimulation of intramural nerves, direct electrical muscle stimulation, 0.1 mM carbachol, and high K + solution were sampled by computer for phase plot analysis. Following 8 weeks obstruction, the value of the phase plot parameter Fiso, indicative of the number of contractile muscle units, was reduced to 60% of the control response to nerve stimulation (P < 0.05) and to 77% of the control response to carbachol stimulation (P < 0.05). Parameter C, the slope of the phase plot (indicative of unit recruitment during force development), was unchanged for all forms of stimulation. Although in the latter case not statistically significant, obstruction affected responses to nerve and muscle stimulation similarly suggesting that muscle change may possibly be a common denominator of dysfunction. In view of the reduction in Fiso and the increase in bladder weight, instability may represent a more advanced form of dysfunction due to obstruction than high pressure voiding

A new algorithm in patients with elevated and/or rising prostate-specific antigen level, minor lower urinary tract symptoms, and negative multisite prostate biopsies

Patients with elevated and/or rising prostate-specific antigen (PSA), minor lower urinary tract symptoms (LUTS), and no evidence for prostate cancer on (multiple) extended prostate biopsies are a regularly encountered problem in urological practice. Even now, patients are seen with no objective explanation of this persistent elevated and/or rising PSA. So far, many strategic proposals have been elaborated and published to deal with this specific population including the use of different PSA derivates; applying different biopsy schemes—strategies—biopsy target imaging; diagnostic use of prostate cancer genes; and many more. In this review, we propose a new algorithm in which an urodynamic evaluation should be included since bladder outlet obstruction (BOO) can be expected. Once BOO is confirmed, a transurethral resection of the prostate (TURP) can be offered to these patients. This procedure will result in subjective and biochemical improvement and allows extensive histological examination. Current literature was reviewed with regard to this specific population. This research was performed using the commercially available Medline online search tools and applying the following search terms: “diagnostic TURP”; “elevated PSA”; and “prostate biopsy”. Furthermore, subsequent reference search was executed on retrieved articles

Therapeutic Approaches, to Restore or Augment Detrusor Contractility “Bladder Wrap Procedures”

When a urinary bladder is acontractile, a comprehensive approach to the problem is to try and bring new functional muscle to the bladder wall. To establish this a skeletal muscle can be transposed around the bladder wall. Several procedures have been attempted in the last three decades. In all these cases a major challenge is to achieve muscle functionality in order to allow bladder emptying at an appropriate moment. Therefore, the muscle has to be innervated and should be at a length adequate to allow contraction. The muscle should contract during voiding and not during other activities such as physical exercise. In this chapter we discuss the progress to date in developing bladder wrap surgery