Sameness and difference in delta planning

Triggered by an increased awareness of the possible effects of climate change, many deltaic regions around the world are undertaking planning initiatives to address the problems they expect to face in the future. Dutch delta planning knowledge and expertise figure prominently in some of these initiatives. We use this article to ask why this is so. What makes Dutch delta knowledge special, and how does it become generic enough to travel to other places? The pertinence of these questions stems from the realization that deltas do not pre-exist human interventions, but are as much the effect of different planning cultures, trajectories and objectives, as they are their cause. Through a discussion of some telling anecdotes of delta planning, our analysis shows that while the Dutchness of delta planning expertise is a powerful branding, this expertise can only travel through a conscious and simultaneous process of un-Dutching: by packaging and scientizing Dutch Delta planning to turn it into a more generic Adaptive Delta Management approach

Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition

MicroRNA (miRNA) sponges are transcripts with repeated miRNA antisense sequences that can sequester miRNAs from endogenous targets. MiRNA sponges are valuable tools for miRNA loss-of-function studies both in vitro and in vivo. We developed a fast and flexible method to generate miRNA sponges and tested their efficiency in various assays. Using a single directional ligation reaction we generated sponges with 10 or more miRNA binding sites. Luciferase and AGO2-immuno precipitation (IP) assays confirmed effective binding of the miRNAs to the sponges. Using a GFP competition assay we showed that miR-19 sponges with central mismatches in the miRNA binding sites are efficient miRNA inhibitors while sponges with perfect antisense binding sites are not. Quantification of miRNA sponge levels suggests that this is at least in part due to degradation of the perfect antisense sponge transcripts. Finally, we provide evidence that combined inhibition of miRNAs of the miR-17∼92 cluster results in a more effective growth inhibition as compared to inhibition of individual miRNAs. In conclusion, we describe and validate a method to rapidly generate miRNA sponges for miRNA loss-of-function studies