Supplementary Figure S1 - Timeline experimental protocol - JDS19932

Supplementary Figure S1. Timeline of the experimental protocol per batch of calves (2 batches, 24 calves each). For each treatment (6 calves per treatment), the adaptation period ranged from 6 to 9 weeks for pair 1 to 3 (i.e., paired according to BW). The second batch of calves had a higher BW from the start, therefore having a shorter adaptation period ranging from 5 to 8 weeks. The adaptation period was followed by 7 days in climate respiration chambers (pair-housed) to measure the recovery of 13C tracers in breath after intravenous administration of [13C]sodium bicarbonate and oral administration of [1-13C]octanoate and bacterial protein, as detailed for pair 1. Subsequently, calves were fitted with harnesses to which plastic bags were attached and housed individually in a pen for 7 days, to determine the fecal excretion curves of 3 orally administrated indigestible markers (i.e., Cr-NDF, Yb2O3, and Co-EDTA), as detailed for pair 3

Supplementary Figure S1 and S2 - JDS20782 - GreenFeed visits

Supplementary Figure S1. The average number of GreenFeed visits (per cow) over a 24 h period of the dairy cows receiving a grass silage-based diet (GS; green), a grass silage- and corn silage-mixed diet (GSCS; orange), and a corn silage-based diet (CS; blue) supplemented with a placebo (NOP0; circle as symbol) or with 60 mg 3-nitrooxypropanol / kg DM (NOP60; triangle as symbol).Supplementary Figure S2. The average number of GreenFeed visits (per cow) over a 24 h period of the dairy cows receiving a grass silage-based diet (GS; green), a grass silage- and corn silage-mixed diet (GSCS; orange), and a corn silage-based diet (CS; blue) supplemented with a placebo (NOP0; circle as symbol) or with 80 mg 3-nitrooxypropanol / kg DM (NOP80; triangle as symbol).THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Supplementary Figure S2 - typical excretion curves - JDS19932

Supplementary Figure S2. Typical excretion curves of individual calf observations. The measured concentrations of Co (upper panel), Yb (middle panel), and Cr (lower panel) in feces with the fitted curve using the generalized Michaelis-Menten equation, as proposed by López et al. (2000)

Supplementary Figure S2 - JDS20782 - GreenFeed visits NOP0 and NOP80

Supplementary Figure S2. The average number of GreenFeed visits (per cow) over a 24 h period of the dairy cows receiving a grass silage-based diet (GS; green), a grass silage- and corn silage-mixed diet (GSCS; orange), and a corn silage-based diet (CS; blue) supplemented with a placebo (NOP0; circle as symbol) or with 80 mg 3-nitrooxypropanol / kg DM (NOP80; triangle as symbol).THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Supplementary Figure S1 - JDS20782 - GreenFeed visits NOP0 and NOP60

Supplementary Figure S1. The average number of GreenFeed visits (per cow) over a 24 h period of the dairy cows receiving a grass silage-based diet (GS; green), a grass silage- and corn silage-mixed diet (GSCS; orange), and a corn silage-based diet (CS; blue) supplemented with a placebo (NOP0; circle as symbol) or with 60 mg 3-nitrooxypropanol / kg DM (NOP60; triangle as symbol).THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Supplementary Figure S1 - JDS19933 - passage kinetics in veal calves

Supplementary Figure S1. Timeline of the experimental protocol per batch of calves (2 batches, 24 calves each). For each treatment (6 calves per treatment), the adaptation period ranged from 6 to 9 weeks for pair 1 to 3 (i.e., paired according to BW). The second batch of calves had a higher BW from the start, therefore having a shorter adaptation period ranging from 5 to 8 weeks. The adaptation period was followed by 7 days in climate respiration chambers (pair-housed) to measure the recovery of 13C tracers in breath after intravenous administration of [13C]sodium bicarbonate and oral administration of [1-13C]octanoate and bacterial protein, as detailed for pair 1. Subsequently, calves were fitted with harnesses to which plastic bags were attached and housed individually in a pen for 7 days, to determine the fecal excretion curves of 3 orally administrated indigestible markers (i.e., Cr-NDF, Yb2O3, and Co-EDTA), as detailed for pair 3

Supplementary Figure S2 - JDS19933 - passage kinetics in veal calves

Supplementary Figure S2. Typical excretion curves of individual calf observations. The measured concentrations of Co (upper panel), Yb (middle panel), and Cr (lower panel) in feces with the fitted curve using the generalized Michaelis-Menten equation, as proposed by López et al. (2000)

Supplementary Tables S1 and S2 - JDS20782 - Milk fatty acid composition

Supplementary Table S1. Milk fatty acid profile of lactating dairy cows fed different basal diets (BD; where GS = grass silage-based diet, GSCS = grass silage- and corn silage-mixed diet, CS = corn silage-based diet) supplemented with 0 or 60 mg 3-nitrooxypropanol/kg DM (NOP). Supplementary Table S2. Milk fatty acid profile of lactating dairy cows fed different basal diets (BD; where GS = grass silage-based diet, GSCS = grass silage- and corn silage-mixed diet, CS = corn silage-based diet) supplemented with 0 or 80 mg 3-nitrooxypropanol/kg DM (NOP).THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV