Using expert elicitation to abridge the Welfare Quality® protocol for monitoring the most adverse dairy cattle welfare impairments

The Welfare Quality® consortium has developed and proposed standard protocols for monitoring farm animal welfare. The uptake of the dairy cattle protocol has been below expectation, however, and it has been criticized for the variable quality of the welfare measures and for a limited number of measures having a disproportionally large effect on the integrated welfare categorization. Aiming for a wide uptake by the milk industry, we revised and simplified the Welfare Quality® protocol into a user-friendly tool for cost- and time-efficient on-farm monitoring of dairy cattle welfare with a minimal number of key animal-based measures that are aggregated into a continuous (and thus discriminative) welfare index (WI). The inevitable subjective decisions were based upon expert opinion, as considerable expertise about cattle welfare issues and about the interpretation, importance, and validity of the welfare measures was deemed essential. The WI is calculated as the sum of the severity score (i.e., how severely a welfare problem affects cow welfare) multiplied with the herd prevalence for each measure. The selection of measures (lameness, leanness, mortality, hairless patches, lesions/swellings, somatic cell count) and their severity scores were based on expert surveys (14–17 trained users of the Welfare Quality® cattle protocol). The prevalence of these welfare measures was assessed in 491 European herds. Experts allocated a welfare score (from 0 to 100) to 12 focus herds for which the prevalence of each welfare measure was benchmarked against all 491 herds. Quadratic models indicated a high correspondence between these subjective scores and the WI (R(2) = 0.91). The WI allows both numerical (0–100) as a qualitative (“not classified” to “excellent”) evaluation of welfare. Although it is sensitive to those welfare issues that most adversely affect cattle welfare (as identified by EFSA), the WI should be accompanied with a disclaimer that lists adverse or favorable effects that cannot be detected adequately by the current selection of measures

Relationship between Methods of Loading and Unloading, Carcass Bruising, and Animal Welfare in the Transportation of Extensively Reared Beef Cattle

In Uruguay, extensive, welfare-friendly beef production is a substantial part of the economy and culture. Transport of beef cattle to slaughterhouse compromises animal welfare. The objective of this study was to assess transport conditions related to carcass bruising. A total of 242 trucks with 8132 animals were assessed on loading, transport, unloading conditions, and carcass bruising. Average loading time was 26 min and 21 s and the perception of the truck drivers was correlated with the time took for loading and the use of devices. In 39.3% of the loadings only a flag was used. The average unloading time was 5 min and 54 s with a significant difference in time for the use of devices; only flag 3 min 51 s, cattle prod 6 min 43 s and sticks 8 min 09 s. Of the carcasses observed, 772 (9.5%) had no bruises, 873 (10.7%) had one bruise, 1312 (16.1%) two, 1231 (15.1%) three and 3944 (48.5%) had four or more bruises. Prevalence of bruises were highest on the Tuber-coxea (hip) (29.3%) following forequarter (22.4%), Tuber-ischiadicum (rear) (17.3%), ribs/flank (14.1%), rump/round (10.1%) and loin (6.8%). Bruises were 68.7% grade 1 and 31.3 % grade 2; there were no grade 3, the deepest ones, observed. It appeared that animal welfare training of truck drivers worked out well and the use of flags increased compared to a previous study in 2008

A New, Practical Animal Welfare Assessment for Dairy Farmers

The Welfare Quality® assessment protocol (WQ) is the most extensive way to measure animal welfare. This study was set up to determine if resource-based welfare indicators, that are easier and faster to measure, could replace the more time consuming, animal-based measurements of the WQ. The WQ was applied on 60 dairy farms in the Netherlands, with good, moderate and poor welfare. The WQ protocol classified most farms (87%) as 'acceptable'. Several of the animal-based measures of WQ correlated well with measures in the environment. Using these correlations, an alternative welfare assessment protocol (new Welfare Monitor) was designed, which takes approximately 1.5 h for a farm with 100 dairy cows. Because the opinion of farmers about welfare assessment is important if one wants to improve conditions for the cows at a farm, another objective of this study was to evaluate the usefulness of the new Welfare Monitor for the farmer. Over two years, the farms were visited, and advice was given to improve the conditions at the farm. After the first welfare assessment and advice, farmers improved the conditions for their cows substantially. Farms where the category score had increased made more improvements on average than those that did not upgrade

Enhancement of Bovine oocyte maturation by leptin is accompanied by an upregulation in mRNA expression of leptin receptor isoforms in cumulus cells

In this study, the mechanisms of supposed leptin action on oocyte maturation were examined. Expression of leptin mRNA, as determined with RT-PCR, was present in oocytes but not in cumulus cells. The long isoform of the leptin receptor (ObR-L) was expressed exclusively in cumulus cells after 7 and 23 hr of maturation. In oocytes the expression of the short receptor isoform (ObR-S) and all the receptor isoforms combined (ObR-T) did not change during maturation, as determined by quantitative RT-PCR, but in cumulus cells there was a significant increase in ObR-S transcripts after 7 hr of maturation. To determine if leptin plays a role in resumption of meiosis, oocytes meiotically arrested by the connection of the cumulus to a piece of granulosa layer were exposed to leptin. After 23 hr of culture, the proportion of oocytes that had resumed meiosis did not differ from the control. Exposure of COCs to leptin (1,000 ng/ml) resulted after 17 hr of maturation in a smaller proportion of oocytes that was still in metaphase-I stage (M-I) compared to the control group. Fertilization of oocytes after maturation in the presence of leptin resulted in a larger proportion of embryos that had developed to the 8-cell stage on Day 5 compared to the control group and in more blastocysts on Day 8 of culture. It is concluded that leptin enhances meiotic maturation of bovine oocytes, and that this effect is cumulus cell-mediated

A New, Practical Animal Welfare Assessment for Dairy Farmers

The Welfare Quality® assessment protocol (WQ) is the most extensive way to measure animal welfare. This study was set up to determine if resource-based welfare indicators, that are easier and faster to measure, could replace the more time consuming, animal-based measurements of the WQ. The WQ was applied on 60 dairy farms in the Netherlands, with good, moderate and poor welfare. The WQ protocol classified most farms (87%) as ‘acceptable’. Several of the animal-based measures of WQ correlated well with measures in the environment. Using these correlations, an alternative welfare assessment protocol (new Welfare Monitor) was designed, which takes approximately 1.5 h for a farm with 100 dairy cows. Because the opinion of farmers about welfare assessment is important if one wants to improve conditions for the cows at a farm, another objective of this study was to evaluate the usefulness of the new Welfare Monitor for the farmer. Over two years, the farms were visited, and advice was given to improve the conditions at the farm. After the first welfare assessment and advice, farmers improved the conditions for their cows substantially. Farms where the category score had increased made more improvements on average than those that did not upgrade

Evaluation of the Thermal Response of the Horns in Dairy Cattle

Dairy cattle are typically disbudded or dehorned. Little is known, however, about the biological function and role of horns during thermoregulatory processes in cattle, and thus about the potential physiological consequences of horn removal. Anecdotal evidence suggests that dairy cow horns increase in temperature during rumination, and few studies on other bovid species indicate that horns aid thermoregulation. The objective of this study was, therefore, to elucidate a possible thermoregulatory function of the horns in dairy cattle. Using non-invasive infrared thermography, we measured the superficial temperature of the horns, eyes, and ears of 18 focal cows on three different farms in a temperate climate zone under various environmental circumstances. Observations of social and non-social behaviours were conducted as well. Based on environmental temperature, humidity, and wind speed, the heat load index (HLI) was calculated as a measure of the heat load experienced by a cow. The temperature of the horns increased by 0.18 °C per unit HLI, indicating that horns serve the dissipation of heat. Dehorned cows had higher eye temperatures than horned cows, though this result should be interpreted with caution as the low sample size and experimental setup prevent casual conclusions. We did not, however, find changes in horn temperature during rumination, nor with any other behaviours. Our study thus supports a role of horns in thermoregulation, but not related to rumination. These results should be considered when assessing the potential consequences of horn removal, a painful procedure

Benefits of Silvopastoral Systems for Keeping Beef Cattle

The potential benefits of keeping Zebu cattle in silvopastoral systems are well described in tropical regions. In order to obtain information on European breeds of beef cattle (Bos taurus taurus) in temperate climate zones, individual records of body weight and welfare indicators were obtained from 130 beef cattle. These belonged to four herds and were randomly allocated to two contiguous plots: Silvopastoral Systems (SPS) and Open Pastures Systems (OPS). The SPS in this study were areas with exotic trees of Eucalyptus globulus globulus for paper pulp production planted in a 2 × 2 design (two meters between each tree) over diverse, native grasses. The OPS were large open areas with a great diversity of native grasses, herbs, and small plots of trees where the animals could rest and shelter from extreme weather conditions. Over the course of one year, individual body weights and a number of specific animal welfare indicators were measured every 45 days. After a descriptive analysis, a generalized linear mixed model (GLMM) with a Gaussian distribution, with time and system (OPS or SPS) fitted as fixed effects and individuals nested by herd as random intercepts, was used. The results showed that weight gain did not differ between the two systems. None of the animals showed any sign of impaired welfare in either system over the study period. Silvopastoral systems offer animals a sustainable and richer environment that will improves their welfare. The additional income provided by the wood production allows the farmers to maintain their traditional cattle farming lifestyle

Effect of ionization, bedding, and feeding on air quality in a horse stable

BACKGROUND: Organic dust is associated with Equine asthma. Ionization should reduce airborne dust levels. OBJECTIVES: To determine the effect of ionization of air, type of bedding, and feed on the levels of airborne dust, endotoxin, and fungal colonies in horse stables. ANIMALS: 24 healthy University-owned horses occupied the stables. METHODS: A randomized controlled cross-over study. Four units with 6 stables were equipped with an ionization installation (25 VA, 5000 Volt Direct Current). Horses were kept either on wood shavings and fed haylage (2 units), or on straw and fed dry hay (2 units). Measurements were performed with and without activated ionization, during daytime and nighttime, repeatedly over the course of a week and repeatedly during 4-6 weeks. Statistical analysis was performed using a mixed effect model with Akaike's Information Criterion for model reduction and 95% profile (log) likelihood confidence intervals (CI). RESULTS: Ionization did not alter concentrations of dust, endotoxin, or fungi, fewer. In the units with straw and hay, the concentration of dust, endotoxin, and fungi (difference in logarithmic mean 1.92 (95%CI 1.71-2.12); 2.86 (95%CI 2.59-3.14); 1.75 (95%CI 1.13-2.36)) were significantly higher compared to wood shavings and haylage. CONCLUSIONS AND CLINICAL IMPORTANCE: The installation of a negative air-ionizer in the horse stable did not reduce concentrations of dust, endotoxin, and viable fungal spores. The substantial effect of low dust bedding and feed is confirmed

Effect of ionization, bedding, and feeding on air quality in a horse stable

BACKGROUND: Organic dust is associated with Equine asthma. Ionization should reduce airborne dust levels. OBJECTIVES: To determine the effect of ionization of air, type of bedding, and feed on the levels of airborne dust, endotoxin, and fungal colonies in horse stables. ANIMALS: 24 healthy University-owned horses occupied the stables. METHODS: A randomized controlled cross-over study. Four units with 6 stables were equipped with an ionization installation (25 VA, 5000 Volt Direct Current). Horses were kept either on wood shavings and fed haylage (2 units), or on straw and fed dry hay (2 units). Measurements were performed with and without activated ionization, during daytime and nighttime, repeatedly over the course of a week and repeatedly during 4-6 weeks. Statistical analysis was performed using a mixed effect model with Akaike's Information Criterion for model reduction and 95% profile (log) likelihood confidence intervals (CI). RESULTS: Ionization did not alter concentrations of dust, endotoxin, or fungi, fewer. In the units with straw and hay, the concentration of dust, endotoxin, and fungi (difference in logarithmic mean 1.92 (95%CI 1.71-2.12); 2.86 (95%CI 2.59-3.14); 1.75 (95%CI 1.13-2.36)) were significantly higher compared to wood shavings and haylage. CONCLUSIONS AND CLINICAL IMPORTANCE: The installation of a negative air-ionizer in the horse stable did not reduce concentrations of dust, endotoxin, and viable fungal spores. The substantial effect of low dust bedding and feed is confirmed

Mutilating procedures, management practices, and housing conditions that may affect the welfare of farm animals : Implications for welfare research

A number of mutilating procedures, such as dehorning in cattle and goats and beak trimming in laying hens, are common in farm animal husbandry systems in an attempt to prevent or solve problems, such as injuries from horns or feather pecking. These procedures and other practices, such as early maternal separation, overcrowding, and barren housing conditions, raise concerns about animal welfare. Efforts to ensure or improve animal welfare involve adapting the animal to its environment, i.e., by selective breeding (e.g., by selecting "robust" animals) adapting the environment to the animal (e.g., by developing social housing systems in which aggressive encounters are reduced to a minimum), or both. We propose adapting the environment to the animals by improving management practices and housing conditions, and by abandoning mutilating procedures. This approach requires the active involvement of all stakeholders: veterinarians and animal scientists, the industrial farming sector, the food processing and supply chain, and consumers of animal-derived products. Although scientific evidence about the welfare effects of current practices in farming such as mutilating procedures, management practices, and housing conditions is steadily growing, the gain in knowledge needs a boost through more scientific research. Considering the huge number of animals whose welfare is affected, all possible effort must be made to improve their welfare as quickly as possible in order to ban welfare-compromising procedures and practices as soon as possible