Regulation of transferrin receptor synthesis by human cytotrophoblast cells in culture

The aim of this study was to examine the capacity of the syncytiotrophoblast to regulate transferrin receptor (TfR) synthesis in response to modulations in maternal iron supply. The model used was the primary trophoblast cell culture. Trophoblast cells isolated from term human placentas were cultured in iron-poor (Medium 199), iron-depleted (desferrioxamine (DFO)) and iron supplemented (diferric transferrin (hTf-2Fe), ferric ammonium citrate (FAG)) medium. TfR synthesis was reduced in response to hTf-2Fe supplementation. FAC did not modulate TfR synthesis. Iron deprivation by DFO resulted in clear stimulation of TfR synthesis. These results show that the differentiating trophoblast cells respond to pertubations in the (transferrin-mediated) iron supply by adjustments in the rate of TfR synthesis. Taking syncytiotrophoblast in culture as model for the maternal/fetal interface in vivo, our results would suggest that the placenta is able to make short term adjustments of the capacity for iron uptake

Biogeomorphology in the field: bedforms and species, a mystic relationship

Fine-scale seabed mapping (Owenia fusiformis, and the razor clam Ensis directus) occur near bedload convergence zones resulting from a mutually evasive flood- and ebb-dominant channel system. Such zones are at the end of the channels, hence also fine grained sediments, food and larvae are trapped. The combination of the coarser-grained bedload with thedeposition of fines is indeed the optimum for a lot of suspension and detritus feeders. Still, highest abundances occur at the fringes of such a system where stress levels are intermediate. Hypotheses were successfully tested along the Dutch coastal zone. Those insights are important to assess changes in seafloor integrity and hydrographic conditions

Application of multifractal wavelet analysis to spontaneous fermentation processes

An algorithm is presented here to get more detailed information, of mixed culture type, based exclusively on the biomass concentration data for fermentation processes. The analysis is performed with only the on-line measurements of the redox potential being available. It is a two-step procedure which includes an Artificial Neural Network (ANN) that relates the redox potential to the biomass concentrations in the first step. Next, a multifractal wavelet analysis is performed using the biomass estimates of the process. In this context, our results show that the redox potential is a valuable indicator of microorganism metabolic activity during the spontaneous fermentation. In this paper, the detailed design of the multifractal wavelet analysis is presented, as well as its direct experimental application at the laboratory levelComment: 12 pages, 3 figures, Physica A, to appea

Psychometric Properties of the Chinese Version of the Highly Sensitive Child Scale Across Age Groups, Gender, and Informants

Sensory Processing Sensitivity (SPS) is theorized to be a fundamental trait capturing children’s general sensitivity to the environment. Yet, scientific knowledge of SPS is mostly based on findings from Western cultures and few translated measures exist to assess children’s SPS outside of Western countries. Therefore, we developed the Chinese Highly Sensitive Child (HSC) scale. In Study 1, we investigated the scale’s psychometric properties for both self-reports (N = 2925, Mage = 11.74 years, 43.3% girls) and caregiver reports (n = 460, Mchild age = 9.02 years, 44.0% girls). Findings replicated most psychometric properties found in international studies including: (a) a bifactor structure with one general sensitivity component and three specific components, (b) acceptable internal consistency of the total scale (although not for self-report of elementary school children, and not for the subscales), and (c) at least partial invariance across age groups, gender, and informants. In Study 2, we investigated convergent validity with related temperament and personality measures using self-reports from both elementary school children (n = 845, Mage = 9.71 years, 41.9% girls) and middle school children (n = 563, Mage = 13.17 years, 43.2% girls). Findings replicated bivariate associations found in Western studies: Ease of Excitation (EOE) was associated with more positive traits, whereas Aesthetic Sensitivity (AES) was associated with more negative traits, suggesting that EOE and AES may capture the “dark” and “bright side” of sensitivity, respectively. We hope that our studies help spur research on SPS across western and Chinese cultures

Landbouwperspectieven in Noord-Holland tot 2040 : bouwstenen voor de structuurvisie van de provincie

Dit rapport is het resultaat van een onderzoek naar de landbouwstructuur in de provincie Noord-Holland. Het onderzoek was ondernomen in opdracht van het adviesbureau Decisio B.V., dat in opdracht van de provincie werkt. Doel was om bouwstenen aan te leveren voor de provinciale structuurvisie. In het kader van de nieuwe Wet ruimtelijke ordening moeten alle provincies een dergelijke structuurvisie maken. Het rapport beschrijft de huidige situatie en recente trends in de Noord-Hollandse landbouwsector, en de perspectieven voor de komende decennia. Het accent ligt daarbij op de behoefte aan ruimte, en hoe deze kan worden ingevuld binnen eisen van ruimtelijke kwaliteit. This report is the result of a study into the agricultural structure in the province of Noord-Holland. The study was undertaken on behalf of consultancy firm Decisio B.V., which works for the province. The aim was to supply building blocks for the provincial structural vision. In the framework of the new Spatial Planning Act, all provinces must produce a strong structural vision. The report describes the current situation and recent trends in the agricultural sector in Noord-Holland and the prospects for the coming decades. The emphasis here lies on the need for space and how this can be provided within demands for spatial quality

Human-water interface in hydrological modeling: Current status and future directions

Over the last decades, the global population has been rapidly increasing and human activities have altered terrestrial water fluxes at an unprecedented scale. The phenomenal growth of the human footprint has significantly modified hydrological processes in various ways (e.g., irrigation, artificial dams, and water diversion) and at various scales (from a watershed to the globe). During the early 1990s, awareness of the potential water scarcity led to the first detailed global water resource assessments. Shortly thereafter, in order to analyse the human perturbation on terrestrial water resources, the first generation of large-scale hydrological models (LHMs) was produced. However, at this early stage few models considered the interaction between terrestrial water fluxes and human activities, including water use and reservoir regulation, and even fewer models distinguished water use from surface water and groundwater resources. Since the early 2000s, a growing number of LHMs are incorporating human impacts on hydrological cycle, yet human representations in hydrological models remain challenging. In this paper we provide a synthesis of progress in the development and application of human impact modeling in LHMs. We highlight a number of key challenges and discuss possible improvements in order to better represent the human-water interface in hydrological models

Human–water interface in hydrological modelling: current status and future directions

Over recent decades, the global population has been rapidly increasing and human activities have altered terrestrial water fluxes to an unprecedented extent. The phenomenal growth of the human footprint has significantly modified hydrological processes in various ways (e.g. irrigation, artificial dams, and water diversion) and at various scales (from a watershed to the globe). During the early 1990s, awareness of the potential for increased water scarcity led to the first detailed global water resource assessments. Shortly thereafter, in order to analyse the human perturbation on terrestrial water resources, the first generation of largescale hydrological models (LHMs) was produced. However, at this early stage few models considered the interaction between terrestrial water fluxes and human activities, including water use and reservoir regulation, and even fewer models distinguished water use from surface water and groundwater resources. Since the early 2000s, a growing number of LHMs have incorporated human impacts on the hydrological cycle, yet the representation of human activities in hydrological models remains challenging. In this paper we provide a synthesis of progress in the development and application of human impact modelling in LHMs. We highlight a number of key challenges and discuss possible improvements in order to better represent the human-water interface in hydrological models

ADAM12 is a circulating marker for stromal activation in pancreatic cancer and predicts response to chemotherapy

Pancreatic ductal adenocarcinoma (PDAC) is characterized by abundant stroma that harbors tumor-promoting properties. No good biomarkers exist to monitor the effect of stromal targeting therapies or to predict response. We set out to identify such non-invasive markers for PDAC stroma and predict response to therapy. Gene expression datasets, co-culture experiments, xenografts, and patient samples were analyzed. Serum samples were measured from a cohort of 58 resected patients, and 87 metastatic or locally advanced PDAC patients. Baseline and follow-up levels were assessed in 372 additional metastatic PDAC patients who received nab-paclitaxel with gemcitabine (n = 184) or gemcitabine monotherapy (n = 188) in the phase III MPACT trial. Increased levels of ADAM12 were found in PDAC patients compared to healthy controls (p < 0.0001, n = 157 and n = 38). High levels of ADAM12 significantly associated with poor outcome in resected PDAC (HR 2.07, p = 0.04). In the MPACT trial survival was significantly longer for patients who received nab-paclitaxel and had undetectable ADAM12 levels before treatment (OS 12.3 m vs 7.9 m p = 0.0046). Consistently undetectable or decreased ADAM12 levels during treatment significantly associated with longer survival as well (OS 14.4 m and 11.2 m, respectively vs 8.3, p = 0.0054). We conclude that ADAM12 is a blood-borne proxy for stromal activation, the levels of which have prognostic significance and correlate with treatment benefit