Mechanics of cooling liquids by forced evaporation in bubbles

Injecting a non-dissolvable gas into a saturated liquid results in sub-cooling of the liquid due to forced evaporation into the bubble. Previous studies assumed the rate of evaporation of liquid into the bubble to be independent of the degree of sub-cooling. In our study we quantify the bubble growth by direct observation using high speed imaging and prove that this hypothesis is not true. A phenomenological model of the bubble growth as a function of the degree of sub-cooling is developed and we find excellent agreement between the measurements and theory. This bubble cooling process is employed in cooling a liquid. By identification of all heat flows, we can well describe the cool down curve using bubble cooling. Bubble cooling provides an alternative cooling method for liquids without the use of complicated cooling techniques

SupernetNL program: 3.4 km 110 kV AC underground superconducting cable in the Dutch grid

TenneT, a leading European electricity transmission system operator (TSO) is planning to install a 3.4 km long underground superconducting 110 kV cable as part of the Dutch electricity grid, in the city of Enschede. HTS cables have already been demonstrated on a relatively small scale in other countries, but they are usually not part of the meshed high-voltage grid and the length of the relevant cable section generally does not exceed 1 km. In 2009, a 600-meter section of HTS cable was installed in New York, and in 2014 a 1-km long section was taken in operation in Essen, Germany to replace a 10 kV AC medium-voltage line. In the Supernet NL program, TenneT is working together with several leading knowledge institutes including University of Twente, Delft University of Technology, the Institute of Science and Sustain- able Development (IWO), HAN University of Applied Sciences and RH Marine. These institutes have been investigating control engineering aspects and the requirements the cable must meet. In the meantime, the tender process has been started which consists of two phases. In the fi rst phase (summer 2017) appropriate candidates are selected directly followed by a call for tender in August. Receipt of the best and fi nal offer is scheduled for the end of November. In the presentation, the project will be introduced and requirements will be discussed, specifi cally focusing on the cryogenic aspects

Expression of Multiple Artificial MicroRNAs from a Chicken miRNA126-Based Lentiviral Vector

Background: The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector. Methods/Principal Findings: We have developed a novel chicken miR126-based artificial miRNA expression system that can express one, two or three miRNAs from a single cassette in a lentiviral vector. We show that each of the miRNAs expressed from the same lentiviral vector is capable of potent inhibition of reporter gene expression in transient transfection and stable integration assays in chicken fibroblast DF-1 cells. Transduction of Vero cells with lentivirus expressing two or three different anti-influenza miRNAs leads to inhibition of influenza virus production. In addition, the chicken miR126-based expression system effectively inhibits reporter gene expression in human, monkey, dog and mouse cells. These results demonstrate that the flanking regions of a single primary miRNA can support processing of three different stem-loops in a single vector. Conclusions/Significance: This novel design expands the means to express multiple miRNAs from the same vector for potent and effective silencing of target genes and influenza virus.National Institutes of Health (U.S.) (Grant R01AI056267)Cobb-Vantress, inc