Pemanfaatan Bahan Teknis Kno3, Cacl2, Mgso4, Kh2po4 Sebagai Hara Makro Dan Benzil Adenin Dalam Perbanyakan Jati (Tectona Grandis L) Secara in Vitro

Indonesia is a major producer of teak after India and will gradually decrease if not followed by replanting. In general, teak plants propagated through seeds but have many disadvantages. Teak plant propagation using in vitro technique being an alternative to get the mass production of teak clones. In vitro technique, to some extent, needs a high cost particularly in using pure chemical substance. As an alternative solution, pure chemical substance can be substituted by using a few technique chemical substances such as KNO3, CaCl2, MgSO4, and KH2PO4. The aim of this research was to evaluated induction of shoot teaks, planted on different media with macro element substituted by different chemicals such as KNO3, CaCl2, MgSO4, and KH2PO4. The result showed that using the same concentration between 2 different chemical substances on teak shoot induction, there was no different shoot growing in teak propagated between 2 media

Regenerasi Tanaman Obat Keladi Tikus (Thyponium Flagelliform L. Blume) Melalui Embriogenesis Somatik Secara in Vitro

Somatic embryogenesis is a process whereby somatic cells have differentiation in somatic embryos and it's formed from embryonic cells due to the high polarization of uncontrolled cell division. The aim of this research is to produced shoots of rodent tuber via somatic embryos.Induction of somatic embryos from explants in MS media with N (2-chloro-4-pyridyl)-Nphenyl urea (CPPU) and continuous subculture in MS media without plant growth regulator for 1 month. Plantlet regeneration growth in liquid MS media with BAP and IBA fortified with 40 gr/l of sucrose in the dark. The result showed that somatic embryonic appears in media induction after 2 week and plantlet regeneration in 4 week. All plantlet growed from embryosomatic can survive 100% in field with normal shoot