Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

AbstractCoat color is an important characteristic and economic trait in domestic sheep. Aiming at alteration of Chinese merino sheep coat color by genome manipulation, we disrupted sheep agouti signaling protein gene by CRISPR/Cas9. A total of seven indels were identified in 5 of 6 born lambs. Each targeted lamb happened at least two kinds of modifications, and targeted lambs with multiple modifications displayed variety of coat color patterns. Three lambs with 4 bp deletion showed badgerface with black body coat color in two lambs, and brown coat color with light ventral pigmentation in another one. The black-white spotted color was observed in two lambs with 2 bp deletion. Further analysis unraveled that modifications happened in one or more than two copies of ASIP gene, and moreover, the additional spontaneous mutations of D9 and/or D5 preceding the targeting modification could also involve the formation of coat color patterns. Taken together, the entanglement of ASIP modifications by CRISPR/Cas9, spontaneous D9/D5 mutations, and ASIP gene duplications contributed to the variety of coat color patterns in targeted lambs.</jats:p

Key issues for long-pulse high- β N operation with the Experimental Advanced Superconducting Tokamak (EAST)

In the last few years, long-pulse H-mode plasma discharges (with small edge-localized modes and normalized beta, β N ~ 1) have been realized at the Experimental Advanced Superconducting Tokamak (EAST). This paper reports on high-β N (>1.5) discharges in the 2015 EAST campaign. The characteristics of these H-mode plasmas have been presented in a database. Analysis of the experimental limit of β N has revealed several main features of typical discharges. Firstly, efficient, stable high heating power is required. Secondly, control of impurity radiation (partly due to interaction between the plasma and the in-vessel components) is also a critical issue for the maintenance of high-β N discharges. In addition an internal transport barrier (ITB) has recently been observed in EAST, introducing further improvement in confinement surpassing H-mode plasmas. ITB dynamics is another key issue for high-β N plasmas in EAST. Each of these features is discussed in this paper. Study and improvement of these issues could be considered as the key to achieving long-pulse high-β N operation with EAST