Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson's Disease

It has been shown that molecular hydrogen (H2) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD). Here, we show that drinking H2-containing water significantly reduced the loss of dopaminergic neurons in PD model mice using both acute and chronic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The concentration-dependency of H2 showed that H2 as low as 0.08 ppm had almost the same effect as saturated H2 water (1.5 ppm). MPTP-induced accumulation of cellular 8-oxoguanine (8-oxoG), a marker of DNA damage, and 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation were significantly decreased in the nigro-striatal dopaminergic pathway in mice drinking H2-containing water, whereas production of superoxide (O2•−) detected by intravascular injection of dihydroethidium (DHE) was not reduced significantly. Our results indicated that low concentration of H2 in drinking water can reduce oxidative stress in the brain. Thus, drinking H2-containing water may be useful in daily life to prevent or minimize the risk of life style-related oxidative stress and neurodegeneration

ホイクエン ノ キュウショク ト ホイク ノ レンケイ 2

　給食担当者と保育担当者が連携する．保育園の給食の場面を通して保育に食文化を取り入れること，それによって保育全体を豊かにしようとする研究の2 報である．　保育者集団の食に関する意識が必ずしも高まらない中で，一クラスの担任と給食が取り組んだ子どものお店体験・お店ごっこの実践の報告である．　お店体験により高まったイメージが日常の保育に行かされるようになった．　また給食担当者の課題意識は，保育担当者より以上に保育の基本にそったものであった．What would become of the situation at nursery school when the person in charge of lunch servicecollaborates with the person in charge of childcare? Here are two research reports on efforts to improve childcare in general by incorporating dietary culture into childcare through lunch served at nursery school. The reports include practical efforts made by a care provider and a lunch server who are in charge of the same class under the theme of Hands-on Shopkeeper/Playing Shopkeeper, a program for children. These efforts occur in a context where awareness among childcare workers about the eating habits of nursery school children is rather slow in growing. With the aim of applying an enhanced vision through Hands-on Shopkeeper Program to daily childcare, the lunch server and the care provider should share a common vision. The research revealed that those who were engaged in lunch service were more consciously sticking to the basics of childcare than care providers themselves

Antifreeze protein prolongs the life-time of insulinoma cells during hypothermic preservation.

It is sometimes desirable to preserve mammalian cells by hypothermia rather than freezing during short term transplantation. Here we found an ability of hypothermic (+4°C) preservation of fish antifreeze protein (AFP) against rat insulinoma cells denoted as RIN-5F. The preservation ability was compared between type I-III AFPs and antifreeze glycoprotein (AFGP), which could be recently mass-prepared by a developed technique utilizing the muscle homogenates, but not the blood serum, of cold-adapted fishes. For AFGP, whose molecular weight is distributed in the range from 2.6 to 34 kDa, only the proteins less than 10 kDa were examined. The viability rate was evaluated by counting of the preserved RIN-5F cells unstained with trypan blue. Significantly, either AFPI or AFPIII dissolved into Euro-Collins (EC) solution at a concentration of 10 mg/ml could preserve approximately 60% of the cells for 5 days at +4°C. The 5-day preserved RIN-5F cells retained the ability to secrete insulin. Only 2% of the cells were, however, preserved for 5 days without AFP. Confocal photomicroscopy experiments further showed the significant binding ability of AFP to the cell surface. These results suggest that fish AFP enables 5-day quality storage of the insulinoma cells collected from a donor without freezing

Simultaneous bilateral mastectomy and RRSO for BRCA2-positive non-invasive breast cancer in Japan: a case report and analysis of initial experience

Background: In Japan, genetic testing, surveillance, and risk-reducing surgery for hereditary breast and ovarian cancer (HBOC) syndrome have been covered by the Japanese national insurance system since April 2020. On the other hand, the current situation is that medical care, including surveillance of undiagnosed (cancer-free) patients, is self-funded even for individuals with HBOC. We report a case in which breast cancer was diagnosed at an early stage during surveillance for cancer-free HBOC at the patient’s own expense, and risk-reducing surgery was performed at the same time as treatment for breast cancer. Case presentation: The patient was a 63-year-old woman. Her sister had a history of breast cancer in her 30s and was found to be a BRCA2 pathogenic variant carrier by genetic testing. The patient therefore presented to the genetic department of our hospital and underwent genetic testing (out-of-pocket). A pathogenic variant was found at the same site. During annual breast and ovarian surveillance at the patient’s own expense, a physician with sufficient expertise in contrast-enhanced breast magnetic resonance imaging (MRI) noticed a change in the contrast enhancement pattern on breast MRI and performed needle biopsy, revealing ductal carcinoma in situ. At the request of the patient, she underwent concurrent contralateral risk-reducing mastectomy and risk-reducing salpingo-oophorectomy in addition to breast cancer treatment. Conclusions: We encountered a case in which cancer treatment and risk-reducing surgery were performed at the same time for a pathogenic variant carrier who was very anxious about developing cancer. Surveillance of cancer-free BRCA1/2 mutation carriers and expansion of insurance coverage for surgery are important future issue.Hereditary Cancer in Clinical Practice, 21(1), art. no. 23; 202

Time-dependent change in the survival rate (%) of rat insulinoma cell-line, RIN-5F, under hypothermic exposure (+4°C).

<p>A. The survival rate evaluated by counting the number of living cells (Fig. 1B). B. The survival rate evaluated by measuring the amount of insulin secreted from living cells (Fig. 1C). A freshly made Euro-Collins solution (EC) was used as a basic cell-preservation fluid to dissolve AFPI–III, trehalose, and BSA.</p

Analyses of purity and structural integrity of the AFP samples.

<p>A. Electrophoretogram of AFPI–III and AFGP samples provided by Nichirei Foods Inc. on 16.5% tricine-SDS-PAGE. The AFGP sample only contains molecules less than 10 kDa owing to a filtration step. B. The shape of an ice crystal and TH activity obtained for each AFP sample dissolved into the Euro-Collins solution. All protein concentrations were 10 mg/ml.</p

Confocal photomicroscope images of human hepatoma cells (HepG2) and rat insulinoma cells (RIN-5F) in Euro Collins-solutions containing 0.4 mg/ml of BSA (A), AFPIII (B and C), and AFPI (D) labeled with fluorescence.

<p>The images A and B show the slice data of 1 µm-width of HepG2, and A’ and B’ an intact cell images synthesized by stacking of the slices. These 4 images were reproduced from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073643#pone.0073643-Tsuda1" target="_blank">[22]</a> with permission. The images C and D are the slice data for RIN-5F cells. All the images were captured after 1 h-preservation at 37°C. Accumulation of AFPIII on the cell-surface is more evident compared with BSA.</p