13 research outputs found
In vitro activity of antibiotics against biofilms produced by MRSA SCCmec IV isolates from hospitals in Rio de Janeiro
First report of the blaOXA-58 gene in a clinical isolate of Acinetobacter baumannii in Rio de Janeiro, Brazil
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics
Accuracy of phenotypic methicillin susceptibility methods in the detection of Staphylococcus aureus isolates carrying different SCCmec types
Accuracy of phenotypic methicillin susceptibility methods in the detection of Staphylococcus aureus isolates carrying different SCCmec types
A total of 138 isolates, 118 methicillin-resistant Staphylococcus
aureus (MRSA) isolates (staphylococcal cassette chromosome type II, 20
isolates, type III, 39 isolates and type IV, 59 isolates) and 20
methicillin-sensitive S. aureus isolates were evaluated by phenotypic
methods: cefoxitin and oxacillin disk diffusion (DD), agar dilution
(AD), latex agglutination (LA), oxacillin agar screening (OAS) and
chromogenic agar detection. All methods showed 100% specificity, but
only the DD tests presented 100% sensitivity. The sensitivity of the
other tests ranged from 82.2% (OAS)-98.3% (AD). The LA test showed the
second lowest sensitivity (86.4%). The DD test showed high accuracy in
the detection of MRSA isolates, but there was low precision in the
detection of type IV isolates by the other tests, indicating that the
genotypic characteristics of the isolates should be considered
Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals
Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria
Bacteria isolated from marine sponges found off the coast of Rio de Janeiro, Brazil, were screened for the production of antimicrobial substances. We report a new Pseudomonas putida strain (designated P. putida Mm3) isolated from the sponge Mycale microsigmatosa that produces a powerful antimicrobial substance active against multidrug-resistant bacteria. P. putida Mm3 was identified on the basis of 16S rRNA gene sequencing and phenotypic tests. Molecular typing for Mm3 was performed by RAPD-PCR and comparison of the results to other Pseudomonas strains. Our results contribute to the search for new antimicrobial agents, an important strategy for developing alternative therapies to treat infections caused by multidrug-resistant bacteria