2 research outputs found
Prospective study of a Bothrops jararacussu venom batch (Bj2015) - phospholipase A(2) activity, immunogenicity, neurotoxicity, and myotoxicity parameters
Bothrops jararacussu venom's (Bj2015) batch was biomonitored quarterly for one year to assess phospholipase A(2) (PLA(2)) activity, immunogenicity, neurotoxicity, and myotoxicity. In silico models were applied to evaluate losses using decay model and recoveries by predictive trend analysis. Mice were immunized with Bj2015. Antibodies were detected by double-immunodiffusion and total protein and albumin were measured. Neuromuscular blockade-induced by 40 mu g mL(-1) venom solution was carried out using mouse nerve phrenic-diaphragm preparation. Resulting muscles were submitted to light microscopy to evaluate the myotoxicity. PLA(2) activity of 0.1 mg mL(-1) Bj2015 was measured using 4-nitro-3-(octanoyloxy)benzoic acid as substrate. Over time, greater losses occurred in neurotoxicity than PLA(2), but not in myotoxicity and immunogenicity. Concluding, the neurotoxicity decrease can be related to enzymatic losses, including PLA(2). Depending on the purpose of use, the collected venom responds on a long time, avoiding unnecessary new collections, improving life quality of animals in captivity and increasing their longevity331624172421CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informação04/09705-8; 07/53883-6; 08/52643-4; 12/08271-0; 15/01420-
Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A2) Myotoxin
Purpose: Bothrops
snakes are responsible for more than 70 % of snakebites every year in Brazil
and their venoms cause severe local and systemic damages. The pharmacological
properties of medicinal plants have been widely investigated in order to
discover new alternative treatments for different classes of diseases including
neglected tropical diseases as envenomation by snakebites. In this work, we
have investigated the ability of Vochysia
haenkeana stem barks extract (VhE) to
neutralize the neuromuscular effects caused by Bothropstoxin-I (BthTX-I), the
major phospholipase A2 (PLA2) myotoxin from B. jararacussu venom.
Methods: The biological compounds of VhE were analysed
under thin layer chromatography (TLC) and its neutralizing ability against
BthTX-I was assessed through twitch-tension recordings and histological
analysis in mouse phrenic nerve-diaphragm (PND) preparations. The antimicrobial
activity of VhE was assessed against S.
aureus, E. coli and P. aeruginosa
strains. The aggregation activity of VhE was analysed under protein
precipitation assay.
Results: VhE showed the presence of phenolic compound
visualized by blue trace under TLC. VhE abolished the neuromuscular blockade
caused by BthTX-I applying the pre-toxin incubation treatment and partially
neutralized the BthTX-I action under post-toxin incubation treatment; VhE
contributed slightly to decrease the myotoxicity induced by BthTX-I. The
neutralizing mechanism of VhE may be related to protein aggregation. VhE showed
no antimicrobial activity.
Conclusion: V. haenkeana extract
which has no antimicrobial activity exhibited neutralizing ability against the
neuromuscular blockade caused by BthTX-I and also contributed to decrease its
myotoxicity. Protein aggregation involving phenolic compounds may be related in
these protective effects