Monitoramento da qualidade física de um argissolo sob plantio direto de milho e de mamona.

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Formulações dietéticas para cordeiros Morada Nova em confinamento, usando o NRC (2007): pesos e rendimentos de carcaça.

Resumo: Objetivou-se com este trabalho avaliar o uso e aplicação de formulações dietéticas para cordeiros Morada Nova, sob terminação em confinamento tomando por base o NRC (2007), sobre os pesos e rendimentos de carcaça. O experimento foi realizado na cidade de Sobral-CE entre dezembro/2013 e fevereiro/2014. Foram utilizados vinte animais, distribuídos em delineamento inteiramente ao acaso, em esquema fatorial 2 x 2 (duas dietas prescritas pelo NRC (2007) - maturidade precoce e tardia, com dois níveis de restrição de nutrientes ? 0% e 15%: Proteína Bruta-PB e Nutrientes Digestíveis Totais-NDT). O peso vivo ao abate foi obtido após jejum alimentar e hídrico por 18 horas. Após evisceração retirou-se a cabeça e a parte distal dos membros para obtenção do peso e rendimento de carcaça quente e rendimento biológico. Após resfriamento a 4°C por 24 horas, obteve-se o peso e rendimento da carcaça fria. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste Tukey a 5%. As dietas formuladas conforme o NRC (2007), apresentaram diferenças significativas (P0,05) os pesos e rendimentos de carcaça. A formulação baseada no NRC (2007) para maturidade precoce, reduzindo-se em 15% os teores de NDT e PB, garante o melhor peso vivo ao abate e os pesos e rendimentos das carcaças de cordeiros Morada Nova, sob terminação em confinamento. [Use and application of dietary formulations for Morada Nova lambs under feedlot finishing, based on NRC (2007): Weights and carcass yield]. Abstract: The study aimed to evaluate the use and application of dietary formulations for Morada Nova lambs, in feedlot finished based on NRC (2007), on the weights and carcass yield. The experiment was conducted in Sobral-Ceará, Brazil in period from 16th December 2013 to 21st February 2014. Twenty animals were distributed in a completely randomized design in a factorial scheme 2 x 2 (two diets prescribed by the NRC (2007) - early and late maturity, with two levels of restriction of both Crude Protein-CP and Total Digestible Nutrients-TDN ? 0% and 15%). Live weight at slaughter was obtained after 18h of food and water fasting. After evisceration, head and distal part of members were removed to obtain weight and hot carcass yield and biological yield. After cooling to 4 °C for 24 hours, weight and yield of the cold carcass were obtained. Data were subjected to analysis of variance and means compared by 5% Tukey test. Diets formulated according to NRC (2007) showed significant differences (P 0.05) weights and carcass yield. The formulation based on NRC (2007) for early maturity with reduction of 15% TDN and CP assures the best live weight at slaughter and carcass weight and yield of Morada Nova lambs in feedlot finished

Postmenopausal Osteoporosis reference genes for qPCR expression assays

Osteoporosis (OP) is a multifactorial disease influenced by genetic factors in more than half of the cases. In spite of the efforts to clarify the relationship among genetic factors and susceptibility to develop OP, many genetic associations need to be further functionally validated. Besides, some limitations as the choice of stably expressed reference genes (RG) should be overcome to ensure the quality and reproducibility of gene expression assays. To our knowledge, a validation study for RG in OP is still missing. We compared the expression levels, using polymerase chain reaction quantitative real time (qPCR) of 10 RG (G6PD, B2M, GUSB, HSP90, EF1A, RPLP0, GAPDH, ACTB, 18 S and HPRT1) to assess their suitability in OP analysis by using GeNorm, Normfinder, BestKeeper and RefFinder programs. A minimal number of two RG was recommended by GeNorm to obtain a reliable normalization. RPLP0 and B2M were identified as the most stable genes in OP studies while ACTB, 18 S and HPRT1 were inadequate for normalization in our data set. Moreover, we showed the dramatic effects of suboptimal RG choice on the quantification of a target gene, highlighting the importance in the identification of the most appropriate reference gene to specific diseases. We suggest the use of RPLP0 and B2M as the most stable reference genes while we do not recommend the use of the least stable reference genes HPRT1, 18 S and ACTB in OP expression assays using PBMC as biological source. Additionally, we emphasize the importance of individualized and careful choice in software and reference genes selection

Efeito da giberelina nas características dos cachos da uva 'Brasil' no Vale do São Francisco.

O presente trabalho teve como objetivo avaliar o efeito da aplicação de diferentes doses de giberelina, no diâmetro das bagas, no peso dos cachos, bem como na composição química dos frutos da cultivar Brasil