15 research outputs found
2 nd Brazilian Consensus on Chagas Disease, 2015
Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research
Hydrogel and membrane scaffold formulations of Frutalin (breadfruit lectin) within a polysaccharide galactomannan matrix have potential for wound healing
Plant lectins are carbohydrate-binding proteins, which can interact with cell surfaces to initiate anti-inflammatory pathways, as well as immunomodulatory functions. Here, we have extracted, purified and part-characterized the bioactivity of Jacalin, Frutalin, DAL and PNA, before evaluating their potential for wound healing in cultured human skin fibroblasts. Only Frutalin stimulated fibroblast migration in vitro, prompting further studies which established its low cytotoxicity and interaction with TLR4 receptors. Frutalin also increased p-ERK expression and stimulated IL-6 secretion. The in vivo potential of Frutalin for wound healing was then assessed in hybrid combination with the polysaccharide galactomannan, purified from Caesalpinia pulcherrima seeds, using both hydrogel and membrane scaffolds formulations. Physical-chemical characterization of the hybrid showed that lectin-galactomannan interactions increased the pseudoplastic behaviour of solutions, reducing viscosity and increasing Frutalin's concentration. Furthermore, infrared spectroscopy revealed -OH band displacement, likely caused by interaction of Frutalin with galactose residues present on galactomannan chains, while average membrane porosity was 100âŻÎŒm, sufficient to ensure water vapor permeability. Accelerated angiogenesis and increased fibroblast and keratinocyte proliferation were observed with the optimal hybrid recovering the lesioned area after 11âŻdays. Our findings indicate Frutalin as a biomolecule with potential for tissue repair, regeneration and chronic wound healing
Pólen 2n e mecanismos meióticos de formação em Solanum commersonii ssp 2n pollen and meiotic mechanisms of Formation in Solanum commersonii ssp
Solanum commersonii subsp. commersonii Dun. e Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 - 1EBN) nĂŁo podem ser diretamente cruzadas com a batata cultivada de S. tuberosum subsp. tuberosum L. (2n=4x=48 -4 EBN) em razĂŁo da barreira da ploidia e do nĂșmero de equilĂbrio do endosperma (EBN). No entanto, a produção de pĂłlen nĂŁo reduzido por S. commersonii ssp. (2x=2n=24 - 1EBN), permite o cruzamento desta com dihaplĂłides de batata e com as espĂ©cies diplĂłides S. phureja Juz. & Bukasov. e S. chacoense Bitt., que possuem 2 EBN. Conduziu-se este trabalho, com o objetivo de determinar o mecanismo meiĂłtico e a freqĂŒĂȘncia dos grĂŁos de pĂłlen nĂŁo reduzidos em cinco clones de Solanum commersonii ssp. Foram avaliados trĂȘs clones de S. commersonii subsp. commersonii (SCC) e dois de S. commersonii subsp. malmeanum (SCM). Nas avaliaçÔes, duzentos grĂŁos de pĂłlen viĂĄveis foram corados (carmim acĂ©tico 2%) e mensurados, sendo considerados nĂŁo reduzidos aqueles com o diĂąmetro igual ou superior a 25 ”m. O clone Solanum commersonii subsp. commersonii Dun. (SCC 100) produz 26.1% de polen nĂŁo reduzido, por fusos fundidos e fusos paralelos, o qual pode ser utilizado em programas de melhoramento.<br>Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 -1EBN) cannot be directly crossed with the cultivated Solanum tuberosum subsp. Tuberosum L. (2n=4x=48 - 4EBN) species due to ploidy barriers and endosperm balance number (EBN). However, non-reduced pollen production by the wild species S. commersonii ssp. (2x=2n=24 -1EBN), allows the crossing of these species with dihaploids of potato and with the diploids species, S. phureja Juz. & Bukasov. and S. chacoense Bitt., all with 2EBN. The aim of this work was to determine the meiotic mechanism and frequency of non-reduced pollen production of five clones of S. commersonii ssp. Three different clones of S. commersonii subsp. commersonii Dun. (SCC) and two of S. commersonii subsp. malmeanum Bitt. (SCM) were evaluated. In the evaluation, two hundred viable pollen grains were stained (2% acetic carmin) and measured. The pollen grains measuring 25 ”m in diameter or more were considered nonreduced. The clone Solanum commersonii subsp. commersonii Dun. (SCC 100) produces 26.1% of non-reduced pollen grains caused by parallel spindles and fusion spindles, which may be used in breeding program