Can P-glycoprotein and β-tubulin polymorphisms be used as genetic markers of resistance in Dirofilaria immitis from Rio de Janeiro, Brazil?

Submitted by Sandra Infurna ([email protected]) on 2018-08-30T17:01:44Z No. of bitstreams: 1 norma_LABARTHE_ETAL_ioc_2018.PDF: 1026902 bytes, checksum: 557a4e9a1ac3873af86f4f7889b1112d (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-08-30T17:12:22Z (GMT) No. of bitstreams: 1 norma_LABARTHE_ETAL_ioc_2018.PDF: 1026902 bytes, checksum: 557a4e9a1ac3873af86f4f7889b1112d (MD5)Made available in DSpace on 2018-08-30T17:12:22Z (GMT). No. of bitstreams: 1 norma_LABARTHE_ETAL_ioc_2018.PDF: 1026902 bytes, checksum: 557a4e9a1ac3873af86f4f7889b1112d (MD5) Previous issue date: 2018Universidade Federal Fluminense. Faculdade de Veterinária. Programa de Pós‑Graduação em Medicina Veterinária – Clínica e Reprodução Animal. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Programa de Pós‑Graduação em Medicina Veterinária – Clínica e Reprodução Animal. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Medicina e Cirurgia Veterinária. Seropédica, RJ, Brasil.Médica Veterinária, Vet Ypiranga. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Programa de Pós‑Graduação em Medicina Veterinária – Clínica e Reprodução Animal. Niterói, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Programa de Pós‑Graduação em Medicina Veterinária – Clínica e Reprodução Animal. Niterói, RJ, BrasilDirofilaria immitis, the causative agent of , is worldwide the most important filarid to affect domestic dogs. Prevention of this infection is done by macrocyclic lactones, but some reports on the lack of efficacy have been published. Although the actual cause of resistance is unknown, single nucleotide polymorphisms (SNPs) on a P-glycoprotein ABC transporter and β-tubulin genes have been pointed out as candidates for genetic markers of resistance. We conducted a survey to verify the presence of these suggested genetic markers in microfilariae from 30 naturally infected dogs under macrocyclic lactones treatment living in an endemic area in the state of Rio de Janeiro

Awareness of visceral leishmaniasis and its relationship to canine infection in riverside endemic areas in Northeastern Brazil

IntroductionAn awareness of visceral leishmaniasis (VL) is necessary to encourage the population to participate in prevention and control in collaboration with more efficient, centrally organized health programs. The aim of this study was to evaluate the awareness of the riverside population regarding VL and the association between awareness and the prevalence of canine visceral leishmaniasis (CVL).MethodsIn total, 71 people living in riverside areas in the City of Mossoró in State of Rio Grande do Norte participated of the study, and 71 dogs were tested for CVL by polymerase chain reaction (PCR). Association analysis of several variables related to knowledge of the riverside population regarding CVL positivity was performed, yielding odds ratios (OR) and 95% confidence intervals (CI), and significance was determined using chi-square (χ2) and Fisher's exact tests.ResultsAmong individuals whose dogs tested positive for CVL, 60% did not know the cure for CVL, and these subjects were three times more likely to have a dog test positive for CVL than those who were aware the cure for CVL. Knowledge of CVL cure was the only variable that remained in the logistic model after the successive removal of variables, with an adjusted OR of 3.11 (95%CI: 1.1-8,799; p=0.032).ConclusionsInsufficient awareness regarding VL in riverside areas with CVL-positive dogs was associated with increased rates of canine infection, which suggests that changes in habits and the adoption of attitudes and preventive practices may contribute to the control and prevention of this disease. This study reinforces the need to invest in better health education programs regarding VL

Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-09-18T15:13:39Z No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-09-18T15:24:52Z (GMT) No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5)Made available in DSpace on 2018-09-18T15:24:52Z (GMT). No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5) Previous issue date: 2018Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil