Effects of bodybuilding and protein supplements in saliva, gingival crevicular fluid, and serum

WOS: 000399263300015PubMed ID: 28367892The effects of bodybuilding and protein supplements on periodontal tissues have not yet been evaluated. The present study aimed to examine the periodontal status and interleukin (IL)-1 beta, apoptosis-associated speck-like protein containing C-terminal caspase-recruitment domain (ASC), and caspase 1 (CASP1) gene expression levels of body builders compared with those of controls. Twentyfive bodybuilders with gingivitis (BB-G) who used protein powder supplements were compared with 25 nonexercising males with (G) and 25 without (H) gingivitis. Saliva, gingival crevicular fluid (GCF), and serum were collected for gene expression analysis. Plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP) were recorded. GI and BOP were higher in group BB-G and G than in group H (P 0.05). In GCF, CASP1, ASC, and IL-1 beta expression were upregulated in group G compared with groups BB-G and H (P < 0.01). In addition, ASC (P < 0.05) and IL-1 beta (P < 0.01) were downregulated in group BB-G compared with group H. CASP1, IL-1 beta (P < 0.01), and ASC in the saliva were downregulated in group BB-G compared with groups H and G (P < 0.05). CASP1, IL-1 beta, and ASC may play a role in the pathogenesis of gingivitis. Bodybuilding and supplement usage may decrease gingival inflammation by downregulating CASP1, and ASC.Scientific Research Fund of Sifa University [SUBAP 2015-6]This study was supported by the Scientific Research Fund of Sifa University, Project Grant # SUBAP 2015-6

Quantification of circumferential bone level and extraction socket dimensions using different imaging and estimation methods: a comparative study

No studies have yet evaluated linear alveolar bone levels and extraction socket dimensions on dry skulls using different techniques. We aimed to investigate the accuracy of cone-beam computed tomography (CBCT), digital radiography, and digital photography

Effects of colchicine on gingival inflammation, apoptosis, and alveolar bone loss in experimental periodontitis

WOS: 000434135300010PubMed ID: 29520818Background: The aim of the study was to investigate the effects of colchicine on cytokine production, apoptosis, alveolar bone loss, and oxidative stress in an experimental model of periodontitis in rats. Methods: Forty-eight rats were divided equally into four groups: healthy (H); periodontitis (P); periodontitis+colchicine low dose (CL, 30 mu g/kg/day), and periodontitis+colchicine high dose (CH, 100 mu g/kg/day). After 11 days, interleukin (IL) -1 beta, IL-8, and IL-10 were analyzed in gingival samples using Enzyme-Linked ImmunoSorbent Assay. Receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), total oxidative stress (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured in gingiva and serum. Alveolar bone volume was evaluated via micro-CT. Apoptotic cells were detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in histological sections. Results: Colchicine treatment significantly reduced IL-1 beta, IL-8, RANKL, RANKUOPG, TOS, OSI, and bone volume ratio levels, and increased TAS levels compared to group P (p < 0.05). High dose colchicine treatment (CH) significantly decreased TUNEL+ cell counts compared to group P (p < 0.05). Conclusions: These finding suggest that colchicine has a prophylactic potential for the prevention of periodontal tissue destruction through anti-inflammatory, antioxidative, anti-apoptotic, and bone-protective effects.Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114S766]The authors declare that they have no conflicts of interest and they appreciate the financial support provided by The Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkey, Project Grant # 114S766