Quality control indicators of soil ridges at sowing cultivated crops

© 2016,International Journal of Pharmacy and Technology. All rights reserved.The initial component of any cultivated crops technology is high-quality preparation of a field in order to create proper conditions for the subsequent seed placement,plant root system’s growth and development stimulation. At the same time,any mechanical treatment should not disrupt the optimal soil structure; it should preserve the soil fertility,protect against erosion processes and maintain moisture to the greatest possible extent. Traditional technologies of growing cultivated crops on a flat field surface have been and remain the most widespread. However,the studies have found that the most promising is the ridge technology. A ridger seeder is developed for implementation of the ridge technology; the ridger seeder’s application allows to cost effectively perform presowing cultivation in just one pass,after sowing the seeds to form a soil mound over a stitch of sown seeds,to compact such soil mound on three sides and finally form a soil ridge of a required dimension and density. The tool’s flat disk and concave disks of the ridger seeder’s ridge forming roller have substantiated approach angles. It has been revealed that structural and operating parameters of the tools with flat discs and the ridge forming roller,as well as physical and mechanical properties of the soil affect the process of forming the soil ridges of required dimension and density at seeding any cultivated crops

Two Novel Amyloid Proteins, RopA and RopB, from the Root Nodule Bacterium Rhizobium leguminosarum

Amyloids represent protein fibrils with a highly ordered spatial structure, which not only cause dozens of incurable human and animal diseases but also play vital biological roles in Archaea, Bacteria, and Eukarya. Despite the fact that association of bacterial amyloids with microbial pathogenesis and infectious diseases is well known, there is a lack of information concerning the amyloids of symbiotic bacteria. In this study, using the previously developed proteomic method for screening and identification of amyloids (PSIA), we identified amyloidogenic proteins in the proteome of the root nodule bacterium Rhizobium leguminosarum. Among 54 proteins identified, we selected two proteins, RopA and RopB, which are predicted to have β-barrel structure and are likely to be involved in the control of plant-microbial symbiosis. We demonstrated that the full-length RopA and RopB form bona fide amyloid fibrils in vitro. In particular, these fibrils are β-sheet-rich, bind Thioflavin T (ThT), exhibit green birefringence upon staining with Congo Red (CR), and resist treatment with ionic detergents and proteases. The heterologously expressed RopA and RopB intracellularly aggregate in yeast and assemble into amyloid fibrils at the surface of Escherichia coli. The capsules of the R. leguminosarum cells bind CR, exhibit green birefringence, and contain fibrils of RopA and RopB in vivo.This work was supported by the Russian Science Foundation, grant 17-16-01100

Quality control indicators of soil ridges at sowing cultivated crops

© 2016,International Journal of Pharmacy and Technology. All rights reserved.The initial component of any cultivated crops technology is high-quality preparation of a field in order to create proper conditions for the subsequent seed placement,plant root system’s growth and development stimulation. At the same time,any mechanical treatment should not disrupt the optimal soil structure; it should preserve the soil fertility,protect against erosion processes and maintain moisture to the greatest possible extent. Traditional technologies of growing cultivated crops on a flat field surface have been and remain the most widespread. However,the studies have found that the most promising is the ridge technology. A ridger seeder is developed for implementation of the ridge technology; the ridger seeder’s application allows to cost effectively perform presowing cultivation in just one pass,after sowing the seeds to form a soil mound over a stitch of sown seeds,to compact such soil mound on three sides and finally form a soil ridge of a required dimension and density. The tool’s flat disk and concave disks of the ridger seeder’s ridge forming roller have substantiated approach angles. It has been revealed that structural and operating parameters of the tools with flat discs and the ridge forming roller,as well as physical and mechanical properties of the soil affect the process of forming the soil ridges of required dimension and density at seeding any cultivated crops

Quality control indicators of soil ridges at sowing cultivated crops

© 2016,International Journal of Pharmacy and Technology. All rights reserved.The initial component of any cultivated crops technology is high-quality preparation of a field in order to create proper conditions for the subsequent seed placement,plant root system’s growth and development stimulation. At the same time,any mechanical treatment should not disrupt the optimal soil structure; it should preserve the soil fertility,protect against erosion processes and maintain moisture to the greatest possible extent. Traditional technologies of growing cultivated crops on a flat field surface have been and remain the most widespread. However,the studies have found that the most promising is the ridge technology. A ridger seeder is developed for implementation of the ridge technology; the ridger seeder’s application allows to cost effectively perform presowing cultivation in just one pass,after sowing the seeds to form a soil mound over a stitch of sown seeds,to compact such soil mound on three sides and finally form a soil ridge of a required dimension and density. The tool’s flat disk and concave disks of the ridger seeder’s ridge forming roller have substantiated approach angles. It has been revealed that structural and operating parameters of the tools with flat discs and the ridge forming roller,as well as physical and mechanical properties of the soil affect the process of forming the soil ridges of required dimension and density at seeding any cultivated crops

Experience of image-analysis of rye grain

The article presents the results of image analysis of size and color of rye grains of different color that is controlled by known genes. It was studied 23 samples of rye with the color of the grain visually described as yellow, green, brown and violet. Software GrainScan developed for wheat was adapted for computer analysis of digital images of rye kernels obtained with flatbed scanner. Software along with size allows calculating three-coordinates of color in three-dimensional color space CIEL*a*b*. Based on coordinate values a* and b* calculation of two additional parameters - C* and h* describing the saturation (purity) and tone (the color itself) of the grains, respectively, was made. It is shown that the individual analysis of size and color characteristics of the kernels give low values of standard errors. This allows revealing pairs of forms, which differ from each other for any of the studied parameters. The color of the rye grain cannot be described as the saturated color of one of the tones. In accordance to values of h*, it was possible to divide four visually selected groups into three ones. A composition of groups correspond to lack of anthocyanins in caryopsis (anthocyaninless, yellow-seeded, brown-seeded samples), the presence of anthocyanins in the aleurone layer (green-seeded samples) and the presence of anthocyanins in the pericarp (violet-seeded samples). The data obtained are discussed in connection with literature data on visual description of colors of rye grain, type of pigments, and the coloring of the individual layers of rye kernel

BONE GRAFTING ENHANCED BY PLATELET-RICH PLASMA IN TREATMENT OF AVASCULAR NECROSIS OF FEMORAL HEAD

Treatment of avascular necrosis of the femoral head is an issue of current interest while it affects young and employable people. So far there is no well-defined strategy of management which would help to postpone hip arthroplasty and further revision procedure. Hip sparing surgical treatment of avascular necrosis of the femoral head by bone grafting prior to head collapse proved to be a viable option not only during early stages of disease but also at advanced stages. Platelet-rich plasma (PRP) addition to treatment plan potentially helps improving bone regeneration in situ.In this article the authors present a case of a 37 years old patient with avascular necrosis of the femoral head at a fragmentation stage (type 4B by ARCO). The authors centrifuged 15 ml of autologous whole blood (1500 RPM) obtained by a special double-contoured syringe. During the surgical stage of treatment PRP and morselized bone graft were mixed to introduce and impact into the debrided zone of avascular necrosis. The authors also introduced 0.3–0.4 ml of PRP into the debrided zone of avascular necrosis after bone grafting. At 6 months follow-up CT images of the studied patient demonstrated signs of bone reorganization and no loss of femoral head sphericity. Preoperative Visual Analogue Scale (VAS), Harris Hip Score (HHS) and Hip disability and Osteoarthritis Outcome Score (HOOS) prior to treatment were 60, 45 and 33 points respectively. Postoperative VAS, HHS and HOOS scores were 10, 78 and 78 respectively. In the authors’ opinion, impaction bone grafting enhanced by PRP helps obtaining good and excellent outcomes not only at early but also at advanced stages of avascular necrosis

Dry fermented vegetable products of long-term storage and their integration into the diet of astronauts

Relevance. Fermentation makes it possible to improve the properties of the final product, increase its nutritional value and organoleptic characteristics. It has been scientifically proven that the use of fermented foods (with high nutritional and biological value) on a regular basis has a positive effect on human health, increases immunity, improves the functioning of the gastrointestinal tract. Dry fermented products with a long shelf life can be recommended as part of the diet in space.   The aim is to creation of a dry base for fermented long-term storage beverages based on cabbage and table carrots, applicable in the diet of space nutrition.   Objects and methods of research. The objects of the study were the domestic hybrid of white cabbage F1 Severyanka and the domestic variety of carrot table Margosha, and the dry fermented products obtained from them.   Results. It was found that, according to biochemical and organoleptic quality indicators, the domestic hybrid of white cabbage F1 Severyanka and the domestic carrot variety Margosha are suitable for various types of processing, including fermentation and manufacture of dried fermented products. The comparison of the number of microorganisms in fermented cabbage and cabbage dehydrated by LTVD (low temperature vacuum drying) and VFD (vacuum freeze drying methods was carried out. The survival rate of microorganisms was 1.62 times higher in LTVD. The ways of accelerating the fermentation process of table carrots are considered. The expediency of using starter cultures obtained on the basis of the fermented product has been confirmed. It has been found that using starter cultures in the form of a fermented product dried by the LTVD method accelerates fermentation and allows you to reach the desired pH level 1.33 times faster than the base one and does not affect the final organoleptic characteristics

Anthocyanin Composition and Content in Rye Plants with Different Grain Color

The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1...vi6) lead to the absence of anthocyanins in all parts of the plant. Moreover, dominant genes of anthocyanin synthesis in aleurone (gene C) and pericarp (gene Vs) also affect the color of the grain. Reverse phase high-performance liquid chromatography and mass spectrometry were used to study anthocyanins in 24 rye samples. A lack of anthocyanins in the lines with yellow and brown grain was determined. Delphinidin rutinoside and cyanidin rutinoside were found in the green-seeded lines. Six samples with violet grains significantly varied in terms of anthocyanin composition and content. However, the main aglycone was cyanidin or peonidin in all of them. Monosaccharide glucose and disaccharide rutinose served as the glycoside units. Violet-seeded accession forms differ in the ratio of the main anthocyanins and the range of their acylated derivatives. The acyl groups were presented mainly by radicals of malonic and sinapic acids. For the colored forms, a profile of the revealed anthocyanins with the indication of their contents was given. The obtained results are discussed in connection to similar data in rice, barley, and wheat, which will provide a perspective for future investigations